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IL-1β (mouse), ELISA kit

Low price, sensitive (< 3pg/ml) IL-1β (mouse), ELISA kit for inflammation and cytokine research.
 
ADI-900-132A 96 wells 530.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ADI-900-132

  • Rapid – results in just 4 hours from up to 42 samples in duplicate
  • Sensitive – reliably measure < 3pg/ml of mouse IL-1β
  • Specific – low cross-reactivity with related cytokines
  • Ready-to-use – reagents and pre-coated plates reduce errors and save your time
The IL-1β (mouse), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4 hours.
900-132A std curve
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900-132A std curve

Product Specification

Alternative Name:Interleukin-1β
 
Sensitivity:< 3 pg/ml (range 31.25 - 1,000 pg/ml)
 
Assay Time:4 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of IL-1β in culture supernatants, plasma, and serum of mouse origin.
 
Species reactivity:Mouse
 
Crossreactivity:Cross reactivities: mouse IL-1β (100%). Unaffected by mouse IL-1α, IL-2, IL-4, IL-6, IL-10, TNF-α, and GM-CSF; human IL-1α and IL-1β, IL-8; or bovine IL-1β. Does not cross react with rat IL-1β (12%).
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:Microtiter plate, SA-HRP concentrate, SA-HRP dilution buffer, Biotinylated antibody, Standard, Standard diluent, Wash buffer concentrate, TMB Substrate, Stop solution
 
Scientific Background:The interleukin-1 (IL-1) cytokine family consists of IL-1α, IL-1β, and IL-1Rα (receptor antagonist) which are expressed primarily by endothelial cells and activated macrophages. IL-1α and IL-1β elicit pro-inflammatory cytokine production and cellular activation, and are the primary mediators of hypothalamic regulation that results in fever. IL-1α and IL-1β signals are transmitted via a dual subunit IL-1 receptor composed of IL-1R1 and the IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP is vital to IL-1 signaling, as it recruits the IL-1 receptor-associated kinase (IRAK) to the receptor complex, initiating NF-κB pathway activation.
 
UniProt ID:P10749
 

Product Literature References

Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages: Y.A. Bae, et al.; Korean J. Physiol. Pharmacol. 20, 415 (2016), Application(s): ELISA using culture supernatants, Abstract; Full Text
CLOCK modulates survival and acute lung injury in mice with polymicrobial sepsis: C.Y. Wang, et al.; Biochem. Biophys. Res. Commun. 478, 935 (2016), Application(s): Measurement of cytokine levels in serum and cell culture medium, Abstract;
Antioxidative, anti-inflammatory, and matrix metalloproteinase inhibitory activities of 20 (S)-ginsenoside Rg3 in cultured mammalian cell lines: Y.M. Shin, et al.; Mol. Biol. Rep. 40, 269 (2013), Application(s): EIA using culture supernatants from RAW264.7 macrophage cells, Abstract;
Cilostazol enhances apoptosis of synovial cells from rheumatoid arthritis patients with inhibition of cytokine formation via Nrf2-linked heme oxygenase 1 induction: S.Y. Park, et al.; Arthritis Rheum. 62, 732 (2010), Application(s): EIA using mouse serum and cell culture supernatant, Abstract; Full Text
Mitogen-activated protein kinase phosphatase-1 deficiency decreases atherosclerosis in apolipoprotein E null mice by reducing monocyte chemoattractant protein-1 levels: S. Imaizumi, et al.; Mol. Genet. Metab. 101, 66 (2010), Application(s): EIA using mouse serum, Abstract; Full Text

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