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DHEA ELISA kit

Highly sensitive ELISA, measuring DHEA in culture supernatants, plasma, serum, saliva, and urine from any species.
 
ADI-900-093 96 wells 342.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-901-093 (5x96 wells)
  • Specific with low reactivity to structurally related molecules
  • Highly reproducible with less than 9% inter- and intra-assay variation
  • Highly sensitive, detecting low levels of DHEA
  • Fast results for up to 37 samples in duplicate in 5 hours
The DHEA EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. This kit has low reactivity to structurally related molecules and offers sensitive measurement of DHEA in a variety of sample types.
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Product Specification

Alternative Name:Dehydroepiandrosterone
 
Sensitivity:2.9 pg/ml (range 12.21 - 50,000 pg/ml)
 
Assay Time:5 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of DHEA in culture supernatants, plasma, serum, saliva, and urine from any species.
 
Species reactivity:Species independent
 
Crossreactivity:DHEA (100%), DHEA sulfate (30.0%), Androstenedione (0.73%), Androsterone (0.29%), Pregnenolone (0.28%), Testosterone (0.10%), Progesterone (0.06%), Estradiol (0.04%) and <0.02%: 20-hydroxycholesterol, Androsterone sulphate, DHEA sodium sulphate, 17-hydroxyepipregnanolone, 19-hydroxyandrostendione, 17-hydroxyprogesterone, Cortisol
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Kit/Set Contains:GxR IgG Microtiter plate, Assay buffer, Conjugate, Antibody, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:Dehydroepiandrosterone (DHEA) is a 17-ketosteroid produced by side chain cleavage of 17-hydroxypregnenolone. DHEA is reversibly converted to its sulfate ester (DHEA-S) and Androstendiol. The human adrenal glands secrete both DHEA and DHEA-S which in turn serve as the precursors for both estrogenic and androgenic steroids. DHEA has been linked to a number of physiological conditions including depression, systemic lupus erythematosus, and rheumatoid arthritis.
 

Product Literature References

Increased Muscular 5α-Dihydrotestosterone in Response to Resistance Training Relates to Skeletal Muscle Mass and Glucose Metabolism in Type 2 Diabetic Rats: N. Horii, et al.; PLoS One 11, e0165689 (2016), Application(s): Plasma & skeletal muscle extracts, Abstract; Full Text
Oxysterol-related-binding-protein related Protein-2 (ORP2) regulates cortisol biosynthesis and cholesterol homeostasis: T. Escajadillo, et al.; Mol. Cell. Endrocrinol. 427, 73 (2016), Application(s): Measured DHEA levels, Abstract;
Responses of sex steroid hormones to different intensities of exercise in endurance athletes: K. Sato, et al.; Exp. Physiol. 101, 168 (2016), Application(s): DHEA quantification using human serum, Abstract;
Differences in leukocyte profile, gene expression, and metabolite status of dairy cows with or without sole ulcers: K. O'Driscoll, et al.; J. Dairy Sci. 98, 1685 (2015), Application(s): DHEA quantification using bovine plasma, Abstract;
Sphingosine-1-phosphate rapidly increases cortisol biosynthesis and the expression of genes involved in cholesterol uptake and transport in H295R adrenocortical cells: N.C. Lucki, et al.; Mol. Cell Endocrinol. 348, 165 (2012), Application(s): EIA using cell culture supernatants, Abstract; Full Text

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