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cAMP ELISA kit

Most sensitive (0.039 pmol/ml) colorimetric kit for extracellular cAMP quantification.
 
ADI-900-067 96 wells 330.00 USD
 
Replaces Prod. #: BML-AK255

Alternative size available: ADI-901-067 (5x96 wells)
  • Trusted – regularly cited in peer-reviewed publications
  • Sensitive – optional acetylation protocol increases sensitivity 10-fold (0.039 pmol/ml)
  • Rapid – results in just 3 hours from up to 39 samples in duplicate
  • Easy-to-use – pre-coated plate and liquid color-coded reagents reduce errors
  • Simple – efficient and well-established sample handling protocols
  • Reliable – consistent lot-to-lot performance
This is a colorimetric competitive enzyme immunoassay kit for the quantitative determination of extracellular cAMP. Sensitivity is increased 10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The ready-to-use liquid color-coded reagents provided reduce error.
900-067-Kit-Graph
900-067 lot-to-lot
Lot-to-lot consistency -- Graph demonstrates the robust and reproducible nature of the competitive cAMP ELISA kit (Prod. No. ADI-900-067) showing standard curves from 10 lots manufactured over 6 years.
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900-067-Kit-Graph 900-067 lot-to-lot

Product Specification

Alternative Name:cyclic AMP
 
Sensitivity:(non-acetylated) 0.30 pmol/ml; (acetylated) 0.039 pmol/ml (range (non-acetylated) 0.78 - 200 pmol/ml;(acetylated) 0.078 - 20 pmol/ml)
 
Assay Time:3 hours
 
Application:For the quantitative determination of cAMP in culture supernatants, serum, and saliva from any species. Cited sample types include cell lysate (ref. 1), tissue (ref. 2), and urine (ref. 3).
 
Crossreactivity:cyclic AMP (100%), AMP (0.33%), ATP (0.12%) and <0.001%: cyclic GMP, GMP, GTP, cyclic UMP, CTP
 
Use/Stability:Store all components at +4°, except standard and conjugate at -20°.
 
Kit/Set Contains:GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer 2, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine
 
Miscellaneous/General:Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.
 

Product Literature References

Characterization of Thyrotropin Receptor Antibody-Induced Signaling Cascades: S.A. Morshed, et al. ; Endocrinology 150, 519 (2009), Application(s): EIA using hamster & rat cell lysates (ref. 1), Abstract;
Locomotor activity, ultrasonic vocalization and oxytocin levels in infant CD38 knockout mice: H. Higashida, et al. ; Neurosci. Lett. 448, 67 (2008), Application(s): EIA using mouse culture supernatants & tissue (ref. 2), Abstract;
The phosphodiesterase 5 inhibitor sildenafil stimulates angiogenesis through a protein kinase G/MAPK pathway: A. Papapetropoulos, et al. ; J. Cell. Physiol. 211, 197 (2007), Application(s): EIA using human cell lysates, Abstract;
The phosphodiesterase 5 inhibitor sildenafil stimulates angiogenesis through a protein kinase G/MAPK pathway: K.K. Parker, et al. ; Neurochem. Int. 50, 109 (2007), Application(s): EIA using hamster cell lysates, Abstract;
High-salt diet impairs vascular relaxation mechanisms in rat middle cerebral arteries: J.H. Lombard, et al. ; Am. J. Physiol. Heart Circ. Physiol. 284, H1124 (2003), Application(s): EIA using rat cell lysates, Abstract;
Phosphoinositide 3-Kinase Activity Is Required for Biphasic Stimulation of Cyclic Adenosine 3',5'-Monophosphate by Relaxin: B.T. Nguyen, et al. ; Mol. Endocrinol. 17, 1075 (2003), Application(s): EIA using human culture supernatants & cell lysates, Abstract;
D2-like receptor-mediated inhibition of Na+-K+-ATPase activity is dependent on the opening of K+ channels: P. Gomes, et al. ; Ren. Physiol. 283, F114 (2002), Application(s): EIA using opossum cell lysates, Abstract;
Renal responses to plasma volume expansion and hyperosmolality in fasting seal pups: R.M. Ortiz, et al. ; Am. J. Physiol. Regul. Integr. Comp. Physiol. 282, R805 (2002), Application(s): EIA using seal urine (ref. 3), Abstract;
Urocortin, But Not Urocortin II, Protects Cultured Hippocampal Neurons from Oxidative and Excitotoxic Cell Death via Corticotropin-Releasing Hormone Receptor Type I: W.A. Pedersen, et al. ; J. Neurosci. 22, 404 (2002), Application(s): EIA using rat cell lysates, Abstract;
The Involvement of Tyrosine Kinases, Cyclic AMP/Protein Kinase A, and p38 Mitogen-Activated Protein Kinase in IL-13-Mediated Arginase I Induction in Macrophages: Its Implications in IL-13-Inhibited Nitric Oxide Production: C.I. Chang, et al. ; J. Immunol. 165, 2134 (2000), Application(s): EIA using mouse cell lysates, Abstract;

General Literature References

Central role for cAMP signaling in pigmentation and UV resistance: J. D'Orazio & D.E. Fisher; Cell Cycle 10, 8 (2011), Abstract;

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