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Leptin (mouse), ELISA kit

 
ADI-900-019A 96 wells 394.00 USD
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  • Flexible - broad dynamic range suitable for many normal and pathological samples
  • Sensitive - measure < 26 pg/ml mouse leptin
  • Fast - fully quantitative results in just 3 hours from up to 39 samples in duplicate
  • Pre-coated plates and ready-to-use reagents save you time
The Leptin (mouse), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. The broad dynamic range is suitable for many normal and pathological samples with sensitive quantification of < 26 pg/mL mouse leptin.
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Product Specification

Alternative Name:OB gene product
 
Sensitivity:25.4 pg/ml (range 50 - 3,200 pg/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of mouse leptin in culture supernatants, plasma, and serum. Cited sample type includes tissue.
 
Species reactivity:Mouse
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:Microtiter plate, Standard, Antibody, Conjugate, Assay buffer 28, TMB Substrate, Stop solution 2, Wash buffer concentrate
 
Scientific Background:The adipokine leptin is a hormone secreted predominantly by adipose tissue that signals through leptin receptors in the hypothalamus to decrease appetite and increase energy expenditure. Binding of leptin to the long-form of the leptin receptor in the hypothalamus reduces neuropeptide Y (NPY) and agouti-related protein (AgRP) activity, while stimulating anorexigenic pro-opiomelanocortin (POMC) neuron activity to reduce appetite. In peripheral tissues, leptin antagonizes insulin signaling, increases fatty acid oxidation, decreases insulin production in pancreatic β cells, and promotes fertility. Its expression in adipocytes can be regulated in a paracrine fashion by other adipokines such as IL-6 (stimulation) and TNF-α (inhibition).

Leptin deficient mice display severe insulin resistance, obesity, and decreased fertility, all of which are reversible by administration of exogenous leptin. In contrast, elevated circulating levels of leptin are associated with increased obesity, indicative of an acclimated state of leptin resistance which is not well understood. Evidence also links leptin to cardiovascular disease, as it mediates numerous pro-inflammatory and pro-atherogenic effects such as increased endothelin-1 (ET-1) expression, accumulation of reactive oxygen species (ROS), secretion of monocyte chemoattractant protein-1 (MCP-1), and increased sympathetic vascular tone and blood pressure.
 
UniProt ID:P41160
 

Product Literature References

High fat diet augments amphetamine sensitization in mice: Role of feeding pattern, obesity, and dopamine terminal changes: S. C. Fordahl, et al. ; Neuropharmacology 109, 170 (2016), Application(s): Leptin mouse plasma hormones were measured, Abstract;
Increased apoptosis and browning of TAK1-deficient adipocytes protects against obesity: A. Sassmann-Schweda, et al.; JCI Insight (2016), Application(s): Measured mouse plasma levels, Full Text
Nucleus accumbens cocaine-amphetamine regulated transcript mediates food intake during novelty conflict: P.R. Burghardt, et al.; Physiol. Behav. 158, 76 (2016), Application(s): Levels of leptin were measured in blood samples, Abstract;
The NLRP3 inflammasome is dispensable for ER stress-induced pancreatic β-cell damage in Akita mice: J. Wang, et al.; Biochem. Biophys. Res. Commun. 466, 300 (2015), Application(s): ELISA kit measuring plasma leptin levels in mice, Abstract;
Adipogenic human adenovirus-36 reduces leptin expression and secretion and increases glucose uptake by fat cells: S.D. Vangipuram, et al.; Int. J. Obes. (Lond.) 31, 87 (2007), Application(s): EIA using cell culture supernatant, Abstract; Full Text
Leptin improves pulmonary bacterial clearance and survival in ob/ob mice during pneumococcal pneumonia: A. Hsu, et al.; Clin. Exp. Immunol. 150, 332 (2007), Application(s): EIA using mouse tissue homogenate, Abstract; Full Text
Folliculogenesis is impaired and granulosa cell apoptosis is increased in leptin-deficient mice: M.L. Hamm, et al.; Biol. Reprod. 71, 66 (2004), Application(s): EIA using mouse serum, Abstract; Full Text
Does leptin mediate the effect of photoperiod on immune function in mice?: G.K. Bhat, et al.; Biol. Reprod. 69, 30 (2003), Application(s): EIA using mouse serum, Abstract; Full Text

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