Highly sensitive measurement, quantify as little as 0.41 pg/ml ET-1, allowing you read both normal and pathological samples
Higher throughput format with results in just 2 hours for up to 38 samples in duplicate
Flexible option of overnight or 1 hour primary incubation
Suitable for a variety of sample matrices
The Endothelin-1 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 2 hours. This ultra-sensitive kit lets you quantify as little as 0.41 pg/mL ET-1 in a variety of biological matrices with the option of overnight or a 1 hour primary incubation.
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Product Details
Sensitivity:
0.41 pg/ml (range 0.78 - 100 pg/ml)
Assay Time:
2 hours
Applications:
ELISA, Colorimetric detection
Application Notes:
For the quantitative determination of ET-1 in culture supernatants, plasma, and serum of human, mouse, rat, cow, dog, pig, and rabbit origin. Cited sample types include cell lysates and tissue homogenates.
Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ETA and ETB. Activation of ETA increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ETB receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.
NiONPs-induced alteration in calcium signaling and mitochondrial function in pulmonary artery endothelial cells involves oxidative stress and TRPV4 channels disruption: O. Germande, et al.; Nanotoxicology 16, 29 (2022), Abstract;
Treatment with apocynin selectively restores hippocampal arteriole function and seizure-induced hyperemia in a model of preeclampsia: M.J. Cipolla, et al.; J. Cereb. Blood Flow Metab. 42, 8 (2022), Abstract;
Vasorin plays a critical role in vascular smooth muscle cells and arterial function: L. Louvet, et al.; J. Cell. Physiol. 237, 3845 (2022), Abstract;
Contributions of Sodium-Hydrogen Exchanger-1 and Mitogen-Activated Protein Kinases to Enhanced Retinal Venular Constriction to Endothelin-1 in Diabetes: Y.L. Chen, et al.; Diabetes 70, 2353 (2021), Abstract;
Dehulled Adlay Consumption Modulates Blood Pressure in Spontaneously Hypertensive Rats and Overweight and Obese Young Adults: W.-J. Jeh, et al.; Nutrients 13, 2305 (2021), Abstract;
Estrogen modulates metabolic risk profile after resistance training in early postmenopausal women: a randomized controlled trial: T.V. Dam, et al.; Menopause 28, 1214 (2021), Abstract;
Impaired pulmonary vasomotor control in exercising swine with multiple comorbidities: J. van de Wouw, et al.; Basic Res. Cardiol. 116, 51 (2021), Abstract;
Anthocyanin-Rich New Zealand Blackcurrant Extract Supports the Maintenance of Forearm Blood-Flow During Prolonged Sedentary Sitting: M.J. Barnes, et al.; Front. Nutr. 7, 74 (2020), Abstract; Full Text
Aerobic exercise training improves insulin-induced vasorelaxation in a vessel-specific manner in rats with insulin-treated experimental diabetes: M.W. McDonald, et al.; Diab. Vasc. Dis. Res. 16, 77 (2019), Application(s): ELISA using rat serum, Abstract;
Chronic consumption of a low calorie, high polyphenol cranberry beverage attenuates inflammation and improves glucoregulation and HDL cholesterol in healthy overweight humans: a randomized controlled trial: B. Chew, et al.; Eur. J. Nutr. 58, 1223 (2019), Application(s): Human blood plasma, Abstract; Full Text
Gelatin-based perfusable, endothelial carotid artery model for the study of atherosclerosis: R. Chen, et al.; Biomed. Eng. Online 18, 87 (2019), Abstract; Full Text
Hyperglycemia enhances constriction of retinal venules via activation of the reverse-mode sodium-calcium exchanger: Y.L. Chen, et al.; Diabetes 68, 1624 (2019), Application(s): ELISA using pig vitreous humor, Abstract;
In Vivo Comparative Study on Acute and Sub-acute Biological Effects Induced by Ultrafine Particles of Different Anthropogenic Sources in BALB/c Mice: F. Farina, et al.; Int. J. Mol. Sci. 20, 2805 (2019), Application(s): Cell culture supernatants, Abstract; Full Text
Co-ingestion of whole eggs or egg whites with glucose protects against postprandial hyperglycaemia-induced oxidative stress and dysregulated arginine metabolism in association with improved vascular endothelial function in prediabetic men: J.D. McDonald, et al.; Br. J. Nutr. 120, 901 (2018), Application(s): Human blood plasma, Abstract;
Effects of Personal Short-Term Exposure to Ambient Ozone on Blood Pressure and Vascular Endothelial Function: A Mechanistic Study Based on DNA Methylation and Metabolomics: Y. Xia, et al.; Environ. Sci. Technol. 52, 12774 (2018), Application(s): Human blood serum, Abstract;
Andrographolide inhibits hypoxia-induced HIF-1α-driven endothelin 1 secretion by activating Nrf2/HO-1 and promoting the expression of prolyl hydroxylases 2/3 in human endothelial cells: H.C. Lin, et al.; Environ. Toxicol. 32, 918 (2017), Application(s): Medium supernatant from human A.hy926 cells, Abstract;
Renoprotective effects of montelukast in an experimental model of cisplatin nephrotoxicity in rats: A.M. Gad, et al.; J. Biochem. Mol. Toxicol. 31, (2017), Application(s): Rat serum and renal lysates, Abstract;
Cardiac volume overload and pulmonary hypertension in long-term follow-up of patients with a transjugular intrahepatic portosystemic shunt: A. Wannhoff, et al.; Aliment. Pharmacol. Ther. 43, 955 (2016), Application(s): Measured peripheral blood levels of endothelin-1 (ET-1), Abstract; Full Text
Grape seed flavanols decrease blood pressure via Sirt-1 and confer a vasoprotective pattern in rats: Z. Pons, et al.; J. Funct. Foods 24, 164 (2016), Application(s): Plasma ET-1 was measured,
Hyperglycemia Induces Cellular Hypoxia through Production of Mitochondrial ROS Followed by Suppression of Aquaporin-1: K. Sada, et al.; PLoS One 11, e0158619 (2016), Application(s): Secreted endothelin-1 levels in the culture supernatants, Abstract; Full Text
Relationships between Cognitive Function and Cerebral Blood Flow, Oxidative Stress and Inflammation, in Older Heart Failure Patients: C.E. Kure, et al.; J. Card. Fail. 22, 548 (2016), Application(s): Measurement of endothelin-1 levels in plasma samples, Abstract;
Hydrogen Sulphide as a Novel Therapy to Reduce the Nephrotoxic Effects of Calcineurin Inhibitors: G. Lee, et al.; J. Surg. Res. 197, 419 (2015), Application(s): ELISA using pig urine, Abstract;
Effects of vascular endothelial growth factor on endothelin-1 production by human lung microvascular endothelial cells in vitro: G.P. Star, et al.; Life Sci. 118, 191 (2014), Application(s): ELISA using human cell culture supernatant, Abstract;
Ellagic acid antiinflammatory and antiapoptotic potential mediate renoprotection in cisplatin nephrotoxic rats: A.M. El-Garhy, et al.; J. Biochem. Mol. Toxicol. 28, 472 (2014), Application(s): Rat serum and renal lysates, Abstract;
Hypoxic treatment of human dual placental perfusion induces a preeclampsia-like inflammatory response: A. Jain, et al.; Lab. Invest. 94, 873 (2014), Application(s): ELISA using hypoxia and normoxia blood samples, Abstract;
miRNA-1 regulates endothelin-1 in diabetes: B. Feng, et al.; Life Sci. 98, 18 (2014), Application(s): ELISA using culture supernatants, Abstract;
Serine Carboxypeptidase SCPEP1 and Cathepsin A Play Complementary Roles in Regulation of Vasoconstriction via Inactivation of Endothelin-1: X. Pan, et al.; PLoS Genet. 10, e1004146 (2014), Abstract; Full Text
Water deprivation induces neurovascular and cognitive dysfunction through vasopressin-induced oxidative stress: G. Faraco, et al.; J. Cereb. Blood Flow Metab. 34, 852 (2014), Application(s): ELISA using mouse tissue homogenates, Abstract; Full Text
Acute effects of red wine on cytochrome P450 eicosanoids and blood pressure in men: A.E. Barden, et al.; J. Hypertens. 31, 2195 (2013), Application(s): ELISA, Abstract; Full Text
Altered expression of enzymes regulating the activity of endothelin-1 in the lower segment of the human amnion during labor: T. Kotani, et al.; Biol. Reprod. 89, 52 (2013), Application(s): EIA using culture supernatants, Abstract;
Effects of Glucose Concentration on Propofol Cardioprotection against Myocardial Ischemia Reperfusion Injury in Isolated Rat Hearts: X. Yao, et al.; FASEB J. 27 (Meeting Abstract Supplement), lb620 (2013), Application(s): ELISA, Full Text
Hypoxia/reoxygenation stress increases markers of vaso-occlusive crisis in sickle SAD mice: E. Aufradet, et al.; Clin. Hemorheol. Microcirc. 54, 297 (2013), Application(s): Mouse Plasma, Abstract;
Cardiomyocyte-specific overexpression of HEXIM1 prevents right ventricular hypertrophy in hypoxia-induced pulmonary hypertension in mice: N. Yoshikawa, et al.; PLoS One 7, e52522 (2012), Application(s): ELISA using mouse plasma, Abstract; Full Text
Correlation of endothelin-1 concentration in aqueous humor with intraocular pressure in primary open angle and pseudoexfoliation glaucoma: L. Choritz, et al.; Invest. Ophthalmol. Vis. Sci. 53, 7336 (2012), Application(s): ELISA using human aqueous humor and blood samples, Abstract;
A novel role of endothelin-1 in linking Toll-like receptor 7-mediated inflammation to fibrosis in congenital heart block: D. Alvarez, et al.; J. Biol. Chem. 286, 30444 (2011), Application(s): ELISA using mouse cell culture supernatants, Abstract; Full Text
dsRNA activation of endothelin-1 and markers of vascular activation in endothelial cells and fibroblasts: G. Farina, et al.; Ann. Rheum. Dis. 70, 544 (2011), Application(s): ELISA using human dermal microvascular endothelial cell and pulmonary artery endothelial cell lysates, primary human dermal fibroblast explant culture supernatants and mouse serum, Abstract; Full Text
BMPR2 mutation alters the lung macrophage endothelin-1 cascade in a mouse model and patients with heritable pulmonary artery hypertension: M. Talati, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 299, L363 (2010), Application(s): ELISA using human macrophage cell culture supernatants, Abstract; Full Text
Green tea polyphenol epigallocatechin gallate reduces endothelin-1 expression and secretion in vascular endothelial cells: roles for AMP-activated protein kinase, Akt, and FOXO1: C. E. Reiter, et al.; Endocrinology 151, 103 (2010), Application(s): ELISA using primary bovine and human aortic endothelial cell lysates and culture supernatants, Abstract; Full Text
Endothelin-1 and transforming growth factor-beta1 independently induce fibroblast resistance to apoptosis via AKT activation: P. Kulasekaran, et al.; Am. J. Respir. Cell Mol. Biol. 41, 484 (2009), Application(s): ELISA using primary human lung fibroblast cell culture supernatants, Abstract; Full Text
Endothelin-1 regulates oligodendrocyte development: A. Gadea, et al.; J. Neurosci. 29, 10047 (2009), Application(s): ELISA using rat astrocyte-conditioned medium, Abstract; Full Text
Endothelin-1 mediates hypoxia-induced inhibition of voltage-gated K+ channel expression in pulmonary arterial myocytes: E. M. Whitman, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 294, L309 (2008), Application(s): ELISA using rat pulmonary arterial smooth muscle cell culture supernatants, Abstract; Full Text
Transgenic expression of a dominant negative K(ATP) channel subunit in the mouse endothelium: effects on coronary flow and endothelin-1 secretion: B. Malester, et al.; FASEB J. 21, 2162 (2007), Application(s): ELISA using primary human coronary artery endothelial cell culture supernatants, Abstract; Full Text
Dehydroepiandrosterone mimics acute actions of insulin to stimulate production of both nitric oxide and endothelin 1 via distinct phosphatidylinositol 3-kinase- and mitogen-activated protein kinase-dependent pathways in vascular endothelium: G. Formoso, et al.; Mol. Endocrinol. 20, 1153 (2006), Application(s): ELISA using primary bovine aortic endothelial cell culture supernatants, Abstract; Full Text
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