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Progesterone ELISA kit

ADI-900-011 96 wells 295.00 USD
Do you need bulk/larger quantities?
Alternative size available: ADI-901-011 (5x96 wells)
  • High sensitivity, measuring as little as 8.57 pg/ml of progesterone
  • Highly specific with negligible cross-reactivity to other steroids
  • High throughput format with results in < 3 hours for up to 38 samples in duplicate
  • Easy-to-use liquid color-coded reagents reduce errors
The Progesterone EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. This non-radioactive enzyme immunoassay is safe and reliable with ready-to-use liquid color-coded reagents to reduce errors.
Progesterone ELISA kit Kit graph
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Progesterone ELISA kit Kit graph

Product Specification

Sensitivity:8.57 pg/ml (range 15.62 - 500 pg/ml)
Assay Time:<3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of progesterone in culture supernatants, serum, and saliva from any species. Cited sample type includes plasma.
Species reactivity:Species independent
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C
Contents:GxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Steroid displacement reagent
Scientific Background:Progesterone is the major female sex hormone, and is responsible for reproductive-related activities such as breast glandular development, the endometrial aspects of the menstrual cycle, and the establishment and maintenance of pregnancy. In addition, progesterone also directs pregnancy support physiology including changes in carbohydrate, protein and lipid metabolism, thermoregulation, sodium reabsorption in renal tubules and the reduction of alveolar and atrial carbon dioxide partial pressures (pCO2). Progesterone is secreted in large amounts by the corpus luteum and by the extracellular conversion of cholesterol, cholestryl esters, adrenal steroids, prenenolone and pregnenolone sulfate. Small quantities are also secreted directly from the adrenal glands.
Technical Info/Product Notes:For an overview of cited samples, please click here

Application Note:
Validation of Two Steroid Hormone ELISA Kits with Ovine Blood Samples.

Product Literature References

Anxiolytic- and anxiogenic-like effects of Montanoa tomentosa (Asteraceae): Dependence on the endocrine condition: E. Estrada-Camarena, et al.; J. Ethnopharmacol. 241, 112006 (2019), Abstract;
Early luteal phase progestin concentration influences endometrial function in pregnant mares: T. Beyer, et al.; Theriogenology 125, 236 (2018), Abstract;
Environmental enrichment reverses Aβ pathology during pregnancy in a mouse model of Alzheimer's disease: S. Ziegler-Waldkirch, et al.; Acta Neuropathol. Commun. 6, 44 (2018), Abstract; Full Text
Oxidative and endoplasmic reticulum stress defense mechanisms of bovine granulosa cells exposed to heat stress: T.W. Alemu, et al.; Theriogenology 110, 130 (2018), Abstract; Full Text
The consequences of porcine IVM medium supplementation with follicular fluid become reflected in embryo quality, yield and gene expression patterns: P. Pawlak, et al.; Sci. Rep. 8, 15306 (2018), Abstract; Full Text
Validation of a blubber-based endocrine pregnancy test for humpback whales: L. Pallin, et al.; Conserv. Physiol. 6, coy031 (2018), Abstract; Full Text
Advanced age in mares affects endometrial secretion of arachidonic acid metabolites during equine subclinical endometritis: M.J. Siemieniuch, et al.; Theriogenology 103, 191 (2017), Application(s): ELISA using horse serum, Abstract;
Effect of antipsychotics on mitochondrial bioenergetics of rat ovarian theca cells: E. Elmorsy, et al.; Toxicol. Lett. 272, 92 (2017), Application(s): ELISA using culture supernatant, Abstract;
Endometrial mRNA expression of selected pro-inflammatory factors and mucins in repeat breeder cows with and without subclinical endometritis: K. Wagener, et al.; Theriogenology 90, 237 (2017), Application(s): ELISA using bovine plasma, Abstract;
In vivo transplantation of 3D encapsulated ovarian constructs in rats corrects abnormalities of ovarian failure: S. Sittajody, et al.; Nat. Commun. 8, 1858 (2017), Abstract; Full Text
Loss of predator aversion in female rats after Toxoplasma gondii infection is not dependent on ovarian steroids: S. Abdulai-Saiku & A. Vyas; Brain Behav. Immun. 65, 95 (2017), Application(s): ELISA using male rat samples with T. Gondi infection , Abstract;
Progesterone Modifies the Responsivity of the Amygdala-mPFC Connection in Male but not Female Wistar Rats: C.M. Contreras, et al.; Neurosci. Lett. 649, 1 (2017), Application(s): ELISA using rat plasma samples, Abstract;
Puerarin ameliorated the behavioral deficits induced by chronic stress in rats: Z.K. Qiu, et al.; Sci. Rep. 7, 6266 (2017), Application(s): EIA on rat tissue homogenate, Abstract; Full Text
Application of an innovative technique for unilateral ovariectomy in dairy cows: A. Rizzo, et al.; Vet. Rec. 179, 463 (2016), Abstract;
Effects of periovulatory gonadotrophin treatment on luteal function and endometrial expression of selected genes in cyclic pony mares: M. Köhne, et al.; Theriogenology 86, 2147 (2016), Application(s): ELISA using horse serum, Abstract;
Implantation and pregnancy outcome of Sprague-Dawley rats fed with low and high salt diet: G.O. Oludare, et al.; Middle East Fertil. Soc. J. 21, 228 (2016), Application(s): Measured rat serum progesterone levels,
Initiating Ovsynch on day 6 post estrus onset ± post AI early luteal phase GnRH treatment to improve ovarian and fertility response in repeat breeding crossbred cattle: N. Ahmed, et al.; Indian J. Anim. Reprod. 37, 5 (2016), Application(s): Serum progesterone ELISA, Full Text
Intravenous Immunoglobulin Suppresses Abortion Relates To an Increase in the CD44bright NK Subset in Recurrent Pregnancy Loss Model Mice: J. Tanaka, et al.; Biol. Reprod. 116, 138438 (2016), Application(s): Measurement of plasma P4 concentration and effect on peripheral immune cells, Abstract; Full Text
Maternal and fetal roles in bacterially induced preterm labor in the mouse: Y. Filipovich, et al.; Am. J. Obstet. Gynecol. 214, 386 (2016), Application(s): ELISA using mouse serum, Abstract; Full Text
Prostaglandin release by cultured endometrial tissues after challenge with lipopolysaccharide and tumor necrosis factor α, in relation to the estrous cycle, treatment with medroxyprogesterone acetate, and pyometra: E. Jursza-Piotrowska, et al.; Theriogenology 85, 1177 (2016), Abstract;
REGULATORY ROLE OF miR-20a DURING BOVINE OOCYTE MATURATION: E. Andreas, et al.; Reprod. Fertil. Dev. 29, 196 (2016), (Meeting abstract); Application(s): Bovine oocyte media, Full Text
Testosterone production by a leydig tumor cell line is suppressed by hyperthermia-induced endoplasmic reticulum stress in mice: J.H. Kim, et al.; Life Sci. 146, 184 (2016), Application(s): Measurement of progesterone in mouse mLTC-1 cell culture media, Abstract;
The PGF2α agonists luprostiol and d-cloprostenol reliably induce luteolysis in luteal phase mares without evoking clinical side effects or a stress response: J. Kuhl, et al.; Anim. Reprod. Sci. 168, 92 (2016), Application(s): Progesterone levels were measured in blood samples, Abstract;
The physiological expression of scavenger receptor SR-B1 in canine endometrial and placental epithelial cells and its potential involvement in pathogenesis of pyometra: C. Gabriel, et al.; Theriogenology 85, 1599 (2016), Application(s): Measured canine serum progesterone levels, Abstract;
Type of Inflammation Differentially Affects Expression of Interleukin 1β and 6, Tumor Necrosis Factor-α and Toll-Like Receptors in Subclinical Endometritis in Mares: M.J. Siemieniuch, et al.; PLoS One 11, e0154934 (2016), Application(s): Horse serum progesterone levels were measured, Abstract; Full Text
Altered secretion of selected arachidonic acid metabolites during subclinical endometritis relative to estrous cycle stage and grade of fibrosis in mares: K. Gajos, et al.; Theriogenology 84, 457 (2015), Application(s): ELISA using mare plasma, Abstract;
Determining intrafollicular concentrations of cortisol and progesterone in horses and the effects of cortisol on in vitro maturation of equine oocytes: D. Scarlet, et al. ; SBTE & AETE Meeting Abstracts (2015), Application(s): ELISA using horse samples, Full Text
Effects of season, age, sex and housing on salivary cortisol concentrations in horses: J. Aurich, et al.; Domest. Anim. Endocrinol. 52, 11 (2015), Application(s): ELISA, Abstract;
The prevalence of subclinical endometritis and intrauterine infections in repeat breeder cows: H. Pothmann, et al.; Theriogenology 83, 1249 (2015), Application(s): ELISA using cow plasma, Abstract;
Use of an anti-GnRF vaccine to suppress oestrus in crossbred Iberian female pigs: A. Dalmau, et al.; Theriogenology 84, 342 (2015), Application(s): ELISA using porcine serum , Abstract;
Uterine blood flow indices, antinuclear autoantibodies and unexplained recurrent miscarriage: A. Pietropolli, et al.; Obstet. Gynecol. Sci. 58, 453 (2015), Application(s): Measurement of human serum progesterone levels, Abstract;
Establishment and validation of a model for non-luteinized human mural granulosa cell culture: L. Ophir, et al.; Mol. Cell. Endocrinol. 384, 165 (2014), Application(s): EIA using culture supernatants from human cells, Abstract;
Placental origin of prostaglandin F2α in the domestic cat: M.J. Siemieniuch, et al.; Mediators Inflamm. 2014, 364787 (2014), Application(s): ELISA using feline placenta, Abstract; Full Text
Progesterone antagonist, RU486, represses LHCGR expression and LH/hCG signaling in cultured luteinized human mural granulosa cells: Y. Yung, et al.; Gynecol. Endocrinol. 30, 42 (2014), Application(s): EIA using culture supernatants from human cells, Abstract;
Treatment with human chorionic gonadotrophin before ovulation increases progestin concentration in early equine pregnancies: M. Köhne, et al.; Anim. Reprod. Sci. 149, 187 (2014), Application(s): ELISA using horse plasma, Abstract;
Tumor endothelial marker 1–specific DNA vaccination targets tumor vasculature: J. Facciponte, et al.; J. Clin. Invest. 124, 1497 (2014), Application(s): EIA using mouse serum, Abstract; Full Text
Cystic ovarian follicles and thyroid activity in the dairy cow: M. Mutinati, et al.; Anim. Reprod. Sci. 138, 150 (2013), Application(s): EIA using bovine serum, Abstract;
Engineered multilayer ovarian tissue that secretes sex steroids and peptide hormones in response to gonadotropins: S. Sittadjody, et al.; Biomaterials 34, 2412 (2013), Application(s): ELISA using supernatant of cultured primary rat ovaries, Abstract; Full Text
Female preference for males depends on reproductive physiology in the African cichlid fish Astatotilapia burtoni: M.R. Kidd, et al.; Gen. Comp. Endocrinol. 180, 56 (2013), Application(s): ELISA using aquarium water, Abstract;
Follicular stage-dependent regulation of apoptosis and steroidogenesis by prohibitin in rat granulosa cells: Q. Wang, et al.; J. Ovarian Res. 6, 23 (2013), Application(s): ELISA using supernatant of rat granulosa cells , Abstract; Full Text
Sex differences and similarities in the neuroendocrine regulation of social behavior in an African cichlid fish: L.A. O'Connell, et al.; Horm. Behav. 64, 468 (2013), Application(s): EIA using fish plasma, Abstract;
Sex differences in the effects of adolescent stress on adult brain inflammatory markers in rats: L.M. Pyter, et al.; Brain Behav. Immun. 30, 88 (2013), Application(s): EIA using rat plasma, Abstract; Full Text
The screening of everyday life chemicals in validated assays targeting the pituitary-gonadal axis: H. Tinwell, et al.; Regul. Toxicol. Pharmacol. 66, 184 (2013), Application(s): EIA using rat plasma, Abstract;
Social status predicts how sex steroid receptors regulate complex behavior across levels of biological organization: L.A. O'Connell, et al.; Endocrinology 153, 1341 (2012), Application(s): EIA using fish plasma, Abstract;
Fecal Hormones Measured within Giant Pacific Octopuses Enteroctopus dofleini: S. Larson & R. Anderson; J. Aquat. Anim. Health 22, 152 (2010), Application(s): EIA using octopus feces, Abstract;
Measuring multiple hormones from a single water sample using enzyme immunoassays: C. Kidd, et al.; Gen. Comp. Endocrinol. 165, 277 (2010), Application(s): EIA using fish plasma and water samples, Abstract;

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