- Specific - does not detect vasopressin, providing confidence in assay results
- Sensitive - detect < 12 pg/ml oxytocin
- Easy-to-use - liquid color-coded reagents and pre-coated plates reduce errors
- Trusted - highly published in peer reviewed literature
The Oxytocin EIA kit is a colorimetric competitive enzyme immunoassay kit with results overnight + 1 hour. Absorbance is read at 405 nm. Very low reactivity with vasopressin provides confidence in assay results. Sensitive quantification of < 12 pg/mL oxytocin. Ready-to-use liquid color-coded reagents reduce errors in the lab.
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Product Specification
| Sensitivity: | 11.7 pg/ml (range 15.6 - 1,000 pg/ml) |
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| Kit/Set Contains: | GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
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| Application: | For the quantitative determination of Oxytocin in culture supernatants, milk, plasma, and serum from any species. Cited sample types include cerebral spinal fluid (ref. 1), saliva (ref. 2, 4), and tissue (ref. 1). |
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| Use/Stability: | Store all components at +4°, except standard and conjugate at -20°. |
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| Miscellaneous/General: | Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. A hormone once thought to be limited to female smooth muscle reproductive physiology, more current findings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social/sexual behavior, and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg-Vasopressin, maintain renal water and sodium balance. |
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Product Literature References
Evaluation of Enzyme Immunoassay and Radioimmunoassay Methods
for the Measurement of Plasma Oxytocin: A. Szeto, et al.; Psychosomatic Medicine
73, 393 (2011),
Abstract;
The cross-generation transmission of oxytocin in humans: R. Feldman, I. Gordon, & O. Zagoory-Sharon; Hormones and Behavior
58, 669 (2010), Application(s): EIA using human saliva (ref. 4).,
Abstract;
Influence of a 'Warm Touch' Support Enhancement Intervention Among Married Couples on Ambulatory Blood Pressure, Oxytocin, Alpha Amylase,and Cortisol: J. Holt-Lunstad, et al. ; Psychosom. Med.
70, 976 (2008), Application(s): EIA using human plasma and saliva (ref. 2),
Abstract;
Monetary sacrifice among strangers is mediated by endogenous oxytocin release after physical contact: P. Zak, et al. ; Evol. Hum. Behav. 29, 375 (2008), Application(s): EIA using human plasma and serum.,
PVN electrical stimulation prolongs withdrawal latencies and releases oxytocin in cerebrospinal fluid, plasma, and spinal cord tissue inintact and neuropathic rats: M. Condes-Lara, et al. ; Pain
140, 265 (2008), Application(s): EIA using rat cerbrospinal fluid, plasma and tissue (ref. 1),
Abstract;