Enzo Life Sciences continues to expand its unique offering of ProteoStat® protein refolding, stability and aggregation detection products for characterization, formulation and process development of protein therapeutics. ProteoStat® products are designed to facilitate cost efficient production of therapeutic antibodies and recombinant proteins, helping developers achieve maximum purity, activity, and yield.
ProteoStat® products rely on a proprietary dye technology that fluoresces when it binds to protein aggregates. In solution, when its rotation is not constrained, the excited molecular rotor dye is basically non-fluorescent. When the dye locks into the quaternary structure of protein aggregates, it becomes highly fluorescent, similar to ethidium bromide fluorescence upon intercalation into the three-dimensional structure of DNA.
New to the portfolio of ProteoStat® assays is the Protein Refolding and Aggregation Sensing kit. The assay uses a Design of Experiments matrix to identify optimal protein refolding conditions within common buffer formulations and additives. When paired with a screen for protein function, the fluorescent aggregation readout quickly identifies formulations that avoid aggregation, while maximizing recovery of functional protein.