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Cytochrome c (human), ELISA kit

 
ADI-900-141 96 wells 572.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ALX-850-261

  • Reliable - fully validated in-house with both cytosolic and mitochondrial cell fractions
  • Ready-to-use - cell fractionation reagents provided
  • Higher throughput - save time and sample with fully quantitative results and increased sensitivity compared to Western blot
  • Fast - results from up to 40 samples in duplicate in ~3 hours
The Cytochrome c (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours 15 minutes. Absorbance is read at 405 nm. This kit has been validated in-house with both cytosolic and mitochondrial cell fractions and is supplied with the cell fractionation buffers. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
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Product Specification

Sensitivity:6.03 pg/ml (range 28.13 - 900 pg/ml)
 
Assay Time:3 hours 15 minutes
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of human Cytochrome c in cell lysates
 
Species reactivity:Human
 
Crossreactivity:Human Cytochrome c (100%), rat Cytochrome c (6.41%), horse Cytochrome c (0.92%), dog Cytochrome c (0.62%), bovine Cytochrome c (0.61%) and <0.03%: Bax, Bcl-2, Bcl-XL, Caspase-9, cIAP-1, Survivin
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 13, RIPA Cell lysis buffer 2, Digitonin cell permeabilization buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:Cytochrome c, which is a critical component of the electron transport chain, is a component of the functional apoptosome. Upon release from the mitochondria, cytochrome c binds to the adaptor proteins Apaf-1 and procaspase-9 to form a cytosolic apoptosome complex. This event results in activation of procaspase-9 and its subsequent cleavage and activation of caspase-3 leading to the degradation of a series of cellular substrates and subsequently cell death.
 
UniProt ID:P08574
 

Product Literature References

Gamma-tocotrienol acts as a BH3 mimetic to induce apoptosis in neuroblastoma SH-SY5Y cells: J.K. Tan, et al.; J. Nutr. Biochem. 31, 28 (2016), Application(s): Measured cytochrome c levels in the cytosol and mitochondria, Abstract;
Norcantharidin induces apoptosis in human prostate cancer cells through both intrinsic and extrinsic pathways: P.Y. Yang, et al.; Pharmacol. Rep. 68, 874 (2016), Application(s): Used to determine whether cytochrome c was released during norcantharidin-induced apoptosis, Abstract;
Butein shows cytotoxic effects and induces apoptosis in human ovarian cancer cells: P.Y. Yang, et al.; Am. J. Chin. Med. 43, 769 (2015), Abstract;
α-Terthienylmethanol, isolated from Eclipta prostrata, induces apoptosis by generating reactive oxygen species via NADPH oxidase in human endometrial cancer cells: J. S. Lee, et al.; J. Ethnopharmacol. 169, 426 (2015), Application(s): ELISA using human supernatant, Abstract;
Evidence of oxidative stress and mitochondrial respiratory chain dysfunction in an in vitro model of sepsis-induced kidney injury: C. Quoilin, et al.; Biochim. Biophys. Acta. 1837, 1790 (2014), Application(s): Quantification of cytochrome c in HK-2 cells, Abstract;
d-Penicillamine targets metastatic melanoma cells with induction of the unfolded protein response (UPR) and Noxa (PMAIP1)-dependent mitochondrial apoptosis: S. Qiao, et al.; Apoptosis 17, 1079 (2012), Application(s): EIA using metastatic melanoma cells, Abstract;

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