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TGF-β1 ELISA kit

ADI-900-155 96 wells 493.00 USD
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  • Sensitive - measure as little as 3.3 pg/ml
  • Reliable - fully validated in activated serum, plasma, and culture supernatants
  • Simple - liquid color-coded reagents and pre-coated plates reduce error
The TGF-β1 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4 hours. Absorbance is read at 450 nm. This kit provides ultra-sensitive measurement of as little as 3.3 pg/mL TGF-β1 and has been fully validated in activated serum, plasma, and culture supernatants. The ready-to-use liquid color-coded reagents reduce error.
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Product Specification

Alternative Name:Transforming growth factor β 1
Sensitivity:3.3 pg/ml (range 31.25 - 1000 pg/ml)
Assay Time:4 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of TGF-β1 in culture supernatants, plasma, and serum of human, mouse, rat, and cow origin.
Species reactivity:Human, Mouse, Rat
Crossreactivity:Human TGF-β1 (100%), TGF-β1.2 (2.23%), latent TGF-β1 (<0.71%) and <0.03%: TGF-β2, TGF-β3, LAP
Use/Stability:Store all components at +4°, except standard at -20°.
Shipping:Shipped on Blue Ice
Contents:Microtiter plate, Conjugate, Antibody, Assay buffer 13, Assay buffer 30, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2
Scientific Background:Transforming growth factor beta 1 is also known as TGFβ1, TGF β, and TGFB. The inactive form consists of a TGF-β1 homodimer that is non-covalently linked to a latency-associated peptide homodimer. The active form is a homodimer of mature TGF-β1 that is disulfide linked. The precursor is cleaved into mature TGF-β1 and the latency-associated peptide. Many cells can synthesize TGF-β1, which is secreted to function in proliferation, differentiation, transformation, signaling and apoptosis. This cytokine has been implicated in many diseases including diabetes, renal disease, chronic pulmonary obstructive disease, as well as many cancers including prostate, and colon. This protein is a potential tumor-specific cancer therapeutic. Specifically, as a stable soluble dimer, it selectively induces apoptosis in many transformed cells, but not in normal cells. The literature contains many reviews on the biology and function of TGF-β1.
UniProt ID:P01137

Product Literature References

Pirfenidone enhances the efficacy of combined radiation and sunitinib therapy: S.H. Choi, et al.; Biochem. Biophys. Res. Commun. 462, 138 (2015), Application(s): ELISA using mouse culture medium, Abstract;
The effect of IFN-γ and TGF-β in the functional activity of mononuclear cells in the presence of Entamoeba histolytica: L. Campelo Albuquerque Moraes, et al. ; Parasit. Vectors 8, 413 (2015), Application(s): ELISA using culture supernatant of human MN cells, Abstract; Full Text
Angiotensin II-induced pro-fibrotic effects require p38MAPK activity and transforming growth factor beta 1 expression in skeletal muscle cells: M.G. Morales, et al.; Int. J. Biochem. Cell. Biol. 44, 1993 (2012), Application(s): ELISA using culture supernatants, Abstract;

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