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Osteopontin (rodent), ELISA kit

 
ADI-900-090A 96 wells 504.00 USD
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  • Fast - obtain results from up to 40 samples in duplicate in just 3 hours
  • Easy-to-use - liquid color-coded reagents reduce errors
  • Accurate - parallelism data demonstrate that the recombinant standard accurately reflects natural OPN in biological matrices
The Osteopontin (rodent), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours.
900-090A Parallelism
900-090A std curve
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900-090A Parallelism 900-090A std curve

Product Specification

Alternative Name:OPN, SPP1, Secreted phosphoprotein 1, Bone sialoprotein 1
 
Sensitivity:3.03 ng/ml (range 3.13 - 100 ng/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of mouse and rat osteopontin in culture supernatants, plasma, and urine. Cited sample type includes cerebrospinal fluid.
 
Species reactivity:Mouse, Rat
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Kit/Set Contains:Microtiter plate, Standard, Antibody, Conjugate, Assay buffer 35, TMB Substrate, Stop solution 2, Wash buffer concentrate
 
Scientific Background:Osteopontin (OPN) is an acidic extracellular matrix cell adhesion protein that is relatively abundant not only in bone matrix, plasma, urine, and milk, but is also found in malignant and atherogenic tissues. Phosphorylation, glycosylation and calcium modifications allow intact and fragmented Osteopontin to direct a variety of diverse responses including tissue remodeling, inflammation and cell survival. Plasma Osteopontin has been shown to be a positive indicator of colon and lung cancers as well as metastatic carcinomas. The notable presence of Osteopontin in a variety of tumors is strongly correlated to pathological stage, suggesting its critical role in tumor invasiveness, progression and metastasis. In addition, Osteopontin inhibits inducible nitric oxide synthase activity, thereby protecting tumor cells from NO-mediated macrophage cytotoxic attack. Osteopontin is found in atherosclerotic plaques and may drive a number of diabetic vascular pathologies.
 
UniProt ID:P10923 (mouse), P08721 (rat)
 

Product Literature References

Hydrogen peroxide regulates osteopontin expression through activation of transcriptional and translational pathways: A.N. Lyle, et al.; J. Biol. Chem. 289, 275 (2014), Application(s): ELISA using culture supernatants, Abstract;
In vivo overexpression of tissue-nonspecific alkaline phosphatase increases skeletal mineralization and affects the phosphorylation status of osteopontin: S. Narisawa, et al.; J. Bone Miner. Res. 28, 1587 (2013), Application(s): ELISA using mouse plasma, Abstract;
Nasal administration of recombinant osteopontin attenuates early brain injury after subarachnoid hemorrhage: B.C. Topkoru, et al.; Stroke 44, 3189 (2013), Application(s): ELISA using rat cerebrospinal fluid, Abstract;

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