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Bax (human), ELISA kit

ADI-900-138 96 wells 601.00 USD
Do you need bulk/larger quantities?
  • Sensitive - detect picogram levels of protein compared to microgram levels in Western blot
  • Reliable - thoroughly validated in-house with a variety of cell lines
  • Efficient - save precious sample by using less per test
The Bax (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. The Bax (human), EIA kit provides sensitive results - detect picogram levels of protein compared to microgram levels in Western blot and extend the utility of your precious samples.
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Product Specification

Alternative Name:BCL2L4, BCL2-associated X protein
Sensitivity:10.1 pg/ml (range 62.5 - 2,000 pg/ml)
Assay Time:3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of Bax in cell lysates of human origin.
Species reactivity:Human
Crossreactivity:Human Bax (100%), p21 (<0.7%) and <0.1%: Bcl-2, Bcl-XL, CDK4, p27, PCNA
Use/Stability:Store all components at +4°, except standard at -20°.
Shipping:Shipped on Blue Ice
Contents:Microtiter plate, Conjugate, Antibody, Assay buffer 13, Cell lysis buffer 4, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2
Scientific Background:Bax is an appromimately 22 kDa pro-apoptotic protein belonging to the Bcl-2 family of proteins, which are characterized by the presence of at least one Bcl-2 homology (BH) domains. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes where it inserts and mediates the release of cytochrome c. This activates caspase-3 and perpetuates the apoptotic cascade.
UniProt ID:Q07812

Product Literature References

Cilostazol Suppresses Aβ-induced Neurotoxicity in SH-SY5Y Cells through Inhibition of Oxidative Stress and MAPK Signaling Pathway: T. Oguchi, et al.; Front. Aging Neurosci. 9, 337 (2017), Application(s): SH-SY5Y cells lysates, Abstract; Full Text
Human blood molecular biodosimeter panel for distinguishing radiation exposure from inflammation stress: A.J. Wyrobek, et al.; (2016), (U. S. Patent), Full Text

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