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Myeloperoxidase (human), ELISA kit

 
ADI-900-115 96 wells 447.00 USD
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  • Sensitive - detect as little as 19 pg/ml MPO
  • Reliable - excellent reproducibility and parallelism
  • Convenient - ready-to-use liquid color-coded reagents reduce error and save time in the lab
  • Higher throughput - results form up to 39 sampels in duplicate in just 3 hours
The Myeloperoxidase (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Tihs sensitive kit lets you quantitate as little as 19 pg/mL MPO in a wide variety of sample matrices that have been validated in-house. Convenient ready-to-use liquid color-coded reagents reduce error and save time in the lab.
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Product Specification

Alternative Name:MPO
 
Sensitivity:0.019 ng/ml (range 0.195 - 12.5 ng/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of human Myeloperoxidase of culture supernatants, nasal lavage, Lithium-heparin plasma, sputum supernatant, and urine.
 
Species reactivity:Human
 
Crossreactivity:Human Myeloperoxidase (100%), human Erythropoietin (1.1%), bovine Lactoperoxidase (0.40%), human Elastase (0.20%), human Lactoferrin (0.20%), sheep COX-2 (0.10%) and <0.10%: human Thyroid Peroxidase, human Eosinophil-derived Neurotoxin
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 13, Assay buffer 31 concentrate, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2
 
Scientific Background:Myeloperoxidase (MPO) is synthesized as a single chain precursor, which is then processed into a tetramer consisting of 2 light chains and 2 heavy chains. In response to microbial invasion, MPO is released from the cytoplasmic granules of neutrophils into the extracellular space and phagosome, where it generates hypohalous acids that have a strong microbicidal effect. Myeloperoxidase can be used as a marker of airway inflammation caused either by disease, such as asthma, or by environmental irritants.
 
UniProt ID:P05164
 

Product Literature References

The AIM2 inflammasome is a central regulator of intestinal homeostasis through the IL-18/IL-22/STAT3 pathway: R.A. Ratsimandresy, et al.; Cell. Mol. Immunol. 14, 127 (2017), Application(s): MPO activity, colon homogenates, Abstract; Full Text
Usefulness of Plasma Matrix Metalloproteinase-3 (MMP-3) Levels to Predict Myocardial Infarction in Men with and without Acute Coronary Syndrome: E. Cavusoglu, et al.; Am. J. Cardiol. 117, 881 (2016), Application(s): ELISA measuring plasma levels of myeloperoxidase, Abstract;

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