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MEK1 ELISA kit

 
ADI-900-122A 96 wells 483.00 USD
Do you need bulk/larger quantities?
 
  • Sensitive - use less precious sample per test compared to Western blot
  • Higher throughput - test up to 40 samples in duplicate in just 3 hours
  • Quantitative - obtain statistically signficant results and compare data between multiple experiments
  • Ready-to-use - save time and reduce errors with pre-coated plates
The MEK1 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours that measures pan MEK1. Absorbance is read at 450 nm.

Product Specification

Alternative Name:MAPK2K1, MAPKK1, MAP kinase kinase 1, MAPK/ERK kinase 1
 
Sensitivity:139.0 pg/ml (range 312.5 - 10,000 pg/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of MEK1 in cell lysates of human and rat origin.
 
Species reactivity:Human, Rat
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 21, Cell lysis buffer 3, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2
 
Scientific Background:MEK1 is also known by a variety of other names. They include dual specificity mitogen-activated protein kinase kinase (MAP kinase kinase 1 or MAPKK 1), ERK activator kinase 1, MAPK/ERK kinase 1, ERK kinase 1 and MAP kinase kinase. MEK1 is a 393 amino acid, 43.5kD protein that is highly conserved in evolution. MEK phosphorylates threonine and tyrosine residues on MAP kinases ERK 1 and 2 (p44 and p42 MAP kinase). MEK participates in a wide range of cellular processes including cell proliferation, differentiation and apoptosis. MEK1 is activated by phosphorylation of Ser218 and Ser222 by the serine-threonine kinase RAF1, which is part of the p21ras signal transduction pathway. Constitutive activation of MEK1 results in cellular transformation. This protein kinase has been reported to be a likely target for pharmacological intervention in proliferative diseases.
 
UniProt ID:Q02750 (human)
 

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