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Substance P ELISA kit

Highly sensitive Substance P ELISA kit enabling detection of Substance P in plasma, serum, saliva, urine, and supernatants in just 3 hours.
 
ADI-900-018 96 wells 372.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-901-018 (5x96 wells)
  • Highly sensitive assay, measuring as little as 8.04 pg/ml of Substance P
  • Widely cited in numerous peer reviewed publications
  • High throughput fomat, assay up to 38 samples in duplicate per 96 well kit
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
The Substance P EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. This kit utilized low sample volume and provides the ability to assay up to 38 samples in duplicate per 96 well kit.
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Product Specification

Sensitivity:8.04 pg/ml (range 9.76 - 10,000 pg/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of Substance P in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample type includes tissue.
 
Species reactivity:Species independent
 
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:Substance P is an undecapeptide hormone (RPKPQQFFGLM) that mediates a number of biological activities including nausea, anxiety, pain, and vasodilation. It is a member of the tachykinin family of neuropeptides, and is widely distributed in the peripheral and central nervous systems of vertebrates.
 
UniProt ID:P20366
 

Product Literature References

Effects on breathing of agonists to μ-opioid or GABAA receptors dialyzed into the Ventral Respiratory Column of awake and sleeping goats: T.M. Langer, et al.; Respir. Physiol. Neurobiol. 239, 10 (2017), Abstract;
Acid-Sensing Ion Channel 1a Contributes to Airway Hyperreactivity in Mice: L.R. Reznikov, et al.; PLoS One 11, e0166089 (2016), Abstract; Full Text
Efficacy of oral meloxicam suspension for prevention of pain and inflammation following band and surgical castration in calves: M. Olson, et al.; BMC Vet. Res. 12, 102 (2016), Application(s): Measurment of substance P in blood samples, Abstract; Full Text
Evolution of Chronic Pain in Sickle Cell Disease: M. Pope, et al.; Blood 128, 1297 (2016), Full Text
Substance P Receptor Signaling Mediates Doxorubicin-Induced Cardiomyocyte Apoptosis Triple-Negative Breast Cancer Chemoresistance: P. Robinson, et al.; Biomed. Res. Int. 2016, Article ID 1959270 (2016), Application(s): Quantitation of SP Protein in cell lysates, Abstract; Full Text
The role of substance P in the maintenance of colonic hypermotility induced by repeated stress in rats: P. Lu, et al.; Neuropeptides 56, 75 (2016), Application(s): Determine levels of substance P in serum, Abstract;
Blockade of neurokinin-1 receptors in the ventral respiratory column does not affect breathing but alters neurochemical release: C. Muere, et al.; J. Appl. Physiol. 118, 732 (2015), Application(s): ELISA, Abstract;
Characteristics and Potential Biomarkers for Chronic Pain in Patients with Sickle Cell Disease: N. Kuei, et al.; Blood 126, 986 (2015), Application(s): Human Substance P levels were measured, Full Text
Effect of anti-NGF antibodies in a rat tibia fracture model of complex regional pain syndrome type I : I. Sabsovich, et al. ; Pain 138, 47 (2008), Application(s): EIA using tissue, Abstract;
Endogenous ATP potentiates only vasopressin secretion from neurohypophysial terminals: J. Lemos, et al. ; J. Cell. Physiol. 217, 155 (2008), Application(s): EIA using rat culture supernatants, Abstract;
Tachykinin neurokinin 3 receptor signaling in cholecystokinin-elicited release of oxytocin and vasopressin: F. Flynn, et al. ; Am. J. Physiol. Regul. Integr. Comp. Physiol. 294, R1760 (2008), Application(s): EIA using rat plasma, Abstract; Full Text
Loss of Vav2 proto-oncogene causes tachycardia and cardiovascular disease in mice: X. Bustelo, et al. ; Mol. Biol. Cell 18, 943 (2007), Application(s): EIA using mouse plasma, Abstract;
Tachykinin NK3 receptor contribution to systemic release of vasopressin and oxytocin in response to osmotic and hypotensive challenge: G.E. Haley, et al. ; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R931 (2007), Application(s): EIA using rat plasma, Abstract; Full Text
Urea transporter UT-A1 and aquaporin-2 proteins decrease in response to angiotensin II or norepinephrine-induced acute hypertension: J.D. Klein, et al. ; Ren. Physiol. 291, F952 (2006), Application(s): EIA using rat plasma, Abstract;

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