Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Complement C4a des Arg (human), ELISA kit

 
ADI-900-059 96 wells 483.00 USD
Do you need bulk/larger quantities?
 
  • Safe - non-radioactive measurement of C4a des Arg
  • Ready-to-use - convenient sample preparation reagents are provided
  • Higher throughput - results from up to 39 samples in duplicate in just 3 hours
The Complement C4a des Arg (human), EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. The kit comes with ready-to-use liquid color-coded reagents that reduce error in the lab and the necessesary sample preparation buffers.
900-059-Kit-Graph
Please mouse over
900-059-Kit-Graph

Product Specification

Sensitivity:0.76 ng/ml (range 0.78 - 200 ng/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of Complement C4a des arg in human EDTA plasma.
 
Species reactivity:Human
 
Crossreactivity:C4a des Arg (100%), C4 (2.14%), C5a des Arg (0.28%), C3 (0.23%), C3a des Arg (0.04%), C5 (0.02%)
 
Use/Stability:Store all components +4°, except conjugate -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer 10 concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Complement reagent A, Complement reagent B
 
Scientific Background:Activation of the complement cascade is initiated by multiple pathways, including C1q binding of antigen-antibody complexes on pathogens (classical pathway), binding of serum lectin to pathogen-unique mannose residue patterns (MBL pathway), or the spontaneous hydrolysis of C3 and subsequent processing to C3 convertase (alternative pathway). C3a and C4a are two complement cleavage products of 77- and 76-amino acid residues, respectively, that are liberated into the bloodstream and processed to their more stable metabolites C3a des Arg and C4a des Arg. Although both fragments have anaphylactic properties, C3a is considered a more potent stimulator of smooth muscle cell contraction, histamine release, and inflammation than C4a. Complement activation is associated with sepsis, respiratory distress syndrome, multi-organ failure, and transplant organ rejection.
 
UniProt ID:P0C0L4
 

Product Literature References

Systemic hypoxia enhances exercise-mediated bactericidal and subsequent apoptotic responses in human neutrophils: J.S. Wang, et al.; J. Appl. Physiol. 107, 1213 (2009), Application(s): ELISA using human plasma, Abstract;
Studies of the mechanisms of bradykinin generation in hereditary angioedema plasma: K. Joseph, et al. ; Ann. Allergy Asthma Immunol. 101, 279 (2008), Application(s): EIA using human plasma, Abstract;
Identification of plasma protein biomarkers associated with idiopathic pulmonary arterial hypertension: V.B. Abdul-Salam, et al.; Proteomics 6, 2286 (2006), Application(s): EIA using human plasma samples, Abstract;

Product Toolbox

PRODUCT RESOURCES

Datasheet
Assay Layout Sheet
MSDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By target:
Complement
By biological activity:
Complement Immunoassay kit
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Catalogs
Immunoassay Kits
Immunoassay Kits
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,