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XIAP (human), ELISA kit

 
ADI-900-124 96 wells 494.00 USD
Do you need bulk/larger quantities?
 
  • Convenient - pre-coated plates and liquid color-coded reagents reduce errors
  • Quantitative - save time and achieve fully quantitative results compared to semi-quantitative Western blot analysis
  • Specific - no reactivity with structurally related molecules
The XIAP (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
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Product Specification

Alternative Name:X-linked inhibitor of apoptosis
 
Sensitivity:90.6 pg/ml (range 312.5 - 10,000 pg/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of human XIAP in cell lysates.
 
Species reactivity:Human
 
Crossreactivity:Human XIAP (100%), Survivin (3.2%) and <0.1%: cIAP-1, Caspase-3, Smac, DIABLO
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 21, RIPA Cell lysis buffer 2, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2
 
Scientific Background:XIAP (X-linked inhibitor of apoptosis) belongs to the IAP (inhibitors of apoptosis) family, which is characterized by one or more BIR (baculovirus IAP repeat) domains. These proteins have multiple biological functions that include binding and inhibiting caspases, regulating cell cycle progression, and modulating receptor-mediated signal transduction. XIAP functions by direct binding and inhibition of several caspases, including caspase-3, caspase-7 and caspase-9.
 
UniProt ID:P98170
 

Product Literature References

The flavonolignan silibinin potentiates TRAIL-induced apoptosis in human colon adenocarcinoma and in derived TRAIL-resistant metastatic cells: H. Kauntz, et al.; Apoptosis 17, 797 (2012), Application(s): ELISA using cell lysates, Abstract;

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