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Direct cGMP ELISA kit

Most sensitive (0.025 pmol/ml) colorimetric kit for intracellular cGMP quantification.
 
ADI-900-014 96 wells 360.00 USD
 
Replaces Prod. #: BML-AK300

Alternative size available: ADI-901-014 (5x96 wells)
  • Easy-to-use – pre-coated plates and liquid color-coded reagents reduce errors
  • Sensitive – optional acetylation protocol increases sensitivity 10-fold (0.025 pmol/ml)
  • Trusted – regularly cited in peer-reviewed publications
  • Higher throughput – obtain results from up to 39 samples in duplicate per 96 well plate
  • Simple – efficient and well-established sample handling protocols
  • Reliable – consistent lot-to-lot performance
This is a colorimetric competitive immunoassay kit for the quantitation of intracellular cGMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm.The ready-to-use liquid color-coded reagents provided reduce error.
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Product Specification

Alternative Name:cyclic GMP
 
Sensitivity:(non-acetylated) 0.604 pmol/ml; (acetylated) 0.025 pmol/ml (range (non-acetylated) 0.8 - 500 pmol/ml; (acetylated) 0.08 - 50 pmol/ml)
 
Assay Time:2 hours
 
Application:For the quantitative determination of cGMP in cell lysates and tissue from any species. Cited sample types include cerebral spinal fluid (ref. 1) and microdialysate (ref. 2).
 
Crossreactivity:cyclic GMP (100%) and <0.001% GMP, GTP, cyclic AMP, AMP, ATP, cyclic UMP, CTP
 
Use/Stability:Store all components at 4°, except standard and conjugate at -20°.
 
Kit/Set Contains:GxR IgG Microtiter plate, Conjugate, Antibody, 0.1M HCl, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine
 
Miscellaneous/General:Cyclic GMP (cGMP), which has been shown to be present at levels typically 10-100 fold lower than cAMP, is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones, such as Acetylcholine, Insulin, and Oxytocin, as well as certain other chemicals like Serotonin and Histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate, and nitric oxide (NO) also stimulate cGMP levels.
 

Product Literature References

β-Sitosterol oxidation products attenuate vasorelaxation by increasing reactive oxygen species and cyclooxygenase-2: C. Yang, et al.; Cardiovasc. Res. 97, 520 (2013), Application(s): ELISA using rat aortic tissue, Abstract; Full Text
Chronic deficiency of nitric oxide affects hypoxia inducible factor-1α (HIF-1α) stability and migration in human endothelial cells: M.G. Cattaneo, et al.; PLoS One 6, e29680 (2011), Application(s): EIA using human cell lysate, Abstract; Full Text
cGMP secreted from the tapeworm Hymenolepis diminuta is a signal molecule to the host intestine: N. Zimmerman, et al. ; J. Parasitol. 94, 771 (2008), Application(s): EIA using tapeworm culture supernatants, Abstract;
Evidence for functional heterogeneity of circulating B-type natriuretic peptide: A. Protter, et al. ; J. Am. Coll. Cardiol. 49, 1071 (2007), Application(s): EIA using human cell lysates, Abstract;
Estradiol and progesterone modulate the nitric oxide/cyclic gmp pathway in the hypothalamus of female rats and in GT1-1 cells: H.P. Chu, et al. ; Endocrine 24, 177 (2004), Application(s): EIA using rat cell lysates, Abstract;
A Novel PDZ Protein Regulates the Activity of Guanylyl Cyclase C, the Heat-stable Enterotoxin Receptor: R.O. Scott, et al. ; J. Biol. Chem. 277, 22934 (2002), Application(s): EIA using monkey cell lysates, Abstract;
The Mechanism of Ciliary Stimulation by Acetylcholine: Roles of Calcium, PKA, and PKG: O. Zagoory, et al. ; J. Gen. Physiol. 119, 329 (2002), Application(s): EIA using frog cell lysates, Abstract;
Differential nitric oxide synthase activity, cofactor availability and cGMP accumulation in the central nervous system during anaesthesia: H.F. Galley, et al. ; Br. J. Anaesth. 86, 388 (2001), Application(s): EIA using rat cell lysates, Abstract;
Mechanism underlying diuretic effect of L-NAME at a subpressor dose: M. Liang, et al. ; Ren. Physiol. 281, F414 (2001), Application(s): EIA using rat microdialysate; (ref. 2), Abstract;
The plasma membrane calmodulin-dependent calcium pump: a major regulator of nitric oxide synthase I: K. Schuh, et al. ; J. Cell Biol. 155, 201 (2001), Application(s): EIA using human cell lysates, Abstract;
Role of cGMP versus 20-HETE in the vasodilator response to nitric oxide in rat cerebral arteries: C.W. Sun, et al. ; Am. J. Physiol. Heart Circ. Physiol. 279, H339 (2000), Application(s): EIA using rat cell lysates, Abstract;
Increased cerebrospinal fluid cAMP levels in Alzheimer's disease: M. Martinez, et al. ; Brain Res. 846, 265 (1999), Application(s): EIA using human cerbrospinal fluid; (ref. 1), Abstract;

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