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Arg8-Vasopressin ELISA kit

 
ADI-900-017 96 wells 370.00 USD
 
Alternative size available: ADI-901-017 (5x96 wells)
  • Trusted - cited in numerous peer-reviewed journals for use in a variety of sample types including urine, perfusates, plasma, tissue, and serum
  • Simple - liquid color-coded reagents reduce error
  • Specific - negligible reactivity with related molecules
The Arg8-Vasopressin EIA kit is a colorimetric competitive enzyme immunoassay kit with results overnight 1 hour.. Absorbance is read at 405 nm. Document your results to scientists worldwide, this kit has been cited in numerous peer-reviewed journals for use in a variety of sample types including urine, perfusates, plasma, tissue, and serum. The simple liquid color-coded reagents reduce error.
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Product Specification

Alternative Name:AVP
 
Sensitivity:3.39 pg/ml (range 4.10 - 1,000 pg/ml)
 
Assay Time:Overnight + 1 hour
 
Application:For the quantitative determination of Arg8-Vasopressin in culture supernatants from any species. Cited sample type includes plasma (ref. 1, 2), serum and urine.
 
Crossreactivity:Arg8-Vasopressin (100%), Lys8-Vasopressin (7.30%) and <0.001%: Oxytocin, TRH, VIP, Leu-Enkephalin, Met-Enkephalin, Mesotocine, Somatostatin, Vasotocin, Desmopressin, Arg8-Vasotocin, Ser4-Ile8-Oxytocin
 
Use/Stability:Store all components at 4°, except standard and conjugate at -20°.
 
Kit/Set Contains:GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Miscellaneous/General:Arginine vasopressin (AVP) is a nonapeptide hormone produced in magnocellular neurons of the hypothalamus, which functions as an antidiuretic in the kidney and systemically as a regulator of vasoconstriction, blood volume, and blood pressure. AVP acts by binding to a set of specific seven transmembrane domain-containing G-protein coupled receptors (GPCR) classified into three groups: V1a (V1), V1b (V3), and V2. V1b receptors stimulate adrenocorticotropic hormone (ACTH) release in the anterior pituitary, but may also participate in corticotropin-releasing hormone (CRH) release by the hypothalamus. The V1b receptor signals through coupling of Gq/11 alpha subunits, activating phospholipase C and release of intracellular Ca2+ via phosphoinositide second messengers. V1b receptor overexpression is a common marker for ACTH-secreting corticotropic adenomas. V2 receptors in the collecting ducts of the kidneys stimulate adenylyl cyclase activity, regulating the reabsorption of water via aquaporin channels.
 
UniProt ID:P01185 (human)
 

Product Literature References

Excess maternal salt intake produces sex-specific hypertension in offspring: putative roles for kidney and gastrointestinal sodium handling: C. Gray, et al.; PLoS One 8, e72682 (2013), Application(s): EIA using rat plasma, Abstract; Full Text
Peripheral vasopressin but not oxytocin relates to severity of acute psychosis in women with acutely-ill untreated first-episode psychosis: L.H. Rubin, et al.; Schizophr. Res. 146, 138 (2013), Application(s): ELISA using human serum., Abstract;
Defective renal water handling in transgenic mice over-expressing human CD39/NTPDase1: Y. Zhang, et al.; Am. J. Physiol. Renal Physiol. 303, F420 (2012), Application(s): ELISA using mouse urine, Abstract;
Oxytocin and vasopressin are dysregulated in Williams Syndrome, a genetic disorder affecting social behavior: L. Dai, et al.; PLoS One 7, e38513 (2012), Application(s): ELISA using human serum, Abstract; Full Text
Physiological function of the angiotensin AT1a receptor in bone remodeling: K. Kaneko, et al.; J. Bone Miner. Res. 26, 2959 (2011), Application(s): ELISA using mouse serum, Abstract;
Prolactin promotes oxytocin and vasopressin release by activating neuronal nitric oxide synthase in the supraoptic and paraventricular nuclei: C. Vega, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 299, R1701 (2010), Application(s): ELISA using rat plasma, Abstract; Full Text
Endogenous ATP potentiates only vasopressin secretion from neurohypophysial terminals: J. Lemos, et al. ; J. Cell. Physiol. 217, 155 (2008), Application(s): EIA using rat culture supernatants, Abstract;
Modulation of aquaporin-2/vasopressin2 receptor kidney expression andtubular injury after endotoxin (lipopolysaccharide) challenge: F. Chagnon, et al.; Crit. Care Med. 36, 3054 (2008), (ref. 1),
Tachykinin neurokinin 3 receptor signaling in cholecystokinin-elicited release of oxytocin and vasopressin: F. Flynn, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 294, R1760 (2008), Application(s): EIA using rat plasma, Abstract;
Hormonal correlates of human paternal interactions: A hospital-based investigation in urban Jamaica: P.B. Gray, et al.; Horm. Behav. 52, 499 (2007), Application(s): EIA using human urine, Abstract;
Loss of Vav2 proto-oncogene causes tachycardia and cardiovascular disease in mice: X. Bustelo, et al. ; Mol. Biol. Cell 18, 943 (2007), Application(s): EIA using mouse plasma, Abstract;
Tachykinin NK3 receptor contribution to systemic release of vasopressin and oxytocin in response to osmotic and hypotensive challenge: G.E. Haley, et al. ; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R931 (2007), Application(s): EIA using rat plasma (ref. 2), Abstract;
Urea transporter UT-A1 and aquaporin-2 proteins decrease in response to angiotensin II or norepinephrine-induced acute hypertension: J.D. Klein, et al. ; Ren. Physiol. 291, F952 (2006), Application(s): EIA using rat plasma, Abstract;

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