Alternative size available: ADI-901-008 (5x96 wells)
Specific with low cross-reactivity to related steroids
Safe and non-radioactive enzymatic assay
Simple and color-coded reagents reduces error
Reliable and reproducible results day-after-day and lot-after-lot
Fully quantitative results that surpass semi-quantitative Western blot analysis
The 17β-Estradiol EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. This kit offers specific and save non-radioactive quantification of estradiol with ready-to-use liquid color-coded reagents to reduce error.
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Product Details
Alternative Name:
Estrogen
Sensitivity:
28.5 pg/ml (range 29.3 - 30,000 pg/ml)
Assay Time:
<3 hours
Applications:
ELISA, Colorimetric detection
Application Notes:
For the quantitative determination of 17β-Estradiol in culture supernatants and saliva from any species. For serum and plasma use ADI-900-174.
Biologically active estrogen, 17β-estradiol, is an important sex hormone, but also makes an important contribution to the high concentrations of estrogens, which are present in malignant breast tissues.
Improvement of Estrogen Deficiency Symptoms by the Intake of Long-Term Fermented Soybeans (Doenjang) Rich in Bacillus Species through Modulating Gut Microbiota in Estrogen-Deficient Rats: T. Zhang, et al.; Foods 12, 1143 (2023), Abstract;
Deficiency of Wnt10a causes female infertility via the β-catenin/Cyp19a1 pathway in mice: J.H. Zhang, et al.; Int. J. Med. Sci. 19, 701 (2022), Abstract;
A novel method to differentiate tonsil-derived mesenchymal stem cells in vitro into estrogen-secreting cells: H. Kim, et al.; Tissue Eng. Regen. Med. 18, 253 (2021), Application(s): ELISA using culture supernatants, Abstract; Full Text
Chronic irisin exposure decreases sexual incentive motivation in female rats: N. Ulker, et al.; Physiol. Behav. 232, 113341 (2021), Application(s): Rat serum, Abstract;
Hormone autocrination by vascularized hydrogel delivery of ovary spheroids to rescue ovarian dysfunctions: H.J. Yoon, et al.; Sci. Adv. 7, eabe8873 (2021), Abstract;
Kisspeptin/Kiss1r system and angiogenic and immunological mediators at the maternal-fetal interface of domestic cats: L.C. Santos, et al.; Biol. Reprod. 61, 1093 (2021), Abstract;
Neonatal androgenization in rats affects oocyte maturation: G. Anesetti & R. Chávez-Genaro; Reprod Sci. 28, 2799 (2021), Abstract;
Organochlorine pesticide dieldrin upregulate proximal promoter (PII) driven CYP19A1 gene expression and increases estrogen production in granulosa cells: D. Sharma, et al.; Reprod. Toxicol. 106, 103 (2021), Abstract;
Encapsulation of mesenchymal stem cells in 3D ovarian cell constructs promotes stable and long-term hormone secretion with improved physiological outcomes in a syngeneic rat model: S. Sittadjody, et al.; Ann. Biomed. Eng. 48, 1058 (2020), Application(s): ELISA using culture supernatants, Abstract; Full Text
Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors: W. Khaliq, et al.; Comput. Struct. Biotechnol. J. 18, 3678 (2020), Application(s): Human and rat plasma, Abstract; Full Text
A high level of TGF-B1 promotes endometriosis development via cell migration, adhesiveness, colonization, and invasiveness: U.K. Soni, et al.; Biol. Reprod. 100, 917 (2019), Abstract;
CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice: K. Zhang, et al.; J. Clin. Invest. 130, 2333 (2019), Abstract; Full Text
Hormonal balance and nutritional intake in elite tactical athletes: A.E. Jensen, et al.; Steroids 15, 108504 (2019), Application(s): ELISA using human plasma, Abstract;
Maternal nicotinamide riboside enhances postpartum weight loss, juvenile offspring development, and neurogenesis of adult offspring: P.H. Ear, et al.; Cell Rep. 26, 969 (2019), Application(s): ELISA using mouse and rat plasma samples, Abstract;
Effects of Supplementation of Human Endometriotic Fluids on In Vitro Mouse Preantral Follicle Culture: S.K. Kim, et al.; Reprod Sci. 25, 5 (2018), Abstract;
Inflammatory Responses are Sex Specific in Chronic Hypoxic-Ischemic Encephalopathy: A. Al Mamun, et al.; Cell Transplant. 27, 1328 (2018), Application(s): EIA using human serum, Abstract; Full Text
Welsh Onion Root (Allium fistulosum) Restores Ovarian Functions from Letrozole Induced-Polycystic Ovary Syndrome: Y.H. Lee, et al.; Nutrients 10, 1430 (2018), Application(s): Rat serum analysis, Abstract; Full Text
Dibutyl phthalate impairs steroidogenesis and a subset of LH-dependent genes in cultured human mural granulosa cell in vitro: M. Adir, et al.; Reprod. Toxicol. 69, 13 (2017), Application(s): Medium supernatant from human mural granulosa cells, Abstract;
Estrogen activation of microglia underlies the sexually dimorphic differences in Nf1 optic glioma-induced retinal pathology: J.A. Toonen, et al.; J. Exp. Med. 214, 17 (2017), Application(s): ELISA for human samples, Abstract; Full Text
Implantation and pregnancy outcome of Sprague-Dawley rats fed with low and high salt diet: G.O. Oludare, et al.; Middle East Fertil. Soc. J. 21, 228 (2016), Application(s): Measured rat serum estradiol levels,
Long-Term High Fat Diet Has a Profound Effect on Body Weight, Hormone Levels, and Estrous Cycle in Mice: T.R. Chacraborty, et al.; Med. Sci. Monit. 22, 1601 (2016), Application(s): ELISA using mouse serum, Abstract; Full Text
Optimizing multi-dimensional high throughput screening using zebrafish: L. Truong, et al.; Reprod. Toxicol. 65, 139 (2016), Application(s): Nominal exposure concentration measurement of 17β-estradiol and 17α-ethinylestradiol, Abstract;
Standard human chorionic gonadotropin versus double trigger for final oocyte maturation results in different granulosa cells gene expressions: a pilot study: J. Haas, et al.; Fertil. Steril. 106, 653 (2016), Application(s): Human Follicular Fluid, Abstract;
Automated and portable solid phase extraction platform for immuno-detection of 17β-estradiol in water: S. Heub, et al.; J. Chromatogr. A. 1381, 22 (2015), Application(s): ELISA using water samples, Abstract;
Arsenic-induced cancer cell phenotype in human breast epithelia is estrogen receptor-independent but involves aromatase activation: Y. Xu, et al.; Arch. Toxicol. 88, 263 (2014), Application(s): ELISA using culture supernatant, Abstract; Full Text
Estradiol treatment promotes cardiac stem cell (CSC)-derived growth factors, thus improving CSC-mediated cardioprotection after acute ischemia/reperfusion: L. Wang, et al.; Surgery 156, 243 (2014), Application(s): ELISA using culture supernatant and cell perfusate solution, Abstract;
Expression and activity of Rac1 is negatively affected in the dehydroepiandrosterone induced polycystic ovary of mouse: V. Maurya, et al.; J. Ovarian Res. 7, 32 (2014), Application(s): EIA using mouse ovarian tissue, Abstract; Full Text
GnRH Agonist vs. hCG for Triggering of Ovulation – Differential Effects on Gene Expression in Human Granulosa Cells: J. Haas, et al.; PLoS One 9, e90359 (2014), Application(s): Human Serum, Abstract; Full Text
Tumor endothelial marker 1–specific DNA vaccination targets tumor vasculature: J. Facciponte, et al.; J. Clin. Invest. 124, 1497 (2014), Application(s): EIA using mouse serum, Abstract; Full Text
Engineered multilayer ovarian tissue that secretes sex steroids and peptide hormones in response to gonadotropins: S. Sittadjody, et al.; Biomaterials 34, 2412 (2013), Application(s): ELISA using supernatant of cultured primary rat ovaries, Abstract; Full Text
Female preference for males depends on reproductive physiology in the African cichlid fish Astatotilapia burtoni: M.R. Kidd, et al.; Gen. Comp. Endocrinol. 180, 56 (2013), Application(s): ELISA using aquarium water, Abstract;
Follicular stage-dependent regulation of apoptosis and steroidogenesis by prohibitin in rat granulosa cells: Q. Wang, et al.; J. Ovarian Res. 6, 23 (2013), Application(s): ELISA using supernatant of rat granulosa cells , Abstract; Full Text
Gender dimorphism in regulation of plasma proteins in streptozotocin-induced diabetic rats: J.W. Choi, et al.; Proteomics 13, 2482 (2013), Application(s): Rat plasma, Abstract;
MEN1 is a melanoma tumor suppressor that preserves genomic integrity by stimulating transcription of genes that promote homologous recombination-directed DNA repair: M. Fang, et al.; Mol. Cell. Biol. 33, 2635 (2013), Application(s): ELISA using cell lysates, Abstract; Full Text
Preliminary findings of fecal gonadal hormone concentrations in six captive sea otters (Enhydra lutris) after deslorelin implantation: S. Larson, et al.; Zoo Biol. 32, 307 (2013), Application(s): EIA using sea otter feces, Abstract;
Sex steroid hormones modulate responses to social challenge and opportunity in males of the monogamous convict cichlid, Amatitliana nigrofasciata: A. Sessa, et al.; Gen. Comp. Endocrinol. 189, 59 (2013), Application(s): EIA using fish plasma, Abstract;
Fecal Hormones Measured within Giant Pacific Octopuses Enteroctopus dofleini: S. Larson & R. Anderson; J. Aquat. Anim. Health 22, 152 (2010), Application(s): EIA using octopus feces, Abstract;
Measuring multiple hormones from a single water sample using enzyme immunoassays: C. Kidd, et al.; Gen. Comp. Endocrinol. 165, 277 (2010), Application(s): EIA using fish plasma and water samples, Abstract;
Sex steroid-mediated reprogramming of vascular smooth muscle cells to stem cells and neurons: possible utilization of sex steroid combinations for regenerative treatment without utilization of in vitro developed stem cells: A. Bukovsky; Cell Cycle 8, 4079 (2009), Application(s): EIA using ovarian epithelial cells, Abstract;
Comparison of three enzyme immunoassays for measuring 17beta-estradiol in flushed dairy manure wastewater: T. Hanselman, et al.; J. Environ. Qual. 33, 1919 (2004), Application(s): EIA using livestock feces, water samples, Abstract;
