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PGF ELISA kit

 
ADI-900-069 96 wells 342.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-901-069 (5x96 wells)
  • Broad dynamic range suitable for many sample types
  • Stable liquid color-coded reagents reduce errors
  • Convenient storage conditions
  • Results from up to 36 samples in duplicate in less than 3 hours
  • Reproducible results day-after-day and lot-after-lot
The PGF EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Broad dynamic range is suitable for a wide variety of sample types. Ready-to-use liquid color-coded reagents are safe, non-radioactive, and reduce laboratory errors.
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Product Specification

Alternative Name:Prostaglandin F2α
 
Sensitivity:6.71 pg/ml (range 3.05 - 50,000 pg/ml)
 
Assay Time:<3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of PGF in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample types include cell lysate and whole blood.
 
Species reactivity:Species independent
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:Prostaglandin F (PGF) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF have also been associated with preeclampsia.
 

Product Literature References

Colon cancer-derived myofibroblasts increase endothelial cell migration by glucocorticoid-sensitive secretion of a pro-migratory factor: Z. Drebert, et al.; Vascul. Pharmacol. 89, 19 (2017), Application(s): Determine the concentrations of prostanoids in conditioned medium from myofibroblasts and HUVECs, and in HUVEC lysates, Abstract;
Circulating retinoic acid levels and the development of metabolic syndrome: Y. Liu, et al.; J. Clin. Endocrinol. Metab. 101, 1686 (2016), Application(s): Measured plasma 8-iso-Prostaglandin F2, Abstract; Full Text
Electrochemical immunosensor for the determination of 8-isoprostane aging biomarker using carbon nanohorns-modified disposable electrodes: E.E Sánchez-Tirado, et al.; J. Electroanal. Chem. (2016),
Regulation of cyclooxygenase-2 expression in rat oviductal epithelial cells: Evidence for involvement of GPR30/Src kinase-mediated EGFR signaling: P. Popli, et al.; J. Steroid Biochem. Mol. Biol. 154, 130 (2015), Application(s): ELISA using rat cell lysate, Abstract;
Female preference for males depends on reproductive physiology in the African cichlid fish Astatotilapia burtoni: M.R. Kidd, et al.; Gen. Comp. Endocrinol. 180, 56 (2013), Application(s): ELISA using aquarium water, Abstract;
Measuring multiple hormones from a single water sample using enzyme immunoassays: C. Kidd, et al.; Gen. Comp. Endocrinol. 165, 277 (2010), Application(s): EIA using fish plasma and water samples, Abstract;
Discovery of ecopladib, an indole inhibitor of cytosolic phospholipase A2 alpha: K.L. Lee, et al. ; J. Med. Chem. 50, 1380 (2007), Application(s): EIA using human whole blood, Abstract;
Cloning, Expression, and Functional Characterization of Human Cyclooxygenase-1 Splicing Variants: Evidence for Intron 1 Retention: N. Qin, et al. ; J. Pharmacol. Exp. Ther. 315, 1298 (2005), Application(s): EIA using human cell lysates, Abstract;
17{beta}-Estradiol decreases vascular tone in cerebral arteries by shifting COX-dependent vasoconstriction to vasodilation: J.A. Ospina, et al. ; Am. J. Physiol. Heart Circ. Physiol. 285, H241 (2003), Application(s): EIA using rat cell lysates, Abstract;
Skeletal Muscle PGF2{alpha}and PGE2 in Response to Eccentric Resistance Exercise: Influence of Ibuprofen and Acetaminophen: T.A. Trappe, et al. ; J. Clin. Endocrinol. Metab. 86, 5067 (2001), Application(s): EIA using human cell lysates, Abstract;
Tumor Necrosis Factor-alpha Induces Cyclooxygenase-2 Expression and Prostaglandin Release in Brain Microvessel Endothelial Cells: K.S. Mark, et al. ; J. Pharmacol. Exp. Ther. 297, 1051 (2001), Application(s): EIA using bovine cell lysates, Abstract;
Phase-dependent influence of nonsteroidogenic cells on steroidogenesis and prostaglandin production by the human corpus luteum: B.E. Friden, et al. ; Fertil. Steril. 73, 359 (2000), Application(s): EIA using human culture supernatants, Abstract;
Prostaglandin-Independent Anovulatory Mechanism of Indomethacin Action: Inhibition of Tumor Necrosis Factor {alpha}-Induced Sheep Ovarian Cell Apoptosis: W.J. Murdoch, et al. ; Biol. Reprod. 61, 1655 (1999), Application(s): EIA using sheep cell lysates, Abstract;

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Do you need bulk/larger quantities?
 

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