[pThr²⁰²/Tyr²⁰⁴]Erk1/2 ELISA kit

Sensitive ELISA kit for this key cell signaling molecule for kinase, proliferation, apoptosis, and differentiation research.



ADI-900-098A-0001	96 wells	590.00 USD

Replaces Prod. #: ADI-900-098

Sensitive - measure as little as 2.67 pg/ml of pERK
Higher throughput - test up to 40 samples in duplicate in just 3 hours
Quantitative - obtain statistically significant results and compare data between multiple experiments
Ready-to-use - save time and reduce errors with pre-coated plates

The [pThr²⁰²/Tyr²⁰⁴]Erk1/2 EIA kit is a complete assay for the quantitative determination of phosphorylated Erk1/2 using colorimetric detection read at 450 nm. The Extracellular regulated kinases, Erk-1 and Erk-2, both play a critical role in cellular proliferation. These extracellular kinases, are known to reside downstream of the Mitogen activated protein kinase/Extracellular protein kinases referred to as MEK. Both MEK-1 and MEK-2 phosphorylate and activate Erk on the conserved TEY motif within the activation loop of the kinase.

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Product Specification

Sensitivity:	2.67 pg/ml (range 62.5 - 2,000 pg/ml)

Assay Time:	3 hours

Application:	For the measurement of [pThr²⁰²/Tyr²⁰⁴]Erk1/2 in cell lysates of human, mouse, and rat origin.

Crossreactivity:	Phospho Erk (100%) and <0.01%: non-phosphorylated Erk, phospho p38, non-phosphorylated p38, phospho Jnk, non-phosphorylated Jnk, phospho AKT and non-phosphorylated AKT

Use/Stability:	Store all components at +4°, except standard at -20°.

Kit/Set Contains:	Microtiter plate, Conjugate, Antibody, Assay buffer 21, Wash buffer concentrate, Standard, TMB Substrate, RIPA Cell lysis buffer 2, Stop solution 2

Miscellaneous/General:	The mitogen-activated protein kinase (MAPK) pathway exists in all eukaryotes. It consists of several subgroups including ERK 1/2 (extracellular signal-regulated kinase), JNK and p38 kinases. These kinases regulate and control fundamental cellular processes including proliferation, apoptosis, survival and differentiation. They are activated by diverse stimuli including cytokines, growth factors, irradiation, changes in osmolarity, heat and shear stress. MAP kinases are characterized by their requirement for dual phosphorylation for full activation. ERK1 (44kDa) and ERK2 (42kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif. The literature contains numerous and extensive reviews on MAP kinase.

Product Literature References

Autocrine fibroblast growth factor 2-mediated interactions between human mesenchymal stem cells and the extracellular matrix under varying oxygen tension: J. Kim, et al.; J. Cell Biochem 114, 716 (2013), Abstract;

Activation of the extracellular signal-regulated kinases 1 and 2 (ERK1/2) is needed for the TGFβ-induced chondrogenic and osteogenic differentiation of mesenchymal stem cells: N.A. Arita, et al.; Biochem. Biophys. Res. Commun. 405, 564 (2011), Application(s): EIA using human cell lysates, Abstract; Full Text

Involvement of ERK-1/2 in IL-21-induced cytokine production in leukemia cells and human monocytes: S. Adunyah, et al. ; Cytokine 44, 101 (2008), Application(s): EIA using human cell lysates, Abstract;

The Ras/Raf-1/MEK1/ERK Signaling Pathway Coupled to Integrin Expression Mediates Cholinergic Regulation of Keratinocyte Directional Migration: A.I. Chernyavsky, et al. ; J. Biol. Chem. 280, 39220 (2005), Application(s): EIA using human cell lysates, Abstract;

Transcriptional Regulation of Activating Transcription Factor 3 Involves the Early Growth Response-1 Gene: F.G. Bottone, et al. ; J. Pharmacol. Exp. Ther. 315, 668 (2005), Application(s): EIA using human cell lysates, Abstract;

Secretin Activation of Chromogranin A Gene Transcription: IDENTIFICATION OF THE SIGNALING PATHWAYS IN CIS AND IN TRANS: N.R. Mahapatra, et al. ; J. Biol. Chem. 278, 19986 (2003), Application(s): EIA using rat cell lysates, Abstract;