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PGE2 ELISA kit

Highly sensitive PGE2 ELISA kit for inflammation and eicosanoid research.
 
ADI-900-001 96 wells 362.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-901-001 (5x96 wells)
  • Highly sensitive measurement, detecting as little as 13.4 pg/ml PGE2
  • Higher throughput format with results in <3 hrs for up to 37 samples in duplicate
  • Widely cited in peer reviewed literature
  • Ready-to-use liquid color-coded reagents reduce errors
  • Reproducible results day-after-day and lot-after-lot
The PGE2 EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Screen inhibitors of COX II activity by measuring the levels of downstream PGE2. Commercially available since 1992, this kit is widely cited in peer-reviewed publications. The non-radioactive ready-to-use liquid color-coded reagents reduce errors.
900-001 lot-to-lot
ADI-900-001 std curve
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900-001 lot-to-lot ADI-900-001 std curve

Product Specification

Alternative Name:Prostaglandin E2
 
Sensitivity:13.4 pg/ml (range 39.1 - 2,500 pg/ml)
 
Assay Time:<3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of PGE2 in culture supernatants, serum, saliva, urine, and whole blood from any species. Cited sample types include cerebral spinal fluid, dialysate, gingival crevicular fluid, peritoneal exudate fluid and peritoneal exudate cell supernatant, plasma, and tissue.
 
Species reactivity:Species independent
 
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:GxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.
 

Product Literature References

Colon cancer-derived myofibroblasts increase endothelial cell migration by glucocorticoid-sensitive secretion of a pro-migratory factor: Z. Drebert, et al.; Vascul. Pharmacol. 89, 19 (2017), Application(s): Determine the concentrations of prostanoids in conditioned medium from myofibroblasts and HUVECs, and in HUVEC lysates, Abstract;
Expression of toll-like receptor-1 by bradykinin in human gingival fibroblasts: J.A. Arreguin-Cano T; Int. J. Clin. Exp. Pathol. 10, 1283 (2017), Application(s): Measurement of PGE2 in supernatant., Full Text
Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome: H.C. Clark, et al.; Stem Cell Res. Ther. 8, 69 (2017), Application(s): Measurement of PGE2 in supernatant of human and feline cells., Abstract; Full Text
Novel augmentation by bufalin of protein kinase C-induced cyclooxygenase-2 and IL-8 production in human breast cancer cells: H.T. Chen, et al.; Innate Immun. 23, 54 (2017), Abstract; Full Text
Prostaglandin E2 stimulates the expression of cumulus expansion-related genes in pigs: the role of protein kinase B: M. Blaha, et al.; Prostaglandins Other Lipid Mediat. 130, 38 (2017), Application(s): ELISA using pig samples, Abstract;
A positive feedback loop between progesterone and microsomal prostaglandin E synthase-1-mediated PGE2 promotes production of both in mouse granulosa cells: K. Tamura, et al.; Prostaglandins Other Lipid Mediat. 123, 56 (2016), Application(s): Measurement of PGE2 in the culture media, Abstract;
Anti-inflammatory effects of 6′-O-acetyl mangiferin from Iris rossii Baker via NF-κb signal blocking in lipopolysaccharide-stimulated RAW 264.7 cells: J.H. Jang, et al.; Chem. Biol. Interact. 2797, 30303 (2016), Application(s): Determination of PGE2 production, Abstract;
Dimethyl fumarate activates the prostaglandin EP2 receptor and stimulates cAMP signaling in human peripheral blood mononuclear cells: S.E. Fiedler, et al.; Biochem. Biophys. Res. Commun. 475, 19 (2016), Application(s): Supernatant PGE2 was measured, Abstract;
Directly interact with Keap1 and LPS is involved in the anti-inflammatory mechanisms of (-)-epicatechin-3-gallate in LPS-induced macrophages and endotoxemia: Y.S. Chiou, et al.; Free. Radic. Biol. Med. 94, 1 (2016), Application(s): Measurement of prostaglandin E2 (PGE2) level in culture medium, Abstract;
Effects of Almond- and Olive Oil-Based Docosahexaenoic- and Vitamin E-Enriched Beverage Dietary Supplementation on Inflammation Associated to Exercise and Age: X. Capo, et al.; Nutrients 8, 619 (2016), Application(s): Cytokine, Eicosanoids and Adhesion Molecules Determination, plasma, Abstract; Full Text
Enhanced anti-inflammatory activity of brown seaweed Laminaria japonica by fermentation using Bacillus subtilis: H.T.V. Lin, et al.; Process Biochem. (2016), Application(s): Measurement of prostaglandin E2 (PAGE2), cell supernatant,
Grid-like surface structures in thermoplastic polyurethane induce anti-inflammatory and anti-fibrotic processes in bone marrow-derived mesenchymal stem cells: Y. Roger, et al.; Colloids Surf. B Biointerfaces 148, 104 (2016), Application(s): ELISA using human bone marrow-derived mesenchymal stem cell supernatant, Abstract;
Implantation and pregnancy outcome of Sprague-Dawley rats fed with low and high salt diet: G.O. Oludare, et al.; Middle East Fertil. Soc. J. (2016), Application(s): Measured rat serum PGE2 levels,
In vitro bioactive properties of phlorotannins recovered from hydrothermal treatment of Sargassum muticum: M.P. Casas, et al.; Sep. Purif. Technol. 167, 117 (2016), Application(s): Determination of the amount of PGE2 in thawed plasma supernatants,
Isolation and Identification of a Flavone Apigenin from Marine Red Alga Acanthophora spicifera with Antinociceptive and Anti-Inflammatory Activities: G.A. El Shoubaky, et al.; J. Exp. Neurosci. 10, 21 (2016), Application(s): TNF-α measured in mouse blood samples, Abstract; Full Text
Local intra-uterine Ang-(1-7) infusion attenuates PGE2 and 6-keto PGF1α in decidualized uterus of pseudopregnant rats: K.B. Brosnihan, et al.; Reprod. Biol. Endocrinol. 14, 68 (2016), Application(s): Assaying tissue extracts, Abstract; Full Text
MRI-Based Assessment of Intralesional Delivery of Bone Marrow-Derived Mesenchymal Stem Cells in a Model of Equine Tendonitis: A. Scharf, et al.; Stem Cells Int. 2016, 1 (2016), Application(s): Assessing the ability of MSCs to modulate the inflammatory response, Abstract; Full Text
Resokaempferol-mediated anti-inflammatory effects on activated macrophages via the inhibition of JAK2/STAT3, NF-κB and JNK/p38 MAPK signaling pathways: Q. Yu, et al.; Int. Immunopharmacol. 38, 104 (2016), Application(s): PGE2 was measured in mouse supernatant, Abstract;
The Prostaglandin E2-EP3 Receptor Axis Regulates Anaplasma phagocytophilum-Mediated NLRC4 Inflammasome Activation: X. Wang, et al.; PLoS One 12, e1005803 (2016), Application(s): PGE2 measurement in cell lysates, Abstract; Full Text
Antagonizing Effects of Aspartic Acid against Ultraviolet A-Induced Downregulation of the Stemness of Human Adipose Tissue-Derived Mesenchymal Stem Cells: K. Jung, et al.; PLoS One 10, e0124417 (2015), Application(s): ELISA using human culture supernatant , Abstract; Full Text
Anti-inflammatory effect of desoxo-narchinol-A isolated from Nardostachys jatamansi against lipopolysaccharide: J.Y. Shin, et al.; Int. Immunopharmacol. 29, 730 (2015), Application(s): PGE2 level was measured by ELISA in mice, Abstract;
Effectiveness of the combinational treatment of Laminaria japonica and Cistanche tubulosa extracts in hair growth: K. Shin, et al.; Lab. Anim. Res. 31, 24 (2015), Application(s): ELISA using mouse exudate, Abstract; Full Text
Insight into the molecular mechanism of an herbal injection by integrating network pharmacology and in vitro: Y.M. Ma, et al.; J. Ethnopharmacol. 173, 91 (2015), Application(s): ELISA using mouse RAW264.7 cells , Abstract;
Lipin-1 contributes to modified low-density lipoprotein-elicited macrophage pro-inflammatory responses: A.R. Navratil, et al. ; Atherosclerosis 242, 424 (2015), Application(s): ELISA using mouse cell culture supernatant, Abstract;
Molecular characterization and knock-down of salmon louse (Lepeophtheirus salmonis) prostaglandin E synthase: C. Eichner, et al.; Exp. Parasitol. 159, 79 (2015), Application(s): ELISA Kit measuring PGE2 levels in salmon lice, Abstract;
Prostaglandin E2 Production and T Cell Function in Mouse Adenovirus Type 1 Infection following Allogeneic Bone Marrow Transplantation: M.K. McCarthy, et al.; PLoS One 10, e0139235 (2015), Application(s): PGE2 concentration in bronchoalveolar lavage fluid , Abstract; Full Text
Role of gastric acid inhibition, prostaglandins and endogenous-free thiol groups on the gastroprotective effect of a proteolytic fraction from Vasconcellea cundinamarcensis latex: A. Silva, et al.; J. Pharm. Pharmacol. 67, 133 (2015), Application(s): EIA using rat tissue samples, Abstract; Full Text
Supplement of 5-hydroxytryptophan before induction suppresses inflammation and collagen-induced arthritis: T.H. Yang, et al. ; Arthritis. Res. Ther. 17, 364 (2015), Application(s): Measurement of PGE2 in mice serum samples, Abstract; Full Text
The cancer pain related factors affected by celecoxib together with cetuximab in head and neck squamous cell carcinoma: Y. Yang, et al.; Biomed. Pharmacother. 70, 181 (2015), Application(s): ELISA using human culture medium, Abstract;
The Receptor CMRF35-Like Molecule-1 (CLM-1) Enhances the Production of LPS-Induced Pro-Inflammatory Mediators during Microglial Activation: A. Ejarque-Ortiz, et al.; PLoS One 10, e0123928 (2015), Application(s): ELISA using mouse microglial supernatant, Abstract; Full Text
Inhibition of COX-2 and PGE2 in LPS-stimulated RAW264.7 cells by lonimacranthoide VI, a chlorogenic acid ester saponin: F. Guan, et al.; Biomed. Rep. 2, 760 (2014), Application(s): Assay of LPS-stimulated RAW264.7 macrophages, Abstract; Full Text
Possible roles of COX-1 in learning and memory impairment induced by traumatic brain injury in mice: J. Shang, et al.; Braz. J. Med. Biol. Res. 47, 1050 (2014), Application(s): Analysis of PGE2 levels in mouse hippocampus tissue, Abstract; Full Text
Prostaglandin E2 promotes features of replicative senescence in chronically activated human CD8+ T cells: J.P. Chou, et al.; PLoS One 9, e99432 (2014), Application(s): EIA using cell culture supernatants, Abstract; Full Text
Anti-inflammatory, antioxidative and matrix metalloproteinase inhibitory properties of 20(R)-ginsenoside Rh2 in cultured macrophages and keratinocytes: W.Y. Choi, et al.; J. Pharm. Pharmacol. 65, 310 (2013), Application(s): EIA using culture supernatants, Abstract;
Colorectal carcinoma-derived fibroblasts modulate natural killer cell phenotype and antitumor cytotoxicity: T. Li, et al.; Med. Oncol. 30, 663 (2013), Application(s): EIA using culture supernatants, Abstract;
Melanoma-educated CD14+ cells acquire a myeloid-derived suppressor cell phenotype through COX-2-dependent mechanisms: Y. Mao, et al.; Cancer Res. 73, 3877 (2013), Application(s): EIA using culture supernatants, Abstract;
Microenvironment generated during EGFR targeted killing of pancreatic tumor cells by ATC inhibits myeloid-derived suppressor cells through COX2 and PGE2 dependent pathway: A. Thakur, et al.; J. Transl. Med. 11, 35 (2013), Application(s): EIA using culture supernatants from 3D co-cultures, Abstract; Full Text
Prostaglandin E2 alteration in contraceptive consumers: as a risk factor for inflammatory diseases: M.S. Pour, et al.; Arch. Gynecol. Obstet. 287, 1031 (2013), Application(s): EIA using human serum, Abstract;
Transmigration of Neural Stem Cells across the Blood Brain Barrier Induced by Glioma Cells: M. Díaz-Coránguez, et al.; PLoS One 8, e60655 (2013), Application(s): Quantification was done in 4 day CM derived from rat astrocytes and glioma C6 cells using ELISA, Abstract; Full Text
XH-14, a novel danshen methoxybenzo[b]furan derivative, exhibits anti-inflammatory properties in lipopolysaccharide-treated RAW 264.7 cells: G.M. Park, et al.; J. Inflamm. 10, 1 (2013), Application(s): EIA using culture supernatants, Abstract; Full Text
Acacetin protects dopaminergic cells against 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine-induced neuroinflammation in vitro and in vivo: H.G. Kim, et al.; Biol. Pharm. Bull. 35, 1287 (2012), Application(s): EIA using culture supernatants, Abstract; Full Text
Hepatocellular carcinoma-associated fibroblasts trigger NK cell dysfunction via PGE2 and IDO: T. Li, et al.; Cancer Lett. 318, 154 (2012), Application(s): EIA using culture supernatants, Abstract;
Lycorine inhibits lipopolysaccharide-induced iNOS and COX-2 up-regulation in RAW264.7 cells through suppressing P38 and STATs activation and increases the survival rate of mice after LPS challenge: J. Kang, et al.; Int. Immunopharmacol. 12, 249 (2012), Application(s): EIA using culture supernatants, Abstract;
Matrix metalloproteinase-19 is a key regulator of lung fibrosis in mice and humans: G. Yu, et al.; Am. J. Respir. Crit. Care Med. 186, 752 (2012), Application(s): PGE2 from mice BAL was measured, Abstract;
Delta6-desaturase (FADS2) deficiency unveils the role of omega3- and omega6-polyunsaturated fatty acids: W. Stoffel, et al.; EMBO J. 27, 2281 (2008), Application(s): EIA using tissue, Abstract;
Anti-inflammatory effects of isoflavone powder produced from soybean cake: T.H. Kao, et al.; J. Agric. Food Chem. 55, 11068 (2007), Application(s): EIA using peritoneal exudate fluid and peritoneal exudate cell supernatant, Abstract;
Celecoxib inhibits Cdx2 expression and prevents gastric cancer in Helicobacter pylori-infected Mongolian gerbils: S. Futagami, et al.; Digestion 74, 187 (2006), Abstract;
Spinal inflammatory hyperalgesia is mediated by prostaglandin E receptors of the EP2 subtype: H. Reinold, et al.; J. Clin. Invest. 115, 673 (2005), Application(s): PGE2 from the thoracolumbar segment of the spinal cord was measured, mice, Abstract; Full Text
Effect of a selective cyclooxygenase-2 inhibitor, nimesulide, on the growth of lung tumors and their expression of cyclooxygenase-2 and peroxisome proliferator- activated receptor-gamma: M.S. Shaik, et al.; Clin. Cancer Res. 10, 1521 (2004), Application(s): EIA using mouse tissue, Abstract;
Nonopioid actions of intrathecal dynorphin evoke spinal excitatory amino acid and prostaglandin E2 release mediated by cyclooxygenase-1 and -2: L. Koetzner, et al.; J. Neurosci. 24, 1451 (2004), Application(s): EIA using dialysate, Abstract;
Differential expression of platelet-activating factor acetylhydrolase in macrophages and monocyte-derived dendritic cells: S. Al-Darmaki, et al.; J. Immunol. 170, 167 (2003), Application(s): EIA using human culture supernatants, Abstract;
Inhibition of cyclooxygenase-2 by rofecoxib attenuates the growth and metastatic potential of colorectal carcinoma in mice: M. Yao, et al.; Cancer Res. 63, 586 (2003), Application(s): Concentration of PGE2 in splenic tumor cystosolic fractions were determined using mouse tissue, Abstract;
Microsomal prostaglandin E synthase-1 is a major terminal synthase that is selectively up-regulated during cyclooxygenase-2-dependent prostaglandin E2 production in the rat adjuvant-induced arthritis model: D. Claveau, et al.; J. Immunol. 170, 4738 (2003), Application(s): PGE2 production was determined by enzyme immunoassay analysis using rat tissue, Abstract; Full Text
Increased levels of the pro-inflammatory prostaglandin PGE2 in CSF from ALS patients: G. Almer, et al.; Neurology 58, 1277 (2002), Application(s): EIA using cerebral spinal fluid, Abstract;
The effects of periodontal therapy on intracrevicular prostaglandin E2 concentrations and clinical parameters in pregnancy: F. Yalcin, et al.; J. Periodontol. 73, 173 (2002), Application(s): EIA using gingival crevicular fluid, Abstract;
Promoter methylation regulates Helicobacter pylori-stimulated cyclooxygenase-2 expression in gastric epithelial cells: M. Akhtar, et al.; Cancer Res. 61, 2399 (2001), Application(s): EIA using cell culture supernatant, Abstract; Full Text
Inflammatory agonists induce cyclooxygenase type 2 expression by human neutrophils: C.G. Maloney, et al.; J. Immunol. 160, 1402 (1998), Application(s): EIA using human cell culture supernatant, Abstract;
Selective cyclooxygenase-2 inhibition by nimesulide in man: L. Cullen, et al.; J. Pharmacol. Exp. Ther. 287, 578 (1998), Application(s): EIA using plasma, Abstract;

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