Big Endothelin-1 (rat), ELISA kit

The Big Endothelin-1 (rat), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results overnight + 1 hour.

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Sensitivity:	0.72 pg/ml (range 0.78 - 100 pg/ml)

Kit/Set Contains:	Microtiter plate, Labeled antibody concentrate, Antibody diluent, Assay buffer, Wash buffer concentrate, Standard, TMB Substrate, Stop solution

Application:	For the quantitative determination of rat Big ET-1 in culture supernatants, plasma, and serum.

Long Term Storage:	+4°C

Miscellaneous/General:	Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ET_A and ET_B. Activation of ET_A increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ET_B receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.