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Glutathione (GSSG/GSH), detection kit

Rapid, ultra-sensitive kit for the quantification of reduced (GSH), oxidized (GSSG), and total glutathione for oxidative stress research.
 
ADI-900-160 4x96 wells 402.00 USD
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  • Highly sensitive enzymatic recycling reaction measures total, oxidized, or reduced glutathione
  • Easy-to-use sample handling protocols for a wide variety of matrices
  • Convenient and colorimetric 96 well plate format
The Glutathione (GSSG/GSH), detection kit provides a highly sensitive enzymatic recycling reaction for the measurement of total, oxidized, or reduced glutathione in a convenient 96-well plate format.

Product Specification

Sensitivity:6.25 pmoles/well (range 12.5 - 100 pmoles/well)
 
Applications:Colorimetric detection
 
Application Notes:For the measurement of total, oxidized and reduced forms of glutathione in cell lysates, plasma, saliva, tissue, urine, and whole blood from any species.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:Microtiter plate, Assay buffer concentrate, Reaction mix, Glutathione reductase, GSSG
 
Scientific Background:Glutathione, a major intracellular non-protein thiol, protects against free radical damage by providing reducing equivalents for several key antioxidant enzymes. In addition, glutathione acts as a scavenger of hydroxyl radicals and nascent oxygen. Elevated glutathione levels are associated with a reduced rate of illness, higher levels of self-rated health, lower cholesterol, lower body mass index, and lower blood pressure among the elderly. Glutathione provides a primary defense system for the removal of oxidants in the brain. Studies have revealed a correlation between low glutathione levels and damage to neurons that manufacture dopamine, suggesting a link to Parkinson's disease.
 

Product Literature References

Ethanol Extract of Cirsium japonicum var. ussuriense Kitamura Exhibits the Activation of Nuclear Factor Erythroid 2-Related Factor 2-dependent Antioxidant Response Element and Protects Human Keratinocyte HaCaT Cells Against Oxidative DNA Damage: O.K. Yoo, et al.; J. Cancer Prev. 21, 66 (2016), Application(s): Determination of intracellular reduced glutathione level, Abstract; Full Text
Novel triterpenoid AECHL-1 induces apoptosis in breast cancer cells by perturbing the mitochondria-endoplasmic reticulum interactions and targeting diverse apoptotic pathways: M.A. Sawant, et al.; Biochim. Biophys. Acta 1860, 1056 (2016), Application(s): Detection of total GSH levels in vivo, Abstract;
Prominent role of DPP III in the E2-mediated protection against hyperoxia in liver of female CBA/H mice: S. Sobočanec , et al.; Redox Biol. 8, 149 (2016), Application(s): Determination of glutathione level in liver cells of mice, Abstract;
Protection of Chinese olive fruit extract and its fractions against advanced glycation endproduct-induced oxidative stress and pro-inflammatory factors in cultured vascular endothelial and human monocytic cells: C.T. Kuo, et al.; J. Funct. Foods 27, 526 (2016), Application(s): EA.hy926 cells ,
Ameliorating effects of ethyl acetate fraction from onion (Allium cepa L.) flesh and peel in mice following trimethyltin-induced learning and memory impairment: S. K. Park, et al.; Food Res. Int. 75, 53 (2015), Application(s): Total glutathione measured in mouse brain tissue homogenate,
Antiamnesic Effect of Actinidia arguta Extract Intake in a Mouse Model of TMT-Induced Learning and Memory Dysfunction: J.S. Ha, et al.; Evid. Based Complement. Alternat. Med. Article ID 876484 (2015), Application(s): Determination of GSH and oxidized glutathione dimer in mouse brain, Abstract;
Catechin-7-O-β-D-glucopyranoside isolated from the seed of Phaseolus calcaratus Roxburgh ameliorates experimental colitis in rats: S.H. Kook, et al.; Int. Immunopharmacol. 29, 521 (2015), Abstract;
Celastrol prevents circulatory failure via induction of heme oxygenase-1 and heat shock protein 70 in endotoxemic rats: Y.L. Wang, et al.; J. Ethnopharmacol. 162, 168 (2015), Application(s): Assay, Abstract;
Evaluation of gene expression changes in human primary lung epithelial cells following 24-hr exposures to inorganic arsenic and its methylated metabolites and to arsenic trioxide: A.Y. Efremenko, et al.; Environ. Mol. Mutagen. 56, 477 (2015), Abstract;
Mancozeb affects mitochondrial activity, redox status and ATP production in mouse granulosa cells: R. Iorio, et al.; Toxicol. In Vitro 30, 438 (2015), Abstract;
Effect of the combination of metformin hydrochloride and melatonin on oxidative stress before and during pregnancy, and biochemical and histopathological analysis of the livers of rats after treatment for polycystic ovary syndrome: A.J. Lemos, et al.; Toxicol. Appl. Pharmacol. 14, 212 (2014), Abstract;
The Chinese herbal formula Liuwei dihuang protects dopaminergic neurons against Parkinson's toxin through enhancing antioxidative defense and preventing apoptotic death: Y.T. Tseng, et al.; Phytomedicine 21, 724 (2014), Application(s): Total GSH measured in rat primary mesencephalic neuron lysates, Abstract;
Establishing the thermal threshold of the tropical mussel Perna viridis in the face of global warming: B.P. Goh & C.H. Lai; Mar. Pollut. Bull. 13, 662 (2013), Abstract;
Insulin-like growth factor I (IGF-I)-induced chronic gliosis and retinal stress lead to neurodegeneration in a mouse model of retinopathy: P. Villacampa, et al.; J. Biol. Chem. 288, 24 (2013), Abstract; Full Text
Continuous presence of H₂O₂ induces mitochondrial-mediated, MAPK- and caspase-independent growth inhibition and cytotoxicity in human gingival fibroblasts: J.Y. Yu, et al.; Toxicol. In Vitro 26, 561 (2012), Application(s): Used with human gingival fibroblasts (HGF) and human YD-38 cell lysates., Abstract;
Continuously generated H2O2 stimulates the proliferation and osteoblastic differentiation of human periodontal ligament fibroblasts: Y. Choe, et al.; J. Cell. Biochem. 113, 1426 (2012), Application(s): Used with human periodontal ligament fibrobast (PLF) lysates., Abstract; Full Text
Elevated circulating angiogenic progenitors and white blood cells are associated with hypoxia-inducible angiogenic growth factors in children with sickle cell disease: S.F. Ofori-Acquah, et al.; Anemia 2012, (2012), Abstract; Full Text

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