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13(S)-HODE ELISA kit

 
ADI-900-108 96 wells 404.00 USD
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  • Specific - negligible reactivity with related molecules
  • Simple- ready-to-use liquid color-coded reagents reduce error
  • Trusted - cited in peer-reviewed publications
  • Reliable - reproducible results day-after-day and lot-after-lot
The 13(S)-HODE EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 4 hours. Absorbance is read at 405 nm. The kit provides ready-to-use non-radioactive liquid color-coded reagents that reduce error in the lab.
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Product Specification

Alternative Name:13-hydroxyoctadecadienoic acid
 
Sensitivity:1.6 ng/ml (range 3.9 - 1,000 ng/ml)
 
Assay Time:4 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of 13(S)-HODE in culture supernatants, cell lysates, serum, saliva, tissue, and urine from any species. Cited sample type includes plasma (ref. 1).
 
Species reactivity:Species independent
 
Use/Stability:Store all components +4°, except conjugate -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:GxR IgG Microtiter plate, Assay buffer, Conjugate, Antibody, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:The major human dietary poly-unsaturated fatty acid is linoleic acid, an essential fatty acid that can be metabolized to either 13-hydroxyoctadecadienoic acid [13(S)-HODE] via lipoxygenase or [13(R)-HODE] by p450 enzymes. Alternatively, cyclooxygenase metabolism of linoleic acid results in the production of hydroxyeicosatetraenoic acid [15(S)-HETE]. Changes in the intra-cellular ratio of 13(S)-HODE to 15(S)-HETE have been linked to a number of physiological conditions including cancer and cardiovascular disease. 13(S)-HODE helps to mediate cell adhesion and excessive proliferation that is critical in the formation of tumors and metastases, as well as the formation of atherogenic plaques.
 

Product Literature References

The oxidative stress mediator 4-hydroxynonenal is an intracellular agonist of the nuclear receptor peroxisome proliferator-activated receptor-β/δ (PPARβ/δ): K. Prabhu, et al. ; Free Radic. Biol. Med. 42, 1155 (2007), Application(s): EIA using human plasma (ref. 1), Abstract;
The transcription factor GATA-6 is overexpressed in vivo and contributes to silencing 15-LOX-1 in vitro in human colon cancer: I. Shureiqi, et al. ; FASEB J. 21, 743 (2007), Application(s): EIA using human cell lysates & culture supernatants, Abstract;

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