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Cysteinyl leukotriene ELISA kit

Highly sensitive ELISA kit enabling detection of LTC4/D4/E4 in 4 hours.
 
ADI-900-070 96 wells 372.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-901-070 (5x96 wells)
  • Sensitive measurement of LTC4/D4/E4, detecting as little as 26.6 pg/ml
  • High throughput format with results in 4 hours for up to 38 samples in duplicate
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
The Cysteinyl Leukotriene EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 4 hours. Absorbance is read at 405 nm. Safe non-radioactive measurement of LTC4/D4/E4. Ready-to-use liquid color-coded reagents reduce errors.
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Product Specification

Sensitivity:26.6 pg/ml (range 78.1 - 2,500 pg/ml)
 
Assay Time:4 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of Cysteinyl Leukotrienes (LTC4/D4/E4) in culture supernatants and urine from any species.
 
Species reactivity:Species independent
 
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:GxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene B4 (LTB4) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC4, LTD4, and LTE4) are formed by addition of cysteine derivatives to LTA4. The conversion of LTA4 to LTC4 by the enzyme LTC4 synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions and increased capillary permeability.
 

Product Literature References

Identifying diagnostic endocrine markers and changes in endometrial gene expressions during pyometra in cats: E. Jursza-Piotrowska & M.J. Siemieniuch; Reprod. Biol. 16, 174 (2016), Application(s): Concentrations of leukotrienes C4 in plasma, Abstract;
Paediatric obstructive sleep apnoea syndrome (OSAS) is associated with tonsil colonisation by Streptococcus pyogenes: E. Viciani, et al.; Sci. Rep. 6, 20609 (2016), Application(s): ELISA using culture supernatants, Abstract; Full Text
The 5-lipoxygenase inhibitor RF-22c potently suppresses leukotriene biosynthesis in cellulo and blocks bronchoconstriction and inflammation in vivo: A.M. Schaible, et al.; Biochem. Pharmacol. 112, 60 (2016), Application(s): ELISA using culture supernatants, Abstract;
Vitex agnus-castus dry extract BNO 1095 (Agnucaston®) inhibits uterine hyper-contractions and inflammation in experimental models for primary dysmenorrhea: J. Rohrl, et al.; Int. J. Phytomed. Phytotherapy 2, 20 (2016), Application(s): Inhibition of purified 5-lipoxygenase (5-LO) and cellular leukotriene biosynthesis in isolated human monocytes,
Altered secretion of selected arachidonic acid metabolites during subclinical endometritis relative to estrous cycle stage and grade of fibrosis in mares: K. Gajos, et al.; Theriogenology 84, 457 (2015), Application(s): ELISA using mare sample, Abstract;
Cinnamaldehyde is the main mediator of cinnamon extract in mast cell inhibition: Y. Hagenlocher, et al.; Eur. J. Nutr. 54, 1297 (2015), Application(s): ELISA using culture supernatants, Abstract;
Indirubin-3'-monoxime exerts a dual mode of inhibition towards leukotriene-mediated vascular smooth muscle cell migration: T. Blazevic, et al.; Cardiovasc. Res. 101, 522 (2014), Application(s): ELISA using culture supernatants, Abstract; Full Text
The novel benzimidazole derivative BRP-7 inhibits leukotriene biosynthesis in vitro and in vivo by targeting 5-lipoxygenase-activating protein (FLAP): C. Pergola, et al.; Br. J. Pharmacol. 171, 3051 (2014), Application(s): ELISA using culture supernatants, Abstract; Full Text
Effects of the rhizomes of Atractylodes japonica and atractylenolide I on allergic response and experimental atopic dermatitis: H. Lim, et al.; Arch. Pharm. Res. 35, 2007 (2012), Application(s): EIA using rat RBL-1 cells, Abstract;

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