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IgM (mouse), ELISA kit

 
ADI-900-120 96 wells 342.00 USD
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  • Time savings - determine the isotype and concentration of monoclonal antibodies
  • Quantitative - suitable in determining concentration
  • Specific - no reactivity with other immunoglobulins
  • Fast - results from up to 40 samples in duplicate in just 90 minutes
The IgM (mouse), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 90 minutes. Absorbance is read at 450 nm. Ideal for determining the isotype and concentration of monoclonal antibodies.
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Product Specification

Sensitivity:0.60 ng/ml (range 3.91 - 250 ng/ml)
 
Assay Time:90 minutes
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of IgM in ascites fluid, culture supernatants, and serum of mouse origin.
 
Species reactivity:Mouse
 
Crossreactivity:Mouse IgM (100%), rat IgM (60%), human IgM (<0.4%), mouse IgG1 (<0.2%) and <0.10%: mouse IgG2a, mouse IgG2b, mouse IgG3
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:GxM IgG Microtiter plate, mouse IgM conjugate, Assay buffer 13 concentrate, Wash buffer concentrate, mouse IgGM standard, TMB Substrate, Stop solution 2
 
Scientific Background:Immunoglobulin M (IgM) is an immunoglobulin expressed initially on the surface of B-cells as a monomer of two mu heavy chains and two light chains. Following B-cell activation, IgM is secreted in its pentameric form, consisting of five IgM molecules joined at the base of the Fc domain by a J chain. IgM is the first Immunoglobulin expressed following infection.
 

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