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Corticosterone ELISA kit

Highly sensitive corticosterone ELISA kit, species independent, used for animal stress research.
ADI-900-097 96 wells 325.00 USD
Alternative size available: ADI-901-097 (5x96 wells)
  • Higher throughput with results from up to 39 samples in duplicate in just 3 hours
  • Flexibility built-in, measure corticosterone in any species
  • Trusted and routinely used to monitor stress levels in zoo animals
  • Broad dynamic range suitable for multiple sample matrices
The Corticosterone EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. The Corticosterone EIA kit has low cross-reactivity with related steroids. The broad dynamic range makes this kit ideal for a wide variety of sample matrices from any species.
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Product Specification

Sensitivity:27.0 pg/ml (range 32 - 20,000 pg/ml)
Assay Time:3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of Corticosterone in culture supernatants, feces, plasma, serum, and saliva from any species. Cited sample types include cell lysate, urine and whole blood.
Species reactivity:Species independent
Crossreactivity:Corticosterone (100%), Deoxycorticosterone (21.3%), Desoxycorticosterone (21.0%), Progesterone (0.46%), Testosterone (0.31%), Tetrahydrocorticosterone (0.28%), Aldosterone (0.18%), Cortisol (0.046%) and <0.03%: Pregnenolone, Estradiol, Cortisone, 11-dehydrocorticosterone acetate
Long Term Storage:+4°C
Kit/Set Contains:DxS IgG Microtiter plate, Assay buffer 15 concentrate, Steroid displacement reagent, Conjugate, Antibody, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Scientific Background:Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to the stimulation of the adrenal cortex by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Corticosterone production has been shown to increase with stress, with elevated corticosterone levels being associated with impairment of long term memory retrieval and following burn injury. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns.
Protocol:Suggested Protocol for Small Volumes of Serum/Plasma Samples
  1. Let all solutions come to room temp before use.
  2. Aliquot 10µl of each sample into microfuge tube.
  3. Make 1ml 1:100 Steroid Displacement Reagent (SDR) solution (immediately before use) in deionized water or PBS (not assay buffer)
  4. Add 10µl 1:100 SDR to each sample tube.
  5. Vortex, let stand >5 minutes before diluting with EIA buffer.
  6. Add 380µl EIA assay buffer to each plasma tube, vortex.
Final dilution is ~1:40 (This final dilution may not be appropriate for every sample; thus, optimal dilution must be determined by each end user for their experimental sample group.)

Product Literature References

Acute isoproterenol induces anxiety-like behavior in rats and increases plasma content of extracellular vesicles: G. Leo, et al.; Physiol. Behav. 142C, 79 (2015), Application(s): ELISA, Abstract;
Bifidobacteria Modulate Cognitive Processes in an Anxious Mouse Strain: H.M. Savignac, et al.; Behav. Brain Res. (2015), Application(s): ELISA using mouse plasma, Abstract;
Determination of PCNA, cyclin D3, p27, p57 and apoptosis rate in normal and dexamethasone-induced intrauterine growth restricted rat placentas: H. Er, et al.; Acta. Histochem. (2015), Application(s): ELISA using rat plasma, Abstract;
Early life environmental and pharmacological stressors result in persistent dysregulations of the serotonergic system: P. Wong, et al.; Front. Behav. Neurosci. 9, 94 (2015), Application(s): ELISA using mouse serum, Abstract; Full Text
Effects of developmental alcohol exposure vs. intubation stress on BDNF and TrkB expression in the hippocampus and frontal cortex of neonatal rats: K. E. Boschen, et al.; Int. J. Dev. Neurosci. 43, 16 (2015), Application(s): ELISA using rat supernatant, Abstract;
Enhanced Corticosteroid Signaling Alters Synaptic Plasticity in the Dentate Gyrus in Mice Lacking the Fragile X Mental Retardation Protein: M. Ghilan, et al.; Neurobiol. Dis. (2015), Application(s): ELISA using serum isolated from mouse trunk blood, Abstract;
Food, stress, and circulating testosterone: Cue integration by the testes, not the brain, in male zebra finches (Taeniopygia guttata): S. E. Lynn, et al.; Gen. Comp. Endocrinol. 215, 1 (2015), Application(s): ELISA using zebra finch plasma , Abstract;
Granulocyte-colony stimulating factor activates JAK2/PI3K/PDE3B pathway to inhibit corticosterone synthesis in a neonatal hypoxic-ischemic brain injury rat model: M. S. Charles, et al.; Exp. Neurol. (2015), Application(s): ELISA using rat growth media, Abstract;
Gut microbiota depletion from early adolescence in mice: Implications for brain and behaviour: L. Desbonnet, et al.; Brain Behav. Immun. (2015), Application(s): ELISA using mouse serum, Abstract;
Hyper-responsivity to stress in rats is associated with a large increase in amygdala volume. A 7 T MRI study: J. Bourgin, et al.; Eur. Neuropsychopharmacol. (2015), Application(s): ELISA using rat plasma, Abstract;
Lack of seasonal and moult-related stress modulation in an opportunistically breeding bird: the white-plumed honeyeater (Lichenostomus penicillatus): W.A. Buttemer, et al.; Horm. Behav. (2015), Application(s): ELISA using avian plasma, Abstract;
Lack of stress responses to long-term effects of corticosterone in Caps2 knockout mice: Y. Mishima, et al.; Sci. Rep. 5, 8932 (2015), Application(s): ELISA using mouse serum, Abstract; Full Text
N-acetylcysteine prevents behavioral and biochemical changes induced by alcohol cessation in rats: R. Schneider Jr, et al.; Alcohol 49, 259 (2015), Application(s): ELISA, Abstract;
NLRP3 Inflammasome Mediates Chronic Mild Stress-induced Depression in Mice via Neuroinflammation: Y. Zhang, et al.; Int. J. Neuropsychopharmacol. (2015), Application(s): ELISA, Abstract; Full Text
Predictable enriched environment prevents development of hyperemotionality in the VPA rat model of autism: M. R. Favre, et al.; Front. Neurosci. (2015), Application(s): ELISA using rat plasma, Abstract;
Serotonin 2 C receptor contributes to gender differences in stress-induced hypophagia in aged mice: C. Yamada, et al.; Psychoneuroendocrinology 55, 81 (2015), Application(s): ELISA using mouse plasma, Abstract;
Social Isolation Disrupts Innate Immune Reponses in both Male and Female Prairie Voles and Enhances Agonistic Behavior in Female Prairie Voles (Microtus ochrogaster): M.A. Scotti, et al.; Horm. Behav. (2015), Application(s): ELISA, Abstract;
Somatostatin, neuronal vulnerability and behavioral emotionality: L.C. Lin, et al.; Mol. Psychiatry (2015), Application(s): ELISA, Abstract;
Sources of diel variation in energetic physiology in an Arctic-breeding, diving seaduck: R. J. Steenweg, et al.; Gen. Comp. Endocrinol. (2015), Application(s): ELISA using female eiders, Abstract;
Sex differences and similarities in the neuroendocrine regulation of social behavior in an African cichlid fish: L.A. O'Connell, et al.; Horm. Behav. 64, 468 (2013), Application(s): EIA using fish plasma, Abstract;
Comparative patterns of adrenal activity in captive and wild Canada lynx (Lynx canadensis) : K.V. Fanson, et al.; J. Comp. Physiol. B. 182, 157 (2012), Application(s): EIA using lynx feces, Abstract;
Environmental enrichment during rearing alters corticosterone levels, thymocyte numbers, and aggression in female BALB/c mice: E.K. Hutchinson, et al.; J. Am. Assoc. Lab. Anim. 51, 18 (2012), Application(s): ELISA using mouse urine, Abstract; Full Text
Oral gavage in rats: animal welfare evaluation: P.V. Turner, et al.; J. Am. Assoc. Lab. Anim. Sci. 51, 25 (2012), Application(s): EIA using rat feces, Abstract; Full Text
Periodic maternal separation decreases hippocampal neurogenesis without affecting basal corticosterone during the stress hyporesponsive period, but alters HPA axis and coping behavior in adulthood: N. Lajud, et al.; Psychoneuroendocrinology 37, 410 (2012), Application(s): Corticosterone levels in rat plasma, Abstract;
Role of the pituitary–adrenal axis in granulocyte-colony stimulating factor-induced neuroprotection against hypoxia–ischemia in neonatal rats: M.S. Charles, et al.; Neurobiol. Dis. 47, 29 (2012), Application(s): ELISA using rat plasma, Abstract;
The CRF1 receptor antagonist SSR125543 attenuates long-term cognitive deficit induced by acute inescapable stress in mice, independently from the hypothalamic pituitary adrenal axis: J. Philbert, et al.; Pharmacol. Biochem. Behav. 102, 415 (2012), Application(s): ELISA using mouse plasma, Abstract;
Effects of short-term fasting in male Sprague-Dawley rats: M.H. Nowland, et al.; Comp. Med. 61, 138 (2011), Application(s): Rat serum corticosterone, Abstract; Full Text
Pumilio-2 function in the mouse nervous system: H. Siemen, et al.; PLoS One 6, e25932 (2011), Application(s): Feces and serum corticosterone, Abstract; Full Text
Restraint stress and repeated corticotrophin-releasing factor receptor activation in the amygdala both increase amyloid-beta precursor protein and amyloid-beta peptide but have divergent effects on brain-derived neurotrophic factor and pre-synaptic proteins in the prefrontal cortex of rats: Ray, et al.; Neuroscience 184, 139 (2011),
Strain differences in the neurochemical response to chronic restraint stress in the rat: Relevance to depression: C.M. O'Mahony, et al.; Pharmacol. Biochem. Behav. 97, 690 (2011), Application(s): Rat plasma corticosterone, Abstract;
Stress and glucocorticoids regulated corticotropin releasing factor in rat prefrontal cortex: Q.Y. Meng, et al.; Mol. Cell. Endocrinol. 342, 54 (2011), Application(s): Corticosterone levels in rat plasma, Abstract;
Alcohol extract of Echinacea pallida reverses stress-delayed wound healing in mice: Z. Zhai, et al.; Phytomedicine 16, 669 (2009), Application(s): Mouse plasma corticosterone , Abstract; Full Text
Dietary sodium manipulation during critical periods in development sensitize adult offspring to amphetamines: F. Flynn, et al. ; Am. J. Physiol. Regul. Integr. Comp. Physiol. 295, R899 (2008), Application(s): EIA using rat plasma, Abstract;
Lack of adrenomedullin in the mouse brain results in behavioral changes, anxiety, and lower survival under stress conditions: A. Martinez, et al. ; PNAS 105, 12581 (2008), Application(s): EIA using mouse serum, Abstract;
MCP-1-deficient mice show reduced neuroinflammatory responses and increased peripheral inflammatory responses to peripheral endotoxin insult: L. Van, et al.; J. Neuroinflammation 5, U1 (2008), Application(s): EIA using mouse serum, Abstract; Full Text
The probiotic Bifidobacteria infantis: An assessment of potential antidepressant properties in the rat: L. Desbonnet, et al. ; J. Psychiatr. Res. 43, 164 (2008), Application(s): EIA using rat plasma, Abstract;
Streptozotocin induces G(2) arrest in skeletal muscle myoblasts and impairs muscle growth in vivo: T. Hawke, et al. ; Am. J. Physiol., Cell Physiol. 292, C1033 (2007), Application(s): EIA using rat serum, Abstract;
The anorexic effect of alpha-melanocyte-stimulating hormone ismediated by corticotrophin-releasing factor in chicks: T. Tachibana, et al. ; Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 147, 173 (2007), Application(s): EIA using avian plasma, Abstract;
Leptin Corrects Host Defense Defects after Acute Starvation in Murine Pneumococcal Pneumonia : P. Mancuso, et al.; Am. J. Respir. Crit. Care Med. 173, 212 (2006), Application(s): ELISA using mouse whole blood, Abstract; Full Text
CRH stimulation of corticosteroids production in melanocytes is mediated by ACTH: A. Slominski, et al. ; Am. J. Physiol. Endocrinol. Metab. 288, E701 (2005), Application(s): EIA using human culture supernatants, Abstract;
Early life immune challenge alters innate immune responses to lipopolysaccharide: implications for host defense as adults: S. Ellis, et al. ; FASEB J. 19, 1519 (2005), Application(s): EIA using rat plasma, Abstract;
Hypertension from targeted ablation of chromogranin A can be rescued by the human ortholog: N.R. Mahapatra, et al. ; J. Clin. Invest. 115, 1942 (2005), Application(s): EIA using mouse cell lysates, Abstract;
Stress hormones: a link between maternal condition and sex-biased reproductive investment: O.P. Love, et al.; Am. Nat. 166, 751 (2005), Application(s): EIA using avian plasma, Abstract;
Urinary corticosterone measures: effects of strain and social rank in BKW and CD-1 mice: A.E. Fitchett, et al.; Behav. Processes 70, 168 (2005), Application(s): ELISA using mouse urine, Abstract;

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Corticosterone ELISA kit 

Highly sensitive corticosterone ELISA kit, species independent, used for animal stress research.
ELISA, Colorimetric detection | Print as PDF
ADI-901-097 5x96 wells 1,290.00 USD

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