- Sensitive - detect picogram levels of protein compared to microgram levels in Western blot
- Reliable - thoroughly validated in-house with a variety of cell lines
- Efficient - save precious sample by using less per test
The Bax (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. The Bax (human), EIA kit provides sensitive results - detect picogram levels of protein compared to microgram levels in Western blot and extend the utility of your precious samples.
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Product Specification
| Alternative Name: | BCL2L4, BCL2-associated X protein |
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| Sensitivity: | 10.1 pg/ml (range 62.5 - 2,000 pg/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of Bax in cell lysates of human origin. |
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| Species reactivity: | Human
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| Crossreactivity: | Human Bax (100%), p21 (<0.7%) and <0.1%: Bcl-2, Bcl-XL, CDK4, p27, PCNA |
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| Use/Stability: | Store all components at +4°, except standard at -20°. |
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| Shipping: | Shipped on Blue Ice |
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| Kit/Set Contains: | Microtiter plate, Conjugate, Antibody, Assay buffer 13, Cell lysis buffer 4, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2 |
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| Scientific Background: | Bax is an appromimately 22 kDa pro-apoptotic protein belonging to the Bcl-2 family of proteins, which are characterized by the presence of at least one Bcl-2 homology (BH) domains. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes where it inserts and mediates the release of cytochrome c. This activates caspase-3 and perpetuates the apoptotic cascade. |
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| UniProt ID: | Q07812 |
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Product Literature References
Human blood molecular biodosimeter panel for distinguishing radiation exposure from inflammation stress: A.J. Wyrobek, et al.; (2016), (U. S. Patent),
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