- Ready-to-use Comet Slides allow direct application of sample without pretreatment
- Shorter assay time allows for higher throughput sample analysis
- Hydrophobic barrier allows sample treatment with DNA repair enzymes
- Unique nucleic acid stain provides improved sensitivity for DNA visualization compared to ethidium bromide
Exposure of cells to oxidative and environmental stresses frequently results in the breakdown or oxidation of genomic DNA. Assays to evaluate the integrity of genomic DNA, or to assess the presence of oxidized DNA are frequently used as a means of verifying the onset of apoptosis or DNA damage. The Comet SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic "comet" shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA. The Comet SCGE Assay is a fast and simple electrophoresis method to detect and quantitate DNA fragmentation in cells associated with DNA damage and apoptosis. A unique nucleic acid stain, CYGREEN® Nucleic acid dye, provides improved sensitivity for DNA visualization compared to ethidium bromide.
Negative control. CaSki cells were untreated. Comet SCGE Assay was performed and slides were imaged using a FITC filter.
Positive control. CaSki cells were treated with 100mM hydrogen peroxide for 20 min at 4°C. Comet SCGE Assay was performed and slides were imaged using a FITC filter.
Positive control. CaSki cells were treated with 100mM hydrogen peroxide for 20 min at 4°C. Comet SCGE Assay was performed and slides were imaged using a FITC filter.
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Product Details
| Assay Time: | 3 hours |
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| Applications: | Fluorescence microscopy
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| Application Notes: | For the measurement of DNA Damage, DNA Repair, and Apoptosis in cells and tissue from any species via electrophoresis. |
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| Species reactivity: | Species independent
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| Use/Stability: | Store CYGREEN® Nucleic Acid Dye at -20°C. All other reagents are stable at room temperature. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | Ambient |
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| Contents: | Lysis solution, Comet LMAgarose, Comet slide, EDTA, CYGREEN® Nucleic acid dye |
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| Technical Info/Product Notes: | Please note that CYGREEN® Nucleic acid dye is now included with the Comet SCGE assay kit. Please follow the recommended staining procedures in the updated manual. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
SN-38 Sensitizes BRCA-Proficient Ovarian Cancers to PARP Inhibitors through Inhibiting Homologous Recombination Repair: S. Lin, et al.; Dis. Markers
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Therapeutic Targeting of MYC in Head and Neck Squamous Cell Carcinoma: S. Liu, et al.; Oncoimmunology
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Assessment of mutations induced by cold atmospheric plasma jet treatment relative to known mutagens in Escherichia coli: B.L. Patenall, et al.; Mutagenesis
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Bleomycin induces fibrotic transformation of bone marrow stromal cells to treat height loss of intervertebral disc through the TGFβR1/Smad2/3 pathway: X. Yang, et al.; Stem Cell Res. Ther.
12, 34 (2021),
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Chronic exposure to Cytolethal Distending Toxin (CDT) promotes a cGAS-dependent type I interferon response: B.J. Pons, et al.; Cell. Mol. Life Sci.
78, 6319 (2021),
Abstract;
Rising levels of antioxidative phyllobilins in stored agricultural produce and their impact on consumer acceptance: C.A. Karg, et al.; NPJ Sci. Food
5, 1038 (2021),
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Sanguinarine mediated apoptosis in Non-Small Cell Lung Cancer via generation of reactive oxygen species and suppression of JAK/STAT pathway: K.S. Prabhu, et al.; Biomed. Pharmacother.
144, 112358 (2021),
Abstract;
Robust elimination of genome-damaged cells safeguards against brain somatic aneuploidy following Knl1 deletion: L. Shi, et al.; Nat. Commun.
10, 2588 (2019),
Abstract;
Full Text
Marine fungal DHICA as a UVB protectant: Assessment under in vitro and in vivo conditions: S.K. Shanuja, et al.; J. Photochem. Photobiol. B.
179, 139 (2018),
Application(s): Use with irradiated HaCaT cells,
Abstract;
NF-κB and Poly (ADP-ribose) Polymerase 1 Form a Positive Feedback Loop that Regulates DNA Repair in Acute Myeloid Leukemia Cells: D. Li, et al.; Mol. Cancer Res.
17, 761 (2018),
Abstract;
Selective toxicity of tumor treating fields to melanoma: an in vitro and in vivo study: Y. Jo, et al.; Cell Death Dis.
5, 46 (2018),
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Full Text
STAG2 deficiency induces interferon responses via cGAS-STING pathway and restricts virus infection: S. Ding, et al.; Nat. Commun.
9, 1485 (2018),
Abstract;
Full Text
Cleavage of Ku80 by caspase-2 promotes non-homologous end joining-mediated DNA repair: Q. Yan, et al.; DNA Repair (Amst.)
60, 18 (2017),
Abstract;
Development of novel alternative biodiesel fuels for reducing PM emissions and PM-related genotoxicity: P.M. Yang, et al.; Environ. Res.
156, 512 (2017),
Abstract;
p21 maintains senescent cell viability under persistent DNA damage response by restraining JNK and caspase signaling: R. Yosef, et al.; EMBO J.
36, 2280 (2017),
Application(s): Use with IMR-90 and BJ fibroblasts,
Abstract;
Full Text
DNA-Bioprotective Effects of Lactic Acid Bacteria Against Aflatoxin B: S. Kurban, et al.; Curr. Res. Nutr. Food Sci. 4, 87 (2016),
Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells: J.H. Kim, et al.; Int. J. Nanomedicine
10, 5513 (2015),
Application(s): Assay trypsinizing and analyzing human cells,
Abstract;
Full Text
Cytotoxic, genotoxic, and neurotoxic effects of Mg, Pb, and Fe on pheochromocytoma (PC‐12) cells: T. Sanders, et al.; Environ. Toxicol.
30, 1445 (2015),
Abstract;
Full Text
Selective inhibition of esophageal cancer cells by combination of HDAC inhibitors and azacytidine: T.D. Ahrens, et al.; Epigenetics
10, 431 (2015),
Application(s): Use with esophageal cell lines,
Abstract;
Full Text
Listeria monocytogenes dampens the DNA damage response: A. Samba-Louaka, et al.; PLoS Pathog.
10, e1004470 (2014),
Application(s): Use with HeLa cells,
Abstract;
Full Text
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