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cGMP complete ELISA kit

Most sensitive (0.043 pmol/ml) and complete colorimetric kit for intra- and extracellular cGMP quantification.
 
ADI-900-164 96 wells 416.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-901-164 (5x96 wells)
  • Versatile and convenient kit to assay both intracellular and extracellular cGMP
  • High sensitivity with an optional acetylation protocol which increases sensitivity 10-fold (0.059 pmol/ml)
  • Reliable and consistent lot-to-lot performance
  • Higher throughput format with results in just 3 hours for up to 39 samples in duplicate
  • Simple, efficient and well-established sample handling protocols
This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cGMP diluted in buffer or intracellular cGMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm. The ready-to-use liquid color-coded reagents provided reduce error.

Product Specification

Alternative Name:cyclic GMP
 
Sensitivity:Assay Buffer : 0.42 pmol/ml (non-acetylated) 0.043 pmol/ml (acetylated) HCl: 0.604 pmol/ml (non-acetylated) 0.059 pmol/ml (acetylated) (range 0.8 - 500 pmol/ml (non-acetylated); 0.08 - 50 pmol/ml (acetylated))
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of cGMP in cell lysates, culture supernatants, plasma, serum, saliva, tissue, and urine from any species.
 
Species reactivity:Species independent
 
Crossreactivity:cyclic GMP (100%) and <0.001%: GMP, GTP, cyclic AMP, AMP, ATP, cyclic UMP, CTP
 
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:GxR IgG Microtiter plate, Conjugate, Antibody, 0.1M HCl, Assay buffer 2, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Triethylamine, Acetic anhydride
 
Scientific Background:Cyclic GMP (cGMP), which has been shown to be present at levels typically 10-100 fold lower than cAMP, is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones, such as Acetylcholine, Insulin, and Oxytocin, as well as certain other chemicals like Serotonin and Histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate, and nitric oxide (NO) also stimulate cGMP levels.
 

Product Literature References

cGMP/PKG Signaling Suppresses Inositol 1,4,5-Trisphosphate Receptor Phosphorylation and Promotes Endoplasmic Reticulum Stress in Photoreceptors of CNG Channel-Deficient Mice: H. Ma, et al.; J. Biol. Chem. 290, 20880 (2015), Application(s): ELISA using mouse retinal lysate, Abstract; Full Text
Heterozygous NPR2 mutations cause disproportionate short stature, similar to Léri-Weill dyschondrosteosis: A. Hisado-Oliva, et al.; J. Clin. Endocrinol. Metab. 100, E1133 (2015), Application(s): ELISA using primate supernatant, Abstract; Full Text
Subcellular Trafficking of Guanylyl Cyclase/Natriuretic Peptide Receptor-A with Concurrent Generation of Intracellular cGMP: I. Mani, et al.; Biosci. Rep. 35, e00260 (2015), Application(s): cGMP ELISA using human cells, Abstract; Full Text
cGMP inhibition of type 3 phosphodiesterase is the major mechanism by which C-type natriuretic peptide activates CFTR in the shark rectal gland: H.R. De Jonge, et al.; Am. J. Physiol. Cell Physiol. 306, C343 (2014), Application(s): ELISA using dogfish shark supernatant, Abstract; Full Text
cGMP accumulation causes photoreceptor degeneration in CNG channel deficiency: evidence of cGMP cytotoxicity independently of enhanced CNG channel function: J. Xu, et al.; J. Neurosci. 33, 14939 (2013), Application(s): ELISA using mouse retinal lysate, Abstract; Full Text

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