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IL-6 (mouse), ELISA kit

 
ADI-900-045 96 wells 447.00 USD
Do you need bulk/larger quantities?
 
  • Results in <3 hours
  • Sensitive to 1.01 pg/mL
  • Measure up to 36 samples per 96 well plate
  • Ready-to-use reagents and pre-coated plates reduce errors and save your time
The IL-6 (mouse), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours.
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Product Specification

Alternative Name:Interleukin-6
 
Sensitivity:1.01 pg/ml (range 7.81-1,000 pg/ml)
 
Assay Time:<3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of mouse Il-6 in culture supernatants and serum. Cited sample type includes tissue.
 
Species reactivity:Mouse
 
Use/Stability:Store all components at 4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 13, Wash buffer concentrate, Standard, TMB Substrate, Stop solution
 
Scientific Background:Interleukin-6 (IL-6) is a cytokine critical to the regulation of the immune and hematopoietic systems. Human IL-6 is expressed by T-cells, mast cells, monocytes, macrophages, fibroblasts, endothelial cells, keratinocytes, and many tumor cell lines. It appears to take part in acute phase reactions and response to injury and inflammation. Human IL-6 also stimulates the differentiation of B-cells for antibody production, promotes expansion of activated T-cells, expands hematopoietic cell production, and induces the expression of acute phase proteins.
 
UniProt ID:P08505
 

Product Literature References

Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages: Y.A. Bae, et al.; Korean J. Physiol. Pharmacol. 20, 415 (2016), Application(s): ELISA using culture supernatants, Abstract; Full Text
CLOCK modulates survival and acute lung injury in mice with polymicrobial sepsis: C.Y. Wang, et al.; Biochem. Biophys. Res. Commun. 478, 935 (2016), Application(s): Measurement of cytokine levels in serum and cell culture medium, Abstract;
Sphingomyelin generated by sphingomyelin synthase 1 is involved in attachment and infection with Japanese encephalitis virus: M. Taniguchi, et al.; Sci. Rep. 6, 37829 (2016), Abstract; Full Text
The inhibiting effect of the Coptis chinensis polysaccharide on the type II diabetic mice: L. Cui, et al.; Biomed. Pharmacother. 81, 111 (2016), Application(s): ELISA using mouse blood samples, Abstract;
Ginseng marc-derived low-molecular weight oligosaccharide inhibits the growth of skin melanoma cells via activation of RAW264. 7 cells: J.Y. Seo, et al.; Int. Immunopharmacol. 29, 344 (2015), Application(s): ELISA using culture supernatants, Abstract;
The immunostimulating activity of quercetin 3-O-xyloside in murine macrophages via activation of the ASK1/MAPK/NF-κB signaling pathway: J. Lee, et al.; Int. Immunopharmacol. 31, 88 (2015), Application(s): ELISA using cell culture supernatants, Abstract;
Antioxidative, anti-inflammatory, and matrix metalloproteinase inhibitory activities of 20 (S)-ginsenoside Rg3 in cultured mammalian cell lines: Y.M. Shin, et al.; Mol. Biol. Rep. 40, 269 (2013), Application(s): EIA using culture supernatants from RAW264.7 macrophage cells, Abstract;
Cilostazol enhances apoptosis of synovial cells from rheumatoid arthritis patients with inhibition of cytokine formation via Nrf2-linked heme oxygenase 1 induction: S.Y. Park, et al.; Arthritis Rheum. 62, 732 (2010), Application(s): EIA using mouse serum and cell culture supernatant, Abstract; Full Text
Leptin improves pulmonary bacterial clearance and survival in ob/ob mice during pneumococcal pneumonia: A. Hsu, et al.; Clin. Exp. Immunol. 150, 332 (2007), Application(s): EIA using mouse tissue homogenate, Abstract; Full Text

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