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IL-1β (human), ELISA kit

 
ADI-900-130A 96 wells 546.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ADI-900-130

  • Sensitive - quantify down to 1 pg/mL
  • Efficient - only 50 µl diluted sample required saving precious materials
  • Specific - no cross-reactivity with related cytokines
  • Results from up to 40 samples in duplicate in just 3 hours
  • Ready-to-use reagents and pre-coated plates reduce errors and save your time
The IL-1β (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 450 nm. Quantify 1 pg/mL IL-1β with lot sample volume needed saving you precious sample.

Product Specification

Alternative Name:Interleukin-1β
 
Sensitivity:1 pg/mL
 
Assay Time:< 3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of IL-1β in culture supernatant, plasma, serum, and urine of human origin.
 
Species reactivity:Human
 
Crossreactivity:The following substances were tested and found to have no cross-reactivity: human IL-1α, IL-1ra, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, IFN-α, TGF-β, GM-CSF, G-CSF, OSM, MIP-1α, MIP-1β, LIF, MCP-1, G-CSF, RANTES, PF-4, βTG, GRO, IP-10, SCF, TNF-α, and TNF-β.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:+4°C
 
Kit/Set Contains:Standard, Standard Diluent Buffer, Coated 96-well plate, Conjugate, Streptavidin-HRP, Streptavidin-HRP Diluent, Wash Buffer Concentrate, TMB Solution, Stop Solution, Plate Sealers
 
Scientific Background:The interleukin-1 (IL-1) cytokine family consists of IL-1α, IL-1β, and IL-1Rα (receptor antagonist) which are expressed primarily by endothelial cells and activated macrophages. IL-1α and IL-1β elicit pro-inflammatory cytokine production and cellular activation, and are the primary mediators of hypothalamic regulation that results in fever. IL-1α and IL-1βsignals are transmitted via a dual subunit IL-1 receptor composed of IL-1R1 and the IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP is vital to IL-1 signaling, as it recruits the IL-1 receptor-associated kinase (IRAK) to the receptor complex, initiating NF-κB pathway activation.
 
UniProt ID:P01584
 

Product Literature References

Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci. 161, 37 (2016), Application(s): Assessment of the inhibitory effect of VD2 on the production of pro-inflammatory cytokines, Abstract;

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