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[pSer218/Ser222]MEK1 ELISA kit

 
ADI-900-119 96 wells 510.00 USD
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  • Quantitative - fully quantitative results surpass semi-quantitative Western blot analysis
  • Specific - only reacts with phosphorylated MEK1
  • Higher throughput - assay up to 40 samples in duplicate in just 3 hours
The [pSer218/Ser222]MEK1 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
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Product Specification

Alternative Name:MAPK2K1, MAPKK1, MAP kinase kinase 1, MAPK/ERK kinase 1
 
Sensitivity:85.2 pg/ml (range 187.5 - 6,000 pg/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of [pSer218/Ser222]MEK1 in cell lysates of human, mouse, and rat origin.
 
Species reactivity:Human, Mouse, Rat
 
Crossreactivity:pMEK 1 (100%), pMEK 2 (12.2%) and <0.1%: MEK-1 (inactive), phospho Jnk, Erk2, phospho Erk2
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 22 concentrate, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2
 
Scientific Background:MEK is a dual specificity kinase capable of phosphorylating both tyrosine and threonine residues. The MEK family, which is also known as MAP kinase kinase, phosphorylates MAP kinases on the conserved T-X-Y motif; phosphorylation of MAPK by MEK results in an increase in MAPK activity. MEK is involved in a diverse array of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis.
 
UniProt ID:Q02750 (human)
 

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