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p21 (human), ELISA kit

 
ADI-900-161 96 wells 562.00 USD
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  • Easy-to-use - liquid color-coded reagents reduce error
  • Sensitive - quantify picogram levels compared to microgram levels in Western blot
  • Reproducible - excellent lot-to-lot consistency
The p21 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.

Product Specification

Alternative Name:Cip1
 
Sensitivity:8.0 pg/ml (range 15.6 - 1,000 pg/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of human p21 in cell lysates.
 
Species reactivity:Human
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 23 concentrate, Wash buffer concentrate, Standard, TMB Substrate, RIPA Cell lysis buffer 2, Stop solution 2
 
Scientific Background:Progression through the cell cycle is controlled by cyclin-dependent kinases (CDKs). Human p21WAF1/CIP1 is a CDK inhibitor that was first cloned and characterized as a mediator of p53-induced growth arrest, and serves as a regulator of cell cycle progression, terminal differentiation, and apoptosis. Human p21 inhibits cyclin-CDK2 and cyclin-CDK4 complexes, regulating cell cycle progression in G1 phase. p21 expression can be induced independently of p53 by TGF-β, tamoxifen, progesterone, nerve growth factor and apicidin. Human p21 expression has been implicated in a variety of human cancers including those of the prostate, bladder, and esophagus.
 
UniProt ID:P38936
 

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