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Higher throughput - measure up to 38 samples in duplicate in just 3 hours
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Easy-to-use - works with any standard colorimetric plate reader, no specialized training required
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Ready-to-use liquid color-coded reagents save time and reduce errors
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More sensitive than other commercially available kits
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Specific - low reactivity to structurally related molecules
The 11-dehydro-TXB2 EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. This kit offers ultrasensitive quantification of 11-d-TXB2 in a variety of sample matrices with negligible reactivity with related molecules in a convenient 96-well format offers higher throughput analysis compared to conventional analytical methods.
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Product Details
| Alternative Name: | 11-dehydro-Thromboxane B2 |
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| Sensitivity: | 4.31 pg/ml (range 9.8 - 10,000 pg/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of 11-dehydro-TXB2 in culture supernatants and urine from any species. Cited samples include serum. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Species independent
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| Use/Stability: | Store all components at +4°C, except standard and conjugate at -20°C. |
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| Shipping: | Blue Ice |
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| Contents: | GxR IgG Microtiter plate, Assay buffer, Conjugate, Antibody, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
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| Scientific Background: | Thromboxane A2 (TXA2) is involved in platelet aggregation, vasoconstriction and reproductive functions, but has a half life of only 37 seconds under physiological conditions. Thromboxane B2 (TXB2) is the stable product of the non-enzymatic hydration of TXA2, thus the production of TXA2 in vivo is typically monitored by measurement of TXB2 and its metabolite 2,3-dinor TXB2. 11-dehydro-TXB2 is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma produced by its dehydrogenation by the enzyme 11-OH-dehydrogenase. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol.
12, 774086 (2022),
Application(s): ELISA kit using hemolymph,
Abstract;
Full Text
Effect of aspirin treatment on abacavir-associated platelet hyperreactivity in HIV-infected patients: E. Falcinelli, et al.; Int. J. Cardiol.
263, 118 (2018),
Application(s): Human Urine,
Abstract;
Moderate intake of docosahexaenoic acid raises plasma and platelet vitamin E levels in cystic fibrosis patients: E. Vericel, et al.; Prostaglandins Leukot. Essent. Fatty Acids
115, 41 (2016),
Application(s): Basal formation of platelet thromboxane B2 and its urinary 11-dehydro metabolite,
Abstract;
Full Text
Moderate oral supplementation with docosahexaenoic acid improves
platelet function and oxidative stress in type 2 diabetic patients: E. Véricel, et al.; Thromb. Haemost.
114, 289 (2015),
Application(s): Platelet isolates from human blood samples,
Abstract;
Perioperative management of aspirin resistance after off‐pump coronary artery bypass grafting: possible role for aprotinin: R.S. Poston, et al.; Transfusion
48, 39S (2008),
Application(s): EIA using a variety of human serum,
Abstract;
Full Text
Simultaneous “hybrid” percutaneous coronary intervention and minimally invasive surgical bypass grafting: feasibility, safety, and clinical outcomes: B. Reicher, et al.; Am. Heart J.
155, 661 (2008),
Application(s): EIA using a variety of human serum,
Abstract;
Full Text
The effects of COX-2 selective and non-selective NSAIDs on the initiation and progression of atherosclerosis in ApoE-/- mice: J. Metzner, et al.; J. Mol. Med.
85, 623 (2007),
Application(s): EIA using a variety of human urine,
Abstract;
Full Text
Off-pump coronary artery bypass leads to a regional hypercoagulable state not detectable using systemic markers: Z.N. Kon, et al.; Innovations (Phila)
1, 232 (2006),
Application(s): EIA using a variety of human serum,
Abstract;
Full Text
Increased susceptibility to pulmonary hypertension in heterozygous BMPR2-mutant mice: Y. Song, et al.; Circulation
112, 553 (2005),
Application(s): EIA using a variety of human urine,
Abstract;
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