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Gastrin I (human), ELISA kit

 
ADI-900-026 96 wells 394.00 USD
Do you need bulk/larger quantities?
 
  • Safe - non-radioactive reagents
  • Ready-to-use - color coded reagents and pre-coated plates reduce errors
  • highly sensitive
  • Measure up to 39 samples in duplicate in 5 hours
The Gastrin I (human), EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. Ready-to-use liquid color-coded reagents are provided to reduce error in the lab.
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Product Specification

Sensitivity:7.27 pg/ml (range 39.1 - 10,000 pg/ml)
 
Assay Time:5 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of human Gastrin I in culture supernatants, plasma, and serum.
 
Species reactivity:Human
 
Crossreactivity:Gastrin I (1-17) (100%), Minigastrin (G13-I) (74.6%), rat Gastrin I (70.7%), Gastrin II (G-17-II, sulfated) (9.30%), Cholecystokinin 26-33 (CCK-8) (2.67%), Gastrin Tetrapeptide (CCK-4) (1.60%), Big Gastrin (G34-I) (0.80%) and <0.001%: Gastrin Releasing Peptide (GRP), Gastrin Inhibitory Polypeptide (GIP), Glucagon, Bombesin, Pancreatic Polypeptide, Vasoactive Intestinal Peptide (VIP), Somatostatin-14
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:Gastrins are a family of sequence-related carboxyamidated peptides produced by endocrine G cells of the antrum mucosa, and are potent stimulators of gastric acid secretion. Antral distension, partially digested proteins, amino acids, and vagal stimulation resulting from smelling, tasting, chewing or swallowing food all contribute to gastrin release from G cell storage. In addition, caffeine, alcohol, hypoglycemia, antacids and elevated calcium levels also stimulate gastrin release. Increased serum gastrin levels are associated with duodenal ulcers, Helicobacter pylori infections, colorectal carcinomas, and other tumors and cancerous lesions.
 
UniProt ID:P01350
 

Product Literature References

A high-fat diet regulates gastrin and acid secretion through primary cilia: M. Saqui-Salces, et al.; FASEB J. 26, 3127 (2012), Abstract; Full Text
Conditional deletion of menin results in antral G cell hyperplasia and hypergastrinemia: N.A. Veniaminova, et al.; American J. Physiol. Gastrointest. Liver Physiol. 303, G752 (2012), Abstract; Full Text
IL1B induced Smad 7 negatively regulates gastrin expression: D. Datta De, et al.; PLoS One 6, e14775 (2011), Application(s): Human duodenal ulcer biopsy samples, Abstract; Full Text

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