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TNF-α (rat), ELISA kit

 
ADI-900-086A 96 wells 536.00 USD
Do you need bulk/larger quantities?
 
  • Sensitive - detect <30 pg/ml rat TNFα
  • Fast - fully quantitative results in just 3 hours
  • Easy-to-use - liquid color-coded reagents reduce errors
  • Save time, money, and precious sample compared to Western blot analysis
The TNF-α (rat), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm.
900-086A std curve AB28
900-086A std curve DCM
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900-086A std curve AB28 900-086A std curve DCM

Product Specification

Alternative Name:Tumor necrosis factor-α, TNFSF 2
 
Sensitivity:12.0 pg/ml (range 31.3 - 2000 pg/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of TNF-α in rat culture supernatants.
 
Species reactivity:Rat
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 28, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2
 
Scientific Background:The TNF-α (rat), ELISA kit is a complete kit for the quantitative determination of rat TNF-α in culture supernatants. Please read the complete kit insert before performing this assay. Tumor necrosis factor-alpha (TNF-α), also known as cachectin, is a 17.5kDa, 157 amino acid member of the TNF superfamily of cytokines that is a potent lymphoid factor with effects on a wide range of target cells. Active TNF-α is produced as both soluble and membrane-anchored trimers by macrophages, NK cells, and T- and B-lymphocytes. TNF exerts proinflammatory signals via binding and inducing trimerization of TNF-receptor 1 (TNF-R1) expressed on most normal and transformed cells, or to TNF-receptor 2 (TNF-R2), expressed on endothelial and most immune cells. TNF signaling regulates hematopeiesis, differentiation, endothelial cell activation, apoptosis, lipid metabolism, tumor progression, and immune surveillance, and dysregulation of TNF or its receptors is implicated in numerous disease states including cancer, osteoporosis, autoimmune diseases, diabetes, and atherosclerosis.
 
UniProt ID:P16599
 

Product Literature References

Long-term feeding of red algae (Gelidium amansii) ameliorates glucose and lipid metabolism in a high fructose diet-impaired glucose tolerance rat model: H.C. Liu, et al.; J. Food Drug Anal. (2016), Application(s): Determination of tumor necrosis factor-α, rat plasma,
Therapeutic role of ursolic acid on ameliorating hepatic steatosis and improving metabolic disorders in high-fat diet-induced non-alcoholic fatty liver disease rats: S. Li, et al.; PLoS One 9, e86724 (2014), Application(s): ELISA using rat serum, Abstract; Full Text

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