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Comet SCGE assay kit

Fast and simple electrophoresis method to measure DNA fragmentation
 
ADI-900-166 50 tests 270.00 USD
Bulk Request
 
  • Ready-to-use Comet Slides allow direct application of sample without pretreatment
  • Shorter assay time allows for higher throughput sample analysis
  • Hydrophobic barrier allows sample treatment with DNA repair enzymes
  • Unique nucleic acid stain provides improved sensitivity for DNA visualization compared to ethidium bromide
Exposure of cells to oxidative and environmental stresses frequently results in the breakdown or oxidation of genomic DNA. Assays to evaluate the integrity of genomic DNA, or to assess the presence of oxidized DNA are frequently used as a means of verifying the onset of apoptosis or DNA damage. The COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic "comet" shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA. The COMET SCGE Assay is a fast and simple electrophoresis method to detect and quantitate DNA fragmentation in cells associated with DNA damage and apoptosis. A unique nucleic acid stain, CYGREEN™ Nucleic acid dye, provides improved sensitivity for DNA visualization compared to ethidium bromide.
ADI-900-166 neg1
Negative control. CaSki cells were untreated. Comet SCGE Assay was performed and slides were imaged using a FITC filter.
ADI-900-166 pos1
Positive control. CaSki cells were treated with 100mM hydrogen peroxide for 20 min at 4°C. Comet SCGE Assay was performed and slides were imaged using a FITC filter.
ADI-900-166 pos2
Positive control. CaSki cells were treated with 100mM hydrogen peroxide for 20 min at 4°C. Comet SCGE Assay was performed and slides were imaged using a FITC filter.
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ADI-900-166 neg1 ADI-900-166 pos1 ADI-900-166 pos2

Product Specification

Assay Time:3 hours
 
Applications:Fluorescence microscopy
 
Application Notes:For the measurement of DNA Damage, DNA Repair, and Apoptosis in cells and tissue from any species via electrophoresis.
 
Species reactivity:Species independent
 
Use/Stability:Store all components ambient temperature.
 
Long Term Storage:Ambient
 
Kit/Set Contains:Lysis solution, Comet LMAgarose, Comet slide, EDTA, CYGREEN™ Nucleic acid dye
 
Technical Info/Product Notes:Please note that CYGREEN™ Nucleic acid dye is now included with the COMET SCGE assay kit. Please follow the recommended staining procedures in the updated manual.
 

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CYGREEN™ Nucleic acid dye 

Nucleic acid dye for gel staining and higher sensitivity qPCR
≥93% (HPLC), qPCR, Gel staining, Melting curve | Print as PDF
 
ENZ-GEN105-0100 100 µl 100.00 USD
Bulk Request
 

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