Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Corticosterone ELISA kit

Widely used sensitive corticosterone ELISA kit with a large variety of sample types and highly reproducible results for animal stress research.
 
ADI-900-097 96 wells 320.00 USD
 
Alternative size available: ADI-901-097 (5x96 wells)
  • Higher throughput - results from up to 39 samples in duplicate in just 3 hours
  • Trusted - routinely used to monitor stress levels in zoo animals
  • Flexible - broad dynamic range suitable for multiple sample matrices
The Corticosterone EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. The Corticosterone EIA kit has low cross-reactivity with related steroids. The broad dynamic range makes this kit ideal for a wide variety of sample matrices from any species.
900-097-Kit-Graph
Please mouse over
900-097-Kit-Graph

Product Specification

Sensitivity:27.0 pg/ml (range 32 - 20,000 pg/ml)
 
Assay Time:3 hours
 
Application:For the quantitative determination of Corticosterone in culture supernatants, feces, plasma, serum, and saliva from any species. Cited sample types include cell lysate, urine and whole blood.
 
Crossreactivity:Corticosterone (100%), Deoxycorticosterone (21.3%), Desoxycorticosterone (21.0%), Progesterone (0.46%), Testosterone (0.31%), Tetrahydrocorticosterone (0.28%), Aldosterone (0.18%), Cortisol (0.046%) and <0.03%: Pregnenolone, Estradiol, Cortisone, 11-dehydrocorticosterone acetate
 
Long Term Storage:+4°C
 
Kit/Set Contains:DxS IgG Microtiter plate, Assay buffer 15 concentrate, Steroid displacement reagent, Conjugate, Antibody, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Miscellaneous/General:Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to the stimulation of the adrenal cortex by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Corticosterone production has been shown to increase with stress, with elevated corticosterone levels being associated with impairment of long term memory retrieval and following burn injury. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns.
 
Protocol:Suggested Protocol for Small Volumes of Serum/Plasma Samples
  1. Let all solutions come to room temp before use.
  2. Aliquot 10µl of each sample into microfuge tube.
  3. Make 1ml 1:100 Steroid Displacement Reagent (SDR) solution (immediately before use) in deionized water or PBS (not assay buffer)
  4. Add 10µl 1:100 SDR to each sample tube.
  5. Vortex, let stand >5 minutes before diluting with EIA buffer.
  6. Add 380µl EIA assay buffer to each plasma tube, vortex.
Final dilution is ~1:40 (This final dilution may not be appropriate for every sample; thus, optimal dilution must be determined by each end user for their experimental sample group.)
 

Product Literature References

Sex differences and similarities in the neuroendocrine regulation of social behavior in an African cichlid fish: L.A. O'Connell, et al.; Horm. Behav. 64, 468 (2013), Application(s): EIA using fish plasma, Abstract;
Comparative patterns of adrenal activity in captive and wild Canada lynx (Lynx canadensis) : K.V. Fanson, et al.; J. Comp. Physiol. B. 182, 157 (2012), Application(s): EIA using lynx feces, Abstract;
Environmental enrichment during rearing alters corticosterone levels, thymocyte numbers, and aggression in female BALB/c mice: E.K. Hutchinson, et al.; J. Am. Assoc. Lab. Anim. 51, 18 (2012), Application(s): ELISA using mouse urine, Abstract; Full Text
Oral gavage in rats: animal welfare evaluation: P.V. Turner, et al.; J. Am. Assoc. Lab. Anim. Sci. 51, 25 (2012), Application(s): EIA using rat feces, Abstract; Full Text
Periodic maternal separation decreases hippocampal neurogenesis without affecting basal corticosterone during the stress hyporesponsive period, but alters HPA axis and coping behavior in adulthood: N. Lajud, et al.; Psychoneuroendocrinology 37, 410 (2012), Application(s): Corticosterone levels in rat plasma, Abstract;
Role of the pituitary–adrenal axis in granulocyte-colony stimulating factor-induced neuroprotection against hypoxia–ischemia in neonatal rats: M.S. Charles, et al.; Neurobiol. Dis. 47, 29 (2012), Application(s): ELISA using rat plasma, Abstract;
The CRF1 receptor antagonist SSR125543 attenuates long-term cognitive deficit induced by acute inescapable stress in mice, independently from the hypothalamic pituitary adrenal axis: J. Philbert, et al.; Pharmacol. Biochem. Behav. 102, 415 (2012), Application(s): ELISA using mouse plasma, Abstract;
Effects of short-term fasting in male Sprague-Dawley rats: M.H. Nowland, et al.; Comp. Med. 61, 138 (2011), Application(s): Rat serum corticosterone, Abstract; Full Text
Pumilio-2 function in the mouse nervous system: H. Siemen, et al.; PLoS One 6, e25932 (2011), Application(s): Feces and serum corticosterone, Abstract; Full Text
Restraint stress and repeated corticotrophin-releasing factor receptor activation in the amygdala both increase amyloid-beta precursor protein and amyloid-beta peptide but have divergent effects on brain-derived neurotrophic factor and pre-synaptic proteins in the prefrontal cortex of rats: Ray, et al.; Neuroscience 184, 139 (2011),
Strain differences in the neurochemical response to chronic restraint stress in the rat: Relevance to depression: C.M. O'Mahony, et al.; Pharmacol. Biochem. Behav. 97, 690 (2011), Application(s): Rat plasma corticosterone, Abstract;
Stress and glucocorticoids regulated corticotropin releasing factor in rat prefrontal cortex: Q.Y. Meng, et al.; Mol. Cell. Endocrinol. 342, 54 (2011), Application(s): Corticosterone levels in rat plasma, Abstract;
Alcohol extract of Echinacea pallida reverses stress-delayed wound healing in mice: Z. Zhai, et al.; Phytomedicine 16, 669 (2009), Application(s): Mouse plasma corticosterone , Abstract; Full Text
Dietary sodium manipulation during critical periods in development sensitize adult offspring to amphetamines: F. Flynn, et al. ; Am. J. Physiol. Regul. Integr. Comp. Physiol. 295, R899 (2008), Application(s): EIA using rat plasma, Abstract;
Lack of adrenomedullin in the mouse brain results in behavioral changes, anxiety, and lower survival under stress conditions: A. Martinez, et al. ; PNAS 105, 12581 (2008), Application(s): EIA using mouse serum, Abstract;
MCP-1-deficient mice show reduced neuroinflammatory responses and increased peripheral inflammatory responses to peripheral endotoxin insult: L. Van, et al.; J. Neuroinflammation 5, U1 (2008), Application(s): EIA using mouse serum, Abstract; Full Text
The probiotic Bifidobacteria infantis: An assessment of potential antidepressant properties in the rat: L. Desbonnet, et al. ; J. Psychiatr. Res. 43, 164 (2008), Application(s): EIA using rat plasma, Abstract;
Streptozotocin induces G(2) arrest in skeletal muscle myoblasts and impairs muscle growth in vivo: T. Hawke, et al. ; Am. J. Physiol., Cell Physiol. 292, C1033 (2007), Application(s): EIA using rat serum, Abstract;
The anorexic effect of alpha-melanocyte-stimulating hormone ismediated by corticotrophin-releasing factor in chicks: T. Tachibana, et al. ; Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 147, 173 (2007), Application(s): EIA using avian plasma, Abstract;
Leptin Corrects Host Defense Defects after Acute Starvation in Murine Pneumococcal Pneumonia : P. Mancuso, et al.; Am. J. Respir. Crit. Care Med. 173, 212 (2006), Application(s): ELISA using mouse whole blood, Abstract; Full Text
CRH stimulation of corticosteroids production in melanocytes is mediated by ACTH: A. Slominski, et al. ; Am. J. Physiol. Endocrinol. Metab. 288, E701 (2005), Application(s): EIA using human culture supernatants, Abstract;
Early life immune challenge alters innate immune responses to lipopolysaccharide: implications for host defense as adults: S. Ellis, et al. ; FASEB J. 19, 1519 (2005), Application(s): EIA using rat plasma, Abstract;
Hypertension from targeted ablation of chromogranin A can be rescued by the human ortholog: N.R. Mahapatra, et al. ; J. Clin. Invest. 115, 1942 (2005), Application(s): EIA using mouse cell lysates, Abstract;
Stress hormones: a link between maternal condition and sex-biased reproductive investment: O.P. Love, et al.; Am. Nat. 166, 751 (2005), Application(s): EIA using avian plasma, Abstract;
Urinary corticosterone measures: effects of strain and social rank in BKW and CD-1 mice: A.E. Fitchett, et al.; Behav. Processes 70, 168 (2005), Application(s): ELISA using mouse urine, Abstract;

Related Literature

Catalogs
Immunoassay Kits
Immunoassay Kits
25-Jun-14
Download as PDF

All new literature pieces

Recommend this page

 
Keep in touch

©2014 Enzo Life Sciences, Inc.,