Big Endothelin-1 (human), ELISA kit



ADI-900-022	96 wells	510.00 USD

Safe - non-radioactive determination of Big ET-1
Easy-to-use - less steps compared to Western blot
Reliable - consistent results lot-after-lot

The Big Endothelin-1 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results overnight + 1 hour.

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Product Specification

Sensitivity:	0.23 pg/ml (range 0.78 - 100 pg/ml)

Kit/Set Contains:	Microtiter plate, Labeled antibody concentrate, Antibody diluent, Assay buffer, Wash buffer concentrate, Standard, TMB Substrate, Stop solution

Application:	For the quantitative determination of human Big ET-1 in culture supernatants and serum. Cited species and sample type include rabbit and lung lavage fluid (ref. 1).

Long Term Storage:	+4°C

Miscellaneous/General:	Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ET_A and ET_B. Activation of ET_A increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ET_B receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.

Product Literature References

Elevated immunoreactive endothelin-1 levels in newborn rabbit lungs after meconium aspiration: A. Zagariya, et al. ; Pediatr. Crit. Care Med. 3, 297 (2002), Application(s): EIA using rabbit lung lavage fluid (ref. 1), Abstract;

Endothelin-1 Is Induced by Cytokines in Human Vascular Smooth Muscle Cells: Evidence for Intracellular Endothelin-Converting Enzyme: M. Woods, et al. ; Mol. Pharmacol. 55, 902 (1999), Application(s): EIA using human culture supernatants, Abstract;