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Direct cAMP ELISA kit

Most sensitive (0.037 pmol/ml) colorimetric kit for intracellular cAMP quantification.
 
ADI-900-066 96 wells 378.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: BML-AK205

Alternative size available: ADI-901-066 (5x96 wells)
  • Highly sensitive with an optional acetylation protocol which increases sensitivity 10-fold (0.037 pmol/ml)
  • High throughput format, results for up to 39 samples in duplicate in just 3 hours
  • Flexible assay, species independent
  • Easy-to-use pre-coated plate and liquid color-coded reagents reduce errors
  • Efficient and well-established sample handling protocols
  • Widely cited in peer-reviewed publications
This is a colorimetric competitive immunoassay kit for the quantitative determination of intracellular cAMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased 10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The large signal-to-background ratio offers superior sensitivity compared to competitors. This kit lets you easily assess adenylyl cyclase activation by GPCRs.
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900-066 non-ace std curve
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Product Specification

Alternative Name:cyclic AMP
 
Sensitivity:(non-acetylated) 0.39 pmol/ml; (acetylated) 0.037 pmol/ml (range (non-acetylated) 0.78 - 200 pmol/ml;(acetylated) 0.078 - 20 pmol/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of cAMP in cell lysates and tissue from any species. Cited sample types include tissue culture.
 
Species reactivity:Species independent
 
Crossreactivity:cyclic AMP (100%), AMP (0.33%), ATP (0.12%) and <0.001%: cyclic GMP, GMP, GTP, cyclic UMP, CTP
 
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:GxR IgG Microtiter plate, Conjugate, Antibody, 0.1M HCl, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine
 
Scientific Background:Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.
 

Product Literature References

Aldosterone does not alter endothelin B receptor signaling in the inner medullary collecting duct: N. Ramkumar, et al.; Physio Rep. 5, e13167 (2017), Application(s): Measurement of cAMP in mouse inner medullary collecting duct cell line., Abstract; Full Text
Investigation of triamterene as an inhibitor of the TGR5 receptor: identification in cells and animals: Y. Li, et al.; Drug Des. Devel. Ther. 11, 1127 (2017), Application(s): Measurement of intracellular cAMP levels in rat NCI-H716 cells., Abstract; Full Text
A Putative Association of a Single Nucleotide Polymorphism in GPR126 with Aggressive Periodontitis in a Japanese Population: J. Kitagaki, et al.; PLoS One 11, e0160765 (2016), Application(s): Intracellular cAMP measurement, HEK293T and HPDL cells, Abstract; Full Text
Acute upregulation of neuronal mitochondrial type-1 cannabinoid receptor and it’s role in metabolic defects and neuronal apoptosis after TBI: Z. Xu, et al.; Mol. Brain 9, 75 (2016), Application(s): Cyclic AMP assay, isolated mitochondria, Abstract; Full Text
Cortisol-induced immune suppression by a blockade of lymphocyte egress in traumatic brain injury: T. Dong, et al.; J. Neuroinflammation 13, 197 (2016), Application(s): Measurement of intracellular cAMP, mouse T cells, Abstract; Full Text
Cyclic AMP effectors regulate myometrial oxytocin receptor expression: A. Yulia, et al.; Endocrinology 157, 4411 (2016), Application(s): Measurement of intracellular cAMP in myometrial tissue, Abstract; Full Text
LH and FSH promote migration and invasion properties of a breast cancer cell line through regulatory actions on the actin cytoskeleton: A.M. Sanchez, et al.; Mol. Cell. Endocrinol. 437, 22 (2016), Application(s): Measurement of cAMP in T-47D breast cancer cells, Abstract;
Neurotrophic and neuroprotective effects of oxyntomodulin in neuronal cells and a rat model of stroke: Y. Li, et al.; Exp Neurol. 14, 104 (2016), Application(s): Measurement of intracellular cAMP, Supernatants, Abstract;
New insight into the role of metabolic reprogramming in melanoma cells harboring BRAF mutations: A. Ferretta, et al.; Biochem. Biophys. Acta 1863, 2710 (2016), Application(s): Cyclic adenosine monophosphate (cAMP) assay in cell lysates, Abstract;
Phosphodiesterase Inhibition as a Mechanism of Ion Channel Modulation by Celecoxib: C.J. Robakowski; (2016), (Master's thesis); Application(s): Quantitative determination of cyclic AMP in cells treated with hydrochloric acid, Full Text
Protective Effect of a GLP-1 Analog on Ischemia-Reperfusion Induced Blood-Retinal Barrier Breakdown and Inflammation: A. Goncalves, et al.; Invest. Ophthalmol. Vis. Sci. 57, 2584 (2016), Application(s): Intracellular levels of cAMP were measured, Abstract; Full Text
Protein Kinase A subunit balance regulates lipid metabolism in Caenorhabditis elegans and mammalian adipocytes: J.H. Lee, et al.; J. Biol. Chem. 291, 20315 (2016), Application(s): cAMP concentration measurement in Cenorhabditis elegans and mammalian cell lysates, Abstract; Full Text
Role of protein kinase A and class II phosphatidylinositol 3-kinase C2β in the downregulation of KCa3.1 channel synthesis and membrane surface expression by lyso-globotriaosylceramide: J.Y. Choi & S. Park; Biochem. Biophys. Res. Commun. 470, 907 (2016), Application(s): Measured cAMP levels, Abstract;
The Angiotensin-(1-7)/Mas axis improves pancreatic β-cell function in vitro and in vivo: A. Sahr, et al.; Endocrinology 157, 4677 (2016), Application(s): Determination of cyclic AMP (cAMP) concentrations, mouse lysates, Abstract;
Vibratory stimulation enhances thyroid epithelial cell function: A.P. Wagner, et al.; Biochem. Biophys. Rep. 8, 376 (2016), Application(s): FRTL-5 cells,
Disruption of NHE8 Expression Impairs Leydig cell function in the testes: H. Xu, et al.; Am. J. Physiol. Cell Physiol. 308, C330 (2015), Application(s): Measurement of cAMP concentration in cell lysates, Abstract; Full Text
Impaired enzymatic defensive activity, mitochondrial dysfunction and proteasome activation are involved in RTT cells oxidative damage: C. Cervellati, et al.; Biochim. Biophys. Acta. 1852, 2066 (2015), Application(s): ELISA using human supernatant, Abstract;
Investigating The Glucagon Receptor and GLP-1 Receptor Activity of Oxyntomodulin-Like Analogues in Male Wistar Rats: S.L. Price, et al. ; Curr. Ther. Res. 77, 111 (2015), Application(s): ELISA using cell lysates,
Limonin, a Component of Dictamni Radicis Cortex, Inhibits Eugenol-Induced Calcium and cAMP Levels and PKA/CREB Signaling Pathway in Non-Neuronal 3T3-L1 Cells: Y.C. Yoon, et al.; Molecules 20, 22128 (2015), Application(s): cAMP measurement in 3T3-L1 cells, Abstract; Full Text
MEK Inhibitors Reverse cAMP-Mediated Anxiety in Zebrafish: P.R. Lundegaard, et al.; Chem. Biol. 15, 330 (2015), Application(s): ELISA determining cAMP concentration in zebrafish larvae, Abstract;
Stable G protein-effector complexes in striatal neurons: mechanism of assembly and role in neurotransmitter signaling: K. Xie, et al.; eLife 4, e10451 (2015), Application(s): cAMP measurement of cell supernatants, Abstract; Full Text
Temporal cAMP signaling selectivity by natural and synthetic MC4R agonists: B.M. Molden, et al.; Mol. Endocrinol. 29, 1619 (2015), Application(s): cAMP determination by immunoassay, Abstract; Full Text
The aglycone of ginsenoside Rg3 enables glucagon-like peptide-1 secretion in enteroendocrine cells and alleviates hyperglycemia in type 2 diabetic mice: K.S. Kim, et al. ; Sci. Rep. 5, 18325 (2015), Application(s): Measurement of intracellular cAMP, Abstract; Full Text
The cAMP phosphodiesterase Prune localizes to the mitochondrial matrix and promotes mtDNA replication by stabilizing TFAM: F. Zhang, et al.; EMBO Rep. 16, 520 (2015), Application(s): ELISA, Abstract; Full Text
The neuroprotective effect of lithium in cannabinoid dependence is mediated through modulation of cyclic AMP, ERK1/2 and GSK-3β phosphorylation in cerebellar granular neurons of rat: H.R. Rahimi, et al.; Iran. J. Pharm. Res. 14, 1123 (2015), Application(s): ELISA using rat supernatant, Abstract; Full Text
Ubiquitin is a versatile scaffold protein for the generation of molecules with de novo binding and advantageous drug-like properties: F. Job, et al.; FEBS Open Bio 5, 579 (2015), Application(s): ELISA using cell lysates, Abstract; Full Text
Adenosine 2A receptor promotes collagen production by human fibroblasts via pathways involving cyclic AMP and AKT but independent of Smad2/3: M. Perez-Aso, et al.; FASEB J. 28, 802 (2014), Application(s): Intracellular cAMP was measured, Abstract; Full Text
Cyclin-dependent kinase 5 in the ventral tegmental area regulates depression-related behaviors: P. Zhong, et al.; J. Neurosci. 34, 6352 (2014), Application(s): EIA using mouse ventral tegmental area (VTA) homogenates, Abstract; Full Text
DCC is expressed in a CD166-positive subpopulation of chondrocytes in human osteoarthritic cartilage and modulates CRE activity: A.K. Bosserhoff, et al.; Int. J. Clin. Exp. Pathol. 7, 1947 (2014), Application(s): EIA using chondrocyte homogenates, Abstract; Full Text
Delocalization of Endogenous A-kinase Antagonizes Rap1-Rho-α2C-Adrenoceptor Signaling in Human Microvascular Smooth Muscle Cells: H.K. Motawea, et al.; J. Cytol. Mol. Biol. 1, 9 (2014), Application(s): Measurement of intracellular cAMP in human microVSM transduced with Ht31-P or Ht31, Abstract; Full Text
Dopamine-2 receptor activation suppresses PACAP expression in gonadotrophs: S.J. Winters, et al.; Endocrinology 155, 2647 (2014), Application(s): Cell lysates were assayed, non-acetylated, Abstract; Full Text
Global analysis of the HrpL regulon in the plant pathogen Pseudomonas syringae pv. tomato DC3000 reveals new regulon members with diverse functions: H.N. Lam, et al.; PLoS One 9, e106115 (2014), Application(s): EIA using leaf extracts from N. benthamiana, Abstract; Full Text
High throughput synthetic lethality screen reveals a tumorigenic role of adenylate cyclase in fumarate hydratase-deficient cancer cells: M. Boettcher, et al.; BMC Genomics 15, 158 (2014), Application(s): EIA using human hereditary leiomyomatosis and renal cell carcinoma (HLRCC) lysates, Abstract; Full Text
Identification of plumericin as a potent new inhibitor of the NF-κB pathway with anti-inflammatory activity in vitro and in vivo: N. Fakhrudin, et al.; Br. J. Pharmacol. 171, 1676 (2014), Application(s): EIA using HUVECtert lysates (acetylated samples), Abstract; Full Text
Lipid droplet protein LID-1 mediates ATGL-1-dependent lipolysis during fasting in Caenorhabditis elegans: J.H. Lee, et al.; Mol. Cell. Biol. 34, 4165 (2014), Application(s): cAMP concentrations were measured, Abstract; Full Text
Low concentrations of metformin suppress glucose production in hepatocytes through AMP-activated protein kinase (AMPK): J. Cao, et al.; J. Biol. Chem. 289, 20435 (2014), Application(s): cAMP levels in Cellular lysates were determined, Abstract; Full Text
Prostaglandin E2 promotes features of replicative senescence in chronically activated human CD8+ T cells: J.P. Chou, et al.; PLoS One 9, e99432 (2014), Application(s): EIA using cell culture supernatants, Abstract; Full Text
Regulator of G-protein signaling 6 (RGS6) promotes anxiety and depression by attenuating serotonin-mediated activation of the 5-HT(1A) receptor-adenylyl cyclase axis: A. Stewart, et al.; FASEB J. 28, 1735 (2014), Application(s): cAMP Measurements were performed, Abstract; Full Text
Resveratrol and curcumin enhance pancreatic β-cell function by inhibiting phosphodiesterase activity: M. Rouse, et al.; J. Endocrinol. 223, 107 (2014), Application(s): EIA using mouse β-Min6 lysates and primary human islet lysates, Abstract; Full Text
The metabolite 5'-methylthioadenosine signals through the adenosine receptor A2B in melanoma: K. Limm, et al.; Eur J. Cancer 50, 2714 (2014), Application(s): Quantification of intracellular cAMP, Abstract;
Three intragenic suppressors of a GTPase-deficient allele of GNAS associated with McCune-Albright syndrome: K. Turcic, et al.; J. Mol. Endocrinol. 52, 321 (2014), Application(s): Assaying levels of cAMP in lysates, Abstract;
Age-dependent alterations in cAMP signaling contribute to synaptic plasticity deficits following traumatic brain injury: D.J. Titus, et al.; Neuroscience 231, 182 (2013), Application(s): EIA using rat cortex, hippocampus and thalamus homogenates, Abstract; Full Text
Calcium supplementation increases circulating cholesterol by reducing its catabolism via GPER and TRPC1-dependent pathway in estrogen deficient women: S. Li, et al.; Int. J. Cardiol. 168, 2548 (2013), Application(s): ELISA using rat liver tissue, Abstract;
Does 4-tert-octylphenol affect estrogen signaling pathways in bank vole Leydig cells and tumor mouse Leydig cells in vitro?: M. Kotula-Balak, et al.; Reprod. Toxicol. 39, 6 (2013), Application(s): cAMP was measured and quantitated, Abstract;
Liraglutide protects against amyloid-β protein-induced impairment of spatial learning and memory in rats: W.N. Han, et al.; Neurobiol Aging 34, 576 (2013), Application(s): EIA using rat brain homogenates, Abstract;
Molecular mechanisms underlying the rapid arrhythmogenic action of bisphenol A in female rat hearts: X. Gao, et al.; Endocrinology 154, 4607 (2013), Application(s): EIA using rat myocyte homogenates, Abstract; Full Text
Resveratrol prevents β-cell dedifferentiation in nonhuman primates given a high-fat/high-sugar diet: J.L. Fiori, et al.; Diabetes 62, 3500 (2013), Application(s): EIA using CHO cell supernatant, Abstract; Full Text
Role of AKAP79/150 protein in b1-adrenergic receptor trafficking and signaling in mammalian cells: X. Li, et al.; J. Biol. Chem. 288, 33797 (2013), Application(s): ELISA using murine cardiomyocytes, Abstract;
Rolofylline, an adenosine A1 receptor antagonist, inhibits osteoclast differentiation as an inverse agonist: W. He, et al.; Br. J. Pharmacol. 170, 1167 (2013), Application(s): EIA using bone marrow macrophage lysates (acetylated samples), Abstract; Full Text
Constitutive cholesterol-dependent endocytosis of melanocortin-4 receptor (MC4R) Is essential to maintain receptor responsiveness to α-melanocyte-stimulating hormone (α-MSH): F.K. McDaniel, et al.; J. Biol. Chem. 287, 21873 (2012), Application(s): ELISA using human neuronal cells, Abstract;
Drugs developed to treat diabetes, liraglutide and lixisenatide, cross the blood brain barrier and enhance neurogenesis: K. Junter & C. Hölscher ; BMC Neurosci. 13, 33 (2012), Application(s): ELISA analysis of mouse brain tissue, Abstract; Full Text
Gonadotropin-inhibitory hormone inhibits GnRH-induced gonadotropin subunit gene transcriptions by inhibiting AC/cAMP/PKA-dependent ERK pathway in LβT2 cells: Y.L. Son, et al.; Endocrinology 153, 2332 (2012), Application(s): Measurement of cAMP accumulation in cells, Abstract; Full Text
Liraglutide, a long-acting GLP-1 mimetic, and its metabolite attenuate inflammation after intracerebral hemorrhage: J. Hou, et al.; J. Cereb. Blood Flow Metab. 32, 2201 (2012), Application(s): ELISA analysis of mouse brain tissue, Abstract; Full Text
Pharmacological validation of Trypanosoma brucei phosphodiesterases as novel drug targets: H.P. de Koning, et al.; J. Infect. Dis. 206, 229 (2012), Application(s): EIA using Trypanosoma lysates, Abstract; Full Text
Regulation of pancreatic cancer by neuropsychological stress responses: a novel target for intervention: H.M. Schuller, et al.; Carcinogenesis 33, 191 (2012), Application(s): ELISA using human pancreatic cancer xenografts, Abstract;
Acylated and unacylated ghrelin attenuate isoproterenol-induced lipolysis in isolated rat visceral adipocytes through activation of phosphoinositide 3-kinase γ and phosphodiesterase 3B: A. Baragli, et al.; Biochim. Biophys. Acta 1811, 386 (2011), Application(s): EIA using rat adipocyte extracts, Abstract;
Characterization of the differential response of endothelial cells exposed to normal and elevated laminar shear stress: S.J. White, et al.; J. Cell. Physiol. 226, 2841 (2011), Application(s): ELISA using HUVEC, Abstract; Full Text
Rescue of expression and signaling of human luteinizing hormone G protein-coupled receptor mutants with an allosterically binding small-molecule agonist: C.L. Newton, et al.; PNAS 108, 7172 (2011), Application(s): Measured level of cAMP in cell supernatants, Abstract; Full Text
cAMP pulsing of denuded mouse oocytes increases meiotic resumption via activation of AMP-activated protein kinase: J. Chen, et al.; Reproduction 138, 759 (2009), Application(s): Concentrations were measured, Abstract; Full Text
Heat-Labile Enterotoxin Promotes Escherichia coli Adherence to Intestinal Epithelial Cells: P. Hardwidge, et al. ; J. Bacteriol. 191, 178 (2009), Application(s): EIA using porcine cell lysates, Abstract;
PPARγ agonists inhibit vasopressin-mediated anion transport in the MDCK-C7 cell line: C. Nofziger, et al.; Am. J. Physiol. Renal Physiol. 297, F55 (2009), Application(s): ELISA using MDCK-C7 cell lysates, Abstract; Full Text
Androgens transduce the G-alpha s-mediated activation of protein kinase A in prostate cells: Y. Daaka, et al. ; Cancer Res. 68, 3225 (2008), Application(s): EIA using human cell lysates, Abstract;
beta-Arrestin-1 mediates glucagon-like peptide-1 signaling to insulin secretion in cultured pancreatic beta cells: J. Olefsky, et al. ; PNAS 105, 6614 (2008), Application(s): EIA using rat cell lysates, Abstract;
Cyclic Adenosine Monophosphate Regulation of Ion Transport in Porcine Vocal Fold Mucosae: M. Sivasankar, et al.; Laryngoscope 118, 1511 (2008), Application(s): Measured cAMP concentrations in porcine samples, Abstract; Full Text
FMRP acts as a key messenger for dopamine modulation in the forebrain: M. Zhao, et al. ; Neuron 59, 634 (2008), Application(s): EIA using mouse cell lysates, Abstract;
Functional evidence for presence of lipid rafts in erythrocyte membranes: Gs-alpha in rafts is essential for signal transduction: Y. Takakuwa, et al. ; Am. J. Hematol. 83, 371 (2008), Application(s): EIA using human cell lysates, Abstract;
Regulation of G proteins by human 5-HT1a receptor TM3/i2 and TM5/i3 loop peptides: H.V. Thiagaraj, et al.; Neurochem. Int. 50, 109 (2007), Application(s): EIA using human cell lysates, Abstract;
Co-occurrence of Two Partially Inactivating Polymorphisms of MC3R Is Associated With Pediatric-Onset Obesity: N. Feng, et al. ; Diabetes 54, 2663 (2005), Application(s): EIA using human cell lysates, Abstract;
The hrpK Operon of Pseudomonas syringae pv. tomato DC3000 Encodes Two Proteins Secreted by the Type III (Hrp) Protein Secretion System: HopB1 and HrpK, a Putative Type III Translocator: T. Petnicki-Ocwieja, et al. ; J. Bacteriol. 187, 649 (2005), Application(s): EIA using plant cell lysates, Abstract;
High-salt diet impairs vascular relaxation mechanisms in rat middle cerebral arteries: J.H. Lombard, et al. ; Am. J. Physiol. Heart Circ. Physiol. 284, H1124 (2003), Application(s): EIA using rat cell lysates, Abstract;
Lysine 270 in the Third Intracellular Domain of the Oxytocin Receptor is an Important Determinant for G{alpha}q Coupling Specificity: M. Yang, et al. ; Mol. Endocrinol. 16, 814 (2002), Application(s): EIA using monkey cell lysates, Abstract;

General Literature References

Central role for cAMP signaling in pigmentation and UV resistance: J. D'Orazio & D.E. Fisher; Cell Cycle 10, 8 (2011), Abstract;

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