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Complement C3a des Arg (human), ELISA kit

 
ADI-900-058 96 wells 536.00 USD
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  • Ready-to-use - sample preparation reagents provided
  • Specific - low cross-reactivity to structurally related molecules
  • Sensitive - detect as little as 120 pg/ml
  • Higher throughput - results from up to 37 samples in duplicate in just 3 hours
The Complement C3a des Arg (human), EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. This kit comes ready-to-use with sample preparation reagents included. The Complement C3a des Arg (human), EIA kit is specific, offering low cross-reactivity to structurally related molecules, and sensitive with quantification of as little as 120 pg/mL Complement C3a des Arg.
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Product Specification

Sensitivity:0.120 ng/ml (range 0.313 - 20 ng/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of Complement C3a des arg in human EDTA plasma.
 
Species reactivity:Human
 
Crossreactivity:C3a des Arg (100%), C3 (1.28%), C4a des Arg (0.40%), C4 (0.31%), C5a des Arg (0.10%), C5 (0.05%)
 
Use/Stability:Store all components at +4°, except standard and conjugate at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer 10 concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Complement reagent A, Complement reagent B
 
Scientific Background:Activation of the complement cascade is initiated by multiple pathways, including C1q binding of antigen-antibody complexes on pathogens (classical pathway), binding of serum lectin to pathogen-unique mannose residue patterns (MBL pathway), or the spontaneous hydrolysis of C3 and subsequent processing to C3 convertase (alternative pathway). C3a and C4a are two complement cleavage products of 77- and 76-amino acid residues, respectively, that are liberated into the bloodstream and processed to their more stable metabolites C3a des Arg and C4a des Arg. Although both fragments have anaphylactic properties, C3a is considered a more potent stimulator of smooth muscle cell contraction, histamine release, and inflammation than C4a. Complement activation is associated with sepsis, respiratory distress syndrome, multi-organ failure, and transplant organ rejection.
 
UniProt ID:P01024
 

Product Literature References

Systemic hypoxia enhances exercise-mediated bactericidal and subsequent apoptotic responses in human neutrophils: J.S. Wang, et al.; J. Appl. Physiol. 107, 1213 (2009), Application(s): ELISA using human plasma, Abstract;

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