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Endothelin-1 ELISA kit

Sensitive, rapid Endothelin-1 ELISA kit for cardiovascular and stress-related research.
 
ADI-900-020A 96 wells 515.00 USD
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  • Highly sensitive measurement, quantify as little as 0.41 pg/ml ET-1, allowing you read both normal and pathological samples
  • Higher throughput format with results in just 2 hours for up to 38 samples in duplicate
  • Flexible option of overnight or 1 hour primary incubation
  • Suitable for a variety of sample matrices
The Endothelin-1 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 2 hours. This ultra-sensitive kit lets you quantify as little as 0.41 pg/mL ET-1 in a variety of biological matrices with the option of overnight or a 1 hour primary incubation.
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Product Specification

Sensitivity:0.40 pg/ml (range 0.78 - 100 pg/ml)
 
Assay Time:2 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of ET-1 in culture supernatants, plasma, and serum of human, mouse, rat, cow, dog, pig, and rabbit origin. Cited sample types include cell lysates and tissue homogenates.
 
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Porcine, Rabbit
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:Microtiter plate, Antibody diluent, Antibody concentrate, Assay buffer 17, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2
 
Scientific Background:Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ETA and ETB. Activation of ETA increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ETB receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.
 
UniProt ID:P05305
 

Product Literature References

Cardiac volume overload and pulmonary hypertension in long-term follow-up of patients with a transjugular intrahepatic portosystemic shunt: A. Wannhoff, et al.; Aliment. Pharmacol. Ther. 43, 955 (2016), Application(s): Measured peripheral blood levels of endothelin-1 (ET-1), Abstract; Full Text
Grape seed flavanols decrease blood pressure via Sirt-1 and confer a vasoprotective pattern in rats: Z. Pons, et al.; J. Funct. Foods 24, 164 (2016), Application(s): Plasma ET-1 was measured,
Hyperglycemia Induces Cellular Hypoxia through Production of Mitochondrial ROS Followed by Suppression of Aquaporin-1: K. Sada, et al.; PLoS One 11, e0158619 (2016), Application(s): Secreted endothelin-1 levels in the culture supernatants, Abstract; Full Text
Relationships between Cognitive Function and Cerebral Blood Flow, Oxidative Stress and Inflammation, in Older Heart Failure Patients: C.E. Kure, et al.; J. Card. Fail. 22, 548 (2016), Application(s): Measurement of endothelin-1 levels in plasma samples, Abstract;
Hydrogen Sulphide as a Novel Therapy to Reduce the Nephrotoxic Effects of Calcineurin Inhibitors: G. Lee, et al.; J. Surg. Res. 197, 419 (2015), Application(s): ELISA using pig urine, Abstract;
Effects of vascular endothelial growth factor on endothelin-1 production by human lung microvascular endothelial cells in vitro: G.P. Star, et al.; Life Sci. 118, 191 (2014), Application(s): ELISA using human cell culture supernatant, Abstract;
Hypoxic treatment of human dual placental perfusion induces a preeclampsia-like inflammatory response: A. Jain, et al.; Lab. Invest. 94, 873 (2014), Application(s): ELISA using hypoxia and normoxia blood samples, Abstract;
miRNA-1 regulates endothelin-1 in diabetes: B. Feng, et al.; Life Sci. 98, 18 (2014), Application(s): ELISA using culture supernatants, Abstract;
Water deprivation induces neurovascular and cognitive dysfunction through vasopressin-induced oxidative stress: G. Faraco, et al.; J. Cereb. Blood Flow Metab. 34, 852 (2014), Application(s): ELISA using mouse tissue homogenates, Abstract; Full Text
Acute effects of red wine on cytochrome P450 eicosanoids and blood pressure in men: A.E. Barden, et al.; J. Hypertens. 31, 2195 (2013), Application(s): ELISA, Abstract; Full Text
Altered expression of enzymes regulating the activity of endothelin-1 in the lower segment of the human amnion during labor: T. Kotani, et al.; Biol. Reprod. 89, 52 (2013), Application(s): EIA using culture supernatants, Abstract;
Effects of Glucose Concentration on Propofol Cardioprotection against Myocardial Ischemia Reperfusion Injury in Isolated Rat Hearts: X. Yao, et al.; FASEB J. 27 (Meeting Abstract Supplement), lb620 (2013), Application(s): ELISA, Full Text
Cardiomyocyte-specific overexpression of HEXIM1 prevents right ventricular hypertrophy in hypoxia-induced pulmonary hypertension in mice: N. Yoshikawa, et al.; PLoS One 7, e52522 (2012), Application(s): ELISA using mouse plasma, Abstract; Full Text
Correlation of endothelin-1 concentration in aqueous humor with intraocular pressure in primary open angle and pseudoexfoliation glaucoma: L. Choritz, et al.; Invest. Ophthalmol. Vis. Sci. 53, 7336 (2012), Application(s): ELISA using human aqueous humor and blood samples, Abstract;
A novel role of endothelin-1 in linking Toll-like receptor 7-mediated inflammation to fibrosis in congenital heart block: D. Alvarez, et al.; J. Biol. Chem. 286, 30444 (2011), Application(s): ELISA using mouse cell culture supernatants, Abstract; Full Text
dsRNA activation of endothelin-1 and markers of vascular activation in endothelial cells and fibroblasts: G. Farina, et al.; Ann. Rheum. Dis. 70, 544 (2011), Application(s): ELISA using human dermal microvascular endothelial cell and pulmonary artery endothelial cell lysates, primary human dermal fibroblast explant culture supernatants and mouse serum, Abstract; Full Text
BMPR2 mutation alters the lung macrophage endothelin-1 cascade in a mouse model and patients with heritable pulmonary artery hypertension: M. Talati, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 299, L363 (2010), Application(s): ELISA using human macrophage cell culture supernatants, Abstract; Full Text
Green tea polyphenol epigallocatechin gallate reduces endothelin-1 expression and secretion in vascular endothelial cells: roles for AMP-activated protein kinase, Akt, and FOXO1: C. E. Reiter, et al.; Endocrinology 151, 103 (2010), Application(s): ELISA using primary bovine and human aortic endothelial cell lysates and culture supernatants, Abstract; Full Text
Endothelin-1 and transforming growth factor-beta1 independently induce fibroblast resistance to apoptosis via AKT activation: P. Kulasekaran, et al.; Am. J. Respir. Cell Mol. Biol. 41, 484 (2009), Application(s): ELISA using primary human lung fibroblast cell culture supernatants, Abstract; Full Text
Endothelin-1 regulates oligodendrocyte development: A. Gadea, et al.; J. Neurosci. 29, 10047 (2009), Application(s): ELISA using rat astrocyte-conditioned medium, Abstract; Full Text
Endothelin-1 mediates hypoxia-induced inhibition of voltage-gated K+ channel expression in pulmonary arterial myocytes: E. M. Whitman, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 294, L309 (2008), Application(s): ELISA using rat pulmonary arterial smooth muscle cell culture supernatants, Abstract; Full Text
Transgenic expression of a dominant negative K(ATP) channel subunit in the mouse endothelium: effects on coronary flow and endothelin-1 secretion: B. Malester, et al.; FASEB J. 21, 2162 (2007), Application(s): ELISA using primary human coronary artery endothelial cell culture supernatants, Abstract; Full Text
Dehydroepiandrosterone mimics acute actions of insulin to stimulate production of both nitric oxide and endothelin 1 via distinct phosphatidylinositol 3-kinase- and mitogen-activated protein kinase-dependent pathways in vascular endothelium: G. Formoso, et al.; Mol. Endocrinol. 20, 1153 (2006), Application(s): ELISA using primary bovine aortic endothelial cell culture supernatants, Abstract; Full Text

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