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TNF-α (mouse), ELISA kit

 
ADI-900-047 96 wells 467.00 USD
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  • Stable color coded reagents and pre-coated plates reduce errors
  • Assay up to 38 samples in just 5 hours
  • Save time, money, and precious sample compared to Western blot analysis
The TNF-α (mouse), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 5 hours. Absorbance is read at 450 nm.
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Product Specification

Alternative Name:Tumor necrosis factor-α, TNFSF 2
 
Sensitivity:3.9 pg/ml (range 31.25 - 2,000 pg/ml)
 
Assay Time:5 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of mouse TNF-α in culture supernatants and serum. Cited sample type includes tissue (ref. 1) and plasma.
 
Species reactivity:Mouse
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 16, Wash buffer concentrate, Standard, TMB Substrate, Stop solution
 
Scientific Background:Tumor Necrosis Factor-α (TNF-α) is a 17.5 kDa, 157 amino acid protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis. It is the primary mediator of immune regulation. The biosynthesis of TNF-α is tightly controlled, being produced in extremely small quantities in quiescent cells, but is a major secreted factor in activated cells.
 
UniProt ID:P06804
 

Product Literature References

Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages: Y.A. Bae, et al.; Korean J. Physiol. Pharmacol. 20, 415 (2016), Application(s): ELISA using culture supernatants, Abstract; Full Text
Antagonistic effects of Spirulina platensis against sub-acute deltamethrin toxicity in mice: Biochemical and histopathological studies: M. Abdel-Daim, et al. ; Biomed. Pharmacother. 77, 79 (2016), Application(s): Measurement of TNF-α in mouse serum, Abstract;
CLOCK modulates survival and acute lung injury in mice with polymicrobial sepsis: C.Y. Wang, et al.; Biochem. Biophys. Res. Commun. 478, 935 (2016), Application(s): Measurement of cytokine levels in serum and cell culture medium, Abstract;
Immunostimulating activity of polyhydric alcohol isolated from Taxus cuspidata: C.G. Lee, et al.; Int. J. Biol. Macromol. 85, 505 (2016), Application(s): Measured concentration of TNF-α released from the cells treated with T. cuspidata, Abstract;
Isolation and Identification of a Flavone Apigenin from Marine Red Alga Acanthophora spicifera with Antinociceptive and Anti-Inflammatory Activities: G.A. El Shoubaky, et al.; J. Exp. Neurosci. 10, 21 (2016), Application(s): IL-6 measured in mouse blood samples, Abstract; Full Text
The inhibiting effect of the Coptis chinensis polysaccharide on the type II diabetic mice: L. Cui, et al.; Biomed. Pharmacother. 81, 111 (2016), Application(s): ELISA using mouse blood samples, Abstract;
Ginseng marc-derived low-molecular weight oligosaccharide inhibits the growth of skin melanoma cells via activation of RAW264.7 cells: J.Y. Seo, et al.; Int. Immunopharmacol. 29, 344 (2015), Application(s): ELISA using culture supernatants, Abstract;
The immunostimulating activity of quercetin 3-O-xyloside in murine macrophages via activation of the ASK1/MAPK/NF-κB signaling pathway: J. Lee, et al.; Int. Immunopharmacol. 31, 88 (2015), Application(s): ELISA using cell culture supernatants, Abstract;
Antioxidative, anti-inflammatory, and matrix metalloproteinase inhibitory activities of 20 (S)-ginsenoside Rg3 in cultured mammalian cell lines: Y.M. Shin, et al.; Mol. Biol. Rep. 40, 269 (2013), Application(s): EIA using culture supernatants from RAW264.7 macrophage cells, Abstract;
Early systemic inflammatory response in mice after a single oral gavage with live Escherichia coli is evidenced by increased TNF-alpha and nitric oxide production: A. Nemec, et al.; Res. Vet. Sci. 92, 401 (2012), Application(s): EIA using mouse plasma, Abstract;
Cilostazol enhances apoptosis of synovial cells from rheumatoid arthritis patients with inhibition of cytokine formation via Nrf2-linked heme oxygenase 1 induction: S.Y. Park, et al.; Arthritis Rheum. 62, 732 (2010), Application(s): EIA using mouse serum and cell culture supernatant, Abstract; Full Text
Mitogen-activated protein kinase phosphatase-1 deficiency decreases atherosclerosis in apolipoprotein E null mice by reducing monocyte chemoattractant protein-1 levels: S. Imaizumi, et al.; Mol. Genet. Metab. 101, 66 (2010), Application(s): EIA using mouse serum, Abstract; Full Text
Acid sphingomyelinase involvement in tumor necrosis factor α-regulated vascular and steroid disruption during luteolysis in vivo: L.E. Henkes, et al. ; PNAS 105, 7670 (2008), Application(s): EIA using mouse tissue (ref. 1), Abstract;
Immunosuppressive Effect of Isopropanol: Down-Regulation of Cytokine Production Results from the Alteration of Discrete Transcriptional Pathways in Activated Lymphocytes: O. Desy, et al. ; J. Immunol. 181, 2348 (2008), Application(s): EIA using mouse serum, Abstract;
Leptin improves pulmonary bacterial clearance and survival in ob/ob mice during pneumococcal pneumonia: A. Hsu, et al.; Clin. Exp. Immunol. 150, 332 (2007), Application(s): EIA using mouse tissue homogenate, Abstract; Full Text

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