Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

[pThr180/Tyr182]p38 ELISA kit

 
ADI-900-101 96 wells 594.00 USD
Do you need bulk/larger quantities?
 
  • Sensitive - use less precious sample per test compared to Western blot
  • Higher throughput - test up to 40 samples in duplicate in just 3 hours
  • Quantitative - obtain statistically signficant results and compare data between multiple experiments
  • Ready-to-use - save time and reduce errors with pre-coated plates
The [pThr180/Tyr182]p38 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. Use less precious sample per test compared to Western blot with higher throughput sample analysis; measure up to 40 samples in duplicate per kit.
900-101-Kit-Graph
Please mouse over
900-101-Kit-Graph

Product Specification

Alternative Name:SAPK2, SAP kinase 2
 
Sensitivity:52.1 pg/ml (range 156 - 5,000 pg/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of [pThr180/Tyr182]p38 in cell lysates of human and mouse origin.
 
Species reactivity:Human, Mouse
 
Crossreactivity:Phospho p38 (100%) and <0.01%: non-phosphorylated p38, phospho Jnk, non-phosphorylated Jnk, AKT, non-phosphorylated Erk, phospho Erk
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 4 concentrate, Wash buffer concentrate, Standard, TMB Substrate, RIPA Cell lysis buffer 2, Stop solution 2
 
Scientific Background:Six proteins of the p38 subfamily of MAP kinases have been identified to date, including: p38α (SAPK2, CSBP) and its splice variant Mxi; p38β and β2; p38γ (ERK6, SAPK3); and p38δ (SAPK4). p38 MAP kinases contain a conserved TGY motif in their activation loop and are activated in response to cytokines, hormones, GPCRs, and osmotic and heat shock to mediate primarily pro-apoptotic and pro-inflammatory responses. MKK3, MKK4, and MKK6 are known to activate p38 family members with varying specificity. All four p38 isoforms are able to phosphorylate the activating transcription factor 2 (ATF2), and differentially activate numerous substrates including Mnk1/2, Msk, phospholipase A2, and the MAPKAP kinase-2 and -3. Activated MAPKAP kinase-2 subsequently phosphorylates the heat shock protein Hsp27, an F-actin binding protein involved in cytoskeletal maintenance.
 
UniProt ID:Q16539 (human p38α), Q15759 (human p38β), P53778 (p38γ), O15264 (p38δ)
 

Product Literature References

Intestinal anti-inflammatory activity of the seeds of Raphanus sativus L. in experimental ulcerative colitis models: K.C. Choi, et al. ; J. Ethnopharmacol. 179, 55 (2016), Application(s): Measurement of p-p38 kinase in rat whole cell lysates, Abstract;
Catechin-7-O-β-D-glucopyranoside isolated from the seed of Phaseolus calcaratus Roxburgh ameliorates experimental colitis in rats: S.H. Kook, et al.; Int. Immunopharmacol. 29, 521 (2015), Application(s): ELISA measuring enzymatic activity of p38 kinase in rat tissue, Abstract;

Product Toolbox

PRODUCT RESOURCES

Datasheet
Assay Layout Sheet
MSDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By target:
p38
By biological activity:
p38 Immunoassay kit
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Catalogs
Immunoassay Kits
Immunoassay Kits
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,